Laboratory host systems for extraneous agent testing in avian live virus vaccines: problems encountered.

The regulation for batch testing of avian live viral vaccines for extraneous agents was modified recently in order to reduce animal use, and routine batch testing had to be adapted to this new regulation. As a result, however, some tests have become more complicated and time-consuming. Due to systematic technical problems, the new methods required could only be applied to a small proportion of the batches produced.