Publications by authors named "Ines Krohn"

Article Synopsis
  • * Researchers examined the microbiome of the macroalga Fucus vesiculosus over 25 days, finding that Pseudomonadota, particularly Marinomonas and Vibrio, dominated the microbial community, while Bacteroidota showed the highest potential for cell wall degradation despite being less abundant.
  • * Two α-L-fucosidase enzymes (FUJM18 and FUJM20) were cloned and characterized, showing high activity at elevated temperatures, suggesting they could be used to create a synthetic enzyme cocktail for large-scale degradation of algae.
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are ubiquitous Gram-negative bacteria found in both natural and clinical environments. It is a remarkably adaptable species capable of thriving in various environments, thanks to the plasticity of its genome and a diverse array of genes that encode a wide range of functions. Among these functions, one notable trait is its remarkable ability to resist various antimicrobial agents, primarily through mechanisms that regulate the diffusion across cell membranes.

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Article Synopsis
  • - Biofilms can resist traditional antibiotics, prompting the search for new antimicrobials from unique sources like aquatic microorganisms, leading to the analysis of microalgae-bacteria communities for potential antimicrobial enzymes.
  • - A promising enzyme candidate called Dlh3 was found to inhibit biofilm development of the fish pathogen Edwardsiella anguillarum by up to 54.5% and showed positive effects on self-defense genes in response to its application.
  • - Dlh3 demonstrates biotechnological potential for aquaculture because it effectively inhibits harmful biofilms without negatively affecting the health or growth of a relevant fish cell model (CHSE-214).
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  • * Focus is on Stenotrophomonas maltophilia, which forms harmful biofilms on medical devices, and how a supernatant from stony coral can reduce biofilm formation by 40%.
  • * Researchers identified metalloproteases in the supernatant that contribute to the anti-biofilm effects, highlighting their potential as new antimicrobial agents in health care.
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Based on previous research, related to detailed insight into mutualistic collaboration of microalga and its microbiome, we established an artificial plant-bacteria system of the microalga MZCH 672 and the bacterial isolate sp. HH091. The bacteria, affiliated with the phylum Bacteroidota, strongly stimulated growth of the microalga when it was added to axenic algal cultures.

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Sustainable use of natural products is one of the key challenges for the future. An increasing focus is on marine organic matter, mostly algae. New biotechnological tools for processing high amounts of micro- and macroalgae are necessary for efficient industrial degradation of marine matter.

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The mining of genomes from non-cultivated microorganisms using metagenomics is a powerful tool to discover novel proteins and other valuable biomolecules. However, function-based metagenome searches are often limited by the time-consuming expression of the active proteins in various heterologous host systems. We here report the initial characterization of novel single-subunit bacteriophage RNA polymerase, EM1 RNAP, identified from a metagenome data set obtained from an elephant dung microbiome.

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Article Synopsis
  • Microalgae, like Scenedesmus quadricauda, have a specialized microbiota consisting of less than 10 distinct bacterial species, primarily including Variovorax, Porphyrobacter, and Dyadobacter.
  • Dyadobacter plays a crucial role in promoting algal growth by producing polysaccharide-modifying enzymes and expressing important proteins, while also showing higher levels of quorum-quenching proteins and vitamin biosynthesis compared to the other bacteria.
  • This study enhances our understanding of the mutualistic relationship between microalgae and their associated bacteria, emphasizing their interactions and the sharing of vital resources like vitamins and carbon.
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Microalgae comprise a phylogenetically very diverse group of photosynthetic unicellular pro- and eukaryotic organisms growing in marine and other aquatic environments. While they are well explored for the generation of biofuels, their potential as a source of antimicrobial and prebiotic substances have recently received increasing interest. Within this framework, microalgae may offer solutions to the societal challenge we face, concerning the lack of antibiotics treating the growing level of antimicrobial resistant bacteria and fungi in clinical settings.

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Presently, two Chlorella sorokiniana strains sampled during summer (CS-S) and winter (CS-W) from a maturation pond and isolated by dominance were studied on their behavior on temperature and light extremes in batch experiments. Although both strains showed no differences in their tolerance of temperatures up to 45 °C, the growth rates, pigment contents and fatty acid compositions in response to PAR at 700 and 1,500 µmol msec differed. CS-W was less affected by photoinhibition and maintained constantly high growth rates.

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  • Microbial contamination of fuels, caused by various bacteria and fungi, negatively affects fuel quality and equipment performance due to biofouling and corrosion.
  • The study involved analyzing six metagenomes, one transcriptome, and over 1,200 samples from fuel sources to monitor and understand microbial contamination.
  • Findings revealed metabolic relationships between bacteria and fungi, with significant gene activity related to kerosene degradation and biofilm formation, highlighting a range of enzymes involved in both polymer degradation and bio-corrosion processes.
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, also known as , is a promising green microalga for sustainable production of microalga products, including valuable compounds such as astaxanthin, β-carotene, and lutein, polysaccharides such as β-glucan, and polyunsaturated fatty acids. Here, we report the draft whole-genome sequence of SAG 38.81.

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