Publications by authors named "Ines Garteizgogeascoa Suner"
Fluorescent Tagging of Endogenous FOXO for Live Imaging and Pull-Down Assays.
Methods Mol Biol
November 2024
Proteins are the major and most diverse biomolecules, directing all activities of a cell. For this reason, visualizing protein expression, localization, and dynamics is fundamental in biology. In most cases, protein visualization relies on the overexpression of fluorescently tagged proteins which may not recapitulate endogenous expression pattern and dynamics.
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- Calcium plays a vital role in bodily functions and is essential for cell entry through various channels, but studying it in living tissues has been challenging due to limited methods.
- The study introduces GreenT-ECs, highly sensitive genetically encoded calcium biosensors with a wide range of calcium binding affinities (0.8 mM to 2.9 mM), perfect for tracking calcium in extracellular fluids.
- Validation in rodent neurons and transgenic zebrafish demonstrates that GreenT-ECs effectively monitor calcium levels, making them promising tools for observing calcium dynamics in live tissues and organisms.
Thyroid hormone (T3) and its nuclear receptors (TR) are important regulators of energy expenditure and adaptive thermogenesis, notably through their action in the brown adipose tissue (BAT). However, T3 acts in many other peripheral and central tissues which are also involved in energy expenditure. The general picture of how T3 regulates BAT thermogenesis is currently not fully established, notably due to the absence of extensive omics analyses and the lack of specific mice model.
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