Publications by authors named "Ines Raineri"
The aims of this study were first, to systematically assess the inter-observer reproducibility of mean Epidermal Growth Factor Receptor (EGFR) gene copy number (MCN) in histological and cytological specimens from lung and non-lung cancers, second to compare the performance of this quantitative approach to the current Colorado criteria for the assessment of fluorescence in situ hybridization (FISH) positivity and third to develop a model to convert cytology into histology MCN. EGFR FISH analysis was performed on 170 histological and 153 cytological specimens. The MCN and Colorado criteria were assessed by two independent observers and agreement evaluated using Bland-Altman plots and kappa values.
View Article and Find Full Text PDFA 24-year-old man was admitted for a painful gingival ulcer. Histology and immunohistochemistry of a lesional biopsy revealed the diagnosis of Langerhans cell histiocytosis (LCH). To rule out multifocal disease, a complete staging was performed.
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- The study explores how post-transcriptional mechanisms impact early differentiation of embryonic stem (ES) cells, which is less understood compared to transcription factors.
- A new system using small hairpin (sh)RNAs allows targeted gene silencing in ES cells, enabling researchers to study specific gene functions related to differentiation.
- The research highlights Brf1 as a significant regulator in cardiomyocyte formation, suggesting that post-transcriptional regulation plays a crucial role in early development and has potential implications for regenerative medicine.
Article Synopsis
- HT1080 cells expressing GFP linked to AU-rich elements serve as a model to study mRNA stability by tracking fluorescence intensity changes.
- The study investigates how small interfering RNAs (siRNAs) targeting AU-binding proteins (AUBPs) impact mRNA stability, revealing that targeting HuR or BRF1 affects fluorescence levels, indicating their roles as stabilizers or destabilizers.
- Interestingly, selectively reducing certain AUF1 isoforms (p40AUF1/p45AUF1) significantly increases fluorescence and stabilizes mRNA, suggesting that the specific isoform levels, not just the total AUF1 amount, influence mRNA stability in these cells.
Article Synopsis
- The study focuses on identifying regulators of AU-rich element (ARE)-dependent mRNA turnover using a mutagenized cell line, slowC, that cannot degrade certain cytokine mRNA.
- Researchers utilized a GFP reporter construct and flow cytometry to rescue slowC with cDNA from a retroviral library, identifying the crucial gene BRF1, a zinc finger protein important for mRNA decay.
- The findings show that BRF1 enhances mRNA degradation and counteracts stabilization by PI3-kinase, providing evidence of its role through various methodologies, including siRNA and mutational analysis.