[Malignant lymphomas in primates and the principles of their morphological diagnosis (on a baboon model of malignant lymphomas)].

114 baboons with non-Hodgkin lymphoma (NHL) were examined, by using the morphoimmunological phenotyping technique. According to the Kiel classification, 14 types of non-Hodgkin lymphomas of high and low malignancy are identified and described. These include 8 T-cell lymphomas and six B-cell NHL.

[Development of a method for creating a colony of Macaca mulatta, free of herpes B virus infection].

To form the colony of monkeys, free of herpes B virus, the serological study was made with the aim of finding out the carriers of this virus. 482 rhesus macaques (Macaca mulatta) from the Adler monkey house were examined for the presence of antibodies to herpes B virus by the method of point immunoblotting with the use of Herpes virus simiae as antigen. The contamination of monkeys in different open-air cages varied from 12.

[Morpho-immunologic phenotypes of T-cell non-Hodgkin's malignant lymphoma in baboons and their connection with with T-lymphotropic retrovirus STLV-1].

8 cytological types were detected among 58 cases of T-NHL of Papio hamadryas according to human updated Kiel classification (1988). 5 of them were of low grade (lymphocytic, prolymphocytic, T-zone, angioimmunoblastic, small cell pleomorphic) and 3-of high grade (medium and large cell pleomorphic, immunoblastic, large cell anaplastic CD30/Ki-1+). There are differences in a tumor structure and generalization in baboon and human lymphomas in spite of significant similarities.

[Large-cell anaplastic malignant CD30/Ki-1-positive T-type lymphoma, associated with STLV-1 virus (baboon malignant lymphoma model].

Large-cell anaplastic malignant lymphoma of T-type (T-LCAL) as an original form among human non-Hodgkin's malignant lymphomas in Updated Kiel Classification (1988) was defined. In human pathology T-LCAL presents a considerable diagnostic difficulties and is not associated with HTLV except some cutaneous forms. Comparative examination was carried out in 5 cases of baboon T-LCAL as a part of long-term investigation of the lymphoma outbreak in Sukhumi monkey stock.

Morphological characteristics of malignant T-cell lymphomas in baboons.

Fifteen cases of generalized peripheral T-cell non-Hodgkin's lymphoma in baboons were phenotyped immunologically and morphologically. Using the updated Kiel classification the cases included low-grade and high-grade lymphomas and low-grade lymphomas that had transformed into high-grade lymphomas. In the low-grade group there were seven cases of lymphocytic type, partly corresponding to chronic lymphocytic leukaemia of T type and to T-zone lymphoma in man.

Baboon T cell lymphomas expressing the B cell-associated surface proteins CD40 and Bgp95.

Papio hamadryas baboons in the Sukhumi colony develop enzootic outbreaks of malignant lymphomas with an incidence of about 1.5% per year among adults of the high-risk stock. We investigated the surface phenotypes of cells from normal and lymphomatous animals using antibodies against human lymphocyte antigens.

[Comparative study of lymph node gangliosides and blood serum of normal and T-lymphotrophic baboons].

The gangliosides from the lymph nodes and blood sera of normal and T-lymphomic baboons were studied. In lymph nodes the major gangliosides were identified as GM3 and GD3, those in blood sera--as GM3, GM1 and GD3. Gangliosides GM3 and GD3 contained N-acetyl as well as N-glycoloyl neuraminic acids.

[Non-Hodgkin's malignant lymphomas in primates (immunodiagnostic principles in T-cell lymphomas)].

Morphological and immunological phenotyping was carried out on 20 cases of peripheral baboon T-cell non-Hodgkin's malignant lymphomas (NHL) observed in a Sukhumi monkey colony. Morphologically, the lymphomas were prolymphocytic, immunoblastic, or rarely lymphoblastic types. For immunophenotyping studies, the panel of 29 monoclonal antibodies (MAb) to human or mouse leukocytic antigens were used (18 CD clusters).

[Modelling of malignant lymphoma in rabbits using primate oncogenic viruses. Preliminary report].

Inoculation of rabbits with permanent B-cell line cultures obtained from Stumptailed Macaques M. arctoides (MAL) which contain lymphotropic herpesvirus and C-type particles has led to the development of generalized lymphomas. The lymphoma cells had rabbit karyotype and did not contain surface an cytoplasmic immunoglobulins.

[B-cell and T-cell immunoblastic lymphomas of baboons].

The results of morphological study of B- and T-cellular immunoblast baboon lymphomas are presented. Differences are found both in the structure of tumour cells and in the histostructure of tumours as a whole. The tumor cells of B-immunoblastic lymphomas expressed mainly IgM.

Search for CEA-like molecules in polymorphonuclear leukocytes of non-human primates using monoclonal antibodies.

The monoclonal anti-CEA antibody ZIK-A42-A/C1 which reacts with NCA of human polymorphonuclear leukocytes was found to bind also to polymorphonuclear blood leukocytes of the following non-human primates tested: hamadryas baboon (Papio hamadryas), stump-tailed monkey (Macaca arctoides), pig-tailed monkey (Macaca nemestrina), and rhesus monkey (Macaca mulata). No binding was observed to mononuclear blood leukocytes. It was concluded that non-human primates contain CEA-like substances in their polymorphonuclear leukocytes as humans do and that these substances carry some identical epitopes.

Suppression of human lymphocyte mitogen response by retroviruses of type D. II. Non-activity of Mason-Pfizer monkey virus versus activity of human cell line derived virus PMFV.

In contrast to the human cell line derived type D retrovirus PMFV, the Mason-Pfizer monkey virus (MPMV) does not suppress the mitogen response of normal human lymphocytes. Both viruses have been propagated on the same cell lines and purified by the same methods. MPMV did not contain a factor able to abolish PMFV-induced suppression of the mitogen response.

[Leukemogenesis and bone marrow hematopoietic disorders in baboons with immunoblastic malignant lymphomas of B- and T-cell types].

Leukemization in baboon B- and T-cell immunoblastomas was investigated. Immunologic identification of tumor was carried out using standard immunological and cytochemical tests, e. g.

[Etiological aspects of leukemias in primates including man].

Attempts have been made to induce viral leukemia in monkeys (Papio hamadryas and Macaca arctoides) by inoculating them with blood from humans with different types of leukemias. In hamadryas baboons, the disease spread horizontally. By today 218 P.

Immunological investigation of the prelymphoma period in baboons of the Sukhumi monkey stock.

In the "pre-lymphoma" period, some high-risk baboons develop changes in their immunity which is expressed, in particular, in the imbalnace of T-lymphocyte subpopulations, that is, increase in the number of cells of T gamma subpopulation which are carriers of suppressive functions in man. As a reflection of immunological disturbances, increase in the level of antibodies against HVP, apparently as a result of HVP activation, is observed in baboons in the "pre-lymphoma" period.

[Study of virus-associated lymphomas in primates using a model of malignant lymphoma of the baboon].

An outbreak of malignant lymphoma was registered in a population of hamadryas baboons. Animals inoculated with blood from patients with various forms of leukemia were planted into the stock 10 years ago. 218 out of 3219 animals have already died from lymphoma.

[Macrophage electrophoretic mobility inhibition test (MET) in immunological diagnosis of malignant lymphomas in Papio baboons].

Reactivity of lymphocytes from P. hamadryas monkeys carrying malignant lymphomas, directed against soluble antigens from allogeneic lymphomas, was investigated, using macrophage electrophoretic mobility (MEM) test. Twenty-three monkeys have been examined.