Aedes aegypti abundance and insecticide resistance profiles in the Applying Wolbachia to Eliminate Dengue trial.

The Applying Wolbachia to Eliminate Dengue (AWED) trial was a parallel cluster randomised trial that demonstrated Wolbachia (wMel) introgression into Ae. aegypti populations reduced dengue incidence. In this predefined substudy, we compared between treatment arms, the relative abundance of Ae.

Efficacy of Wolbachia-Infected Mosquito Deployments for the Control of Dengue.

Background: mosquitoes infected with the Mel strain of are less susceptible than wild-type to dengue virus infection.

Methods: We conducted a cluster-randomized trial involving releases of Mel-infected mosquitoes for the control of dengue in Yogyakarta, Indonesia. We randomly assigned 12 geographic clusters to receive deployments of Mel-infected (intervention clusters) and 12 clusters to receive no deployments (control clusters).

Reduced dengue incidence following deployments of -infected in Yogyakarta, Indonesia: a quasi-experimental trial using controlled interrupted time series analysis.

mosquitoes stably transfected with the intracellular bacterium ( Mel strain) have been deployed for biocontrol of dengue and related arboviral diseases in multiple countries. Field releases in northern Australia have previously demonstrated near elimination of local dengue transmission from -treated communities, and pilot studies in Indonesia have demonstrated the feasibility and acceptability of the method. We conducted a quasi-experimental trial to evaluate the impact of scaled releases on dengue incidence in an endemic setting in Indonesia.

Stable establishment of wMel Wolbachia in Aedes aegypti populations in Yogyakarta, Indonesia.

The successful establishment of the wMel strain of Wolbachia for the control of arbovirus transmission by Aedes aegypti has been proposed and is being implemented in a number of countries. Here we describe the successful establishment of the wMel strain of Wolbachia in four sites in Yogyakarta, Indonesia. We demonstrate that Wolbachia can be successfully introgressed after transient releases of wMel-infected eggs or adult mosquitoes.