Successful live birth from oocytes after more than 14 years of cryopreservation.

Objective: To report a birth of a healthy girl after long-term oocyte cryopreservation by slow cooling in sodium depleted medium.

Design: Clinical application.

Setting: University Affiliated, Private IVF center.

[Uterine subrogation: medical and legal aspects of the first legally supported case in Argentina].

A woman aged 38 was referred to this center for surrogacy treatment, after subtotal ablation of her uterus due to a severe postpartum hemorrhage. Her hormonal profiles and ovarian structure were normal. The husband proved fertile and semen analysis was normal.

Live birth of twins after IVF of oocytes that were cryopreserved almost 12 years before.

A 45-year-old woman received embryos from IVF by intracytoplasmic sperm injection (ICSI) with her own oocytes that were cryopreserved (slow freezing in a low-sodium medium) 11 years and 7 and a half months before, when she was 33 years old. From seven metaphase-II oocytes thawed, five survived, four were fertilized after ICSI and two cleaving embryos were transferred on day 3. A diamniotic dichorionic term pregnancy was achieved, ending with the delivery of two healthy girls.