Experimental and molecular modelling demonstration of effective DNA and protein binding as well as anticancer potential of two mononuclear Cu(II) and Co(II) complexes with isothiocyanate and azide as anionic residues.

In the present study, one mononuclear Cu(II) [CuL(SCN)] (1) and one mononuclear Co(II) [CoLN] (2) complexes, with a Schiff base ligand (HL) formed by condensation of 2-picolylamine and salicylaldehyde, have been successfully developed and structurally characterized. The square planer geometry of both complexes is fulfilled by the coordination of one deprotonated ligand and one ancillary ligand SCN(1) or N(2) to the metal centre. Binding affinities of both complexes with deoxyribonucleic acid (DNA) and human serum albumin (HSA) are investigated using several biophysical and spectroscopic techniques.

A multi-spectroscopic and molecular docking approach for DNA/protein binding study and cell viability assay of first-time reported pendent azide bearing Cu(II)-quercetin and dicyanamide bearing Zn(II)-quercetin complexes.

In the current study, one new quercetin-based Zn(II) complex [Zn(Qr)(CNNCN)(HO)] (Complex ) which is developed by condensation of quercetin with ZnCl in the presence of NaN(CN) and Cu(II) complex [Cu(Qr)N(CHOH)(HO)] (complex ) which is developed by the condensation reaction of quercetin and CuCl in presence of NaN are thoroughly examined in relation to their use in biomedicine. The results of several spectroscopic studied confirm the structure of both the complexes and the Density Functional Theory (DFT) study helps to optimize the structure of complex 1 and 2. After completion of the identification process, DNA and Human Serum Albumin (HSA) binding efficacy of both the investigated complexes are performed by implementing a long range of biophysical studies and a thorough analysis of the results unveils that complex has better interaction efficacy with the macromolecules than complex .

DNA/Protein Binding and Apoptotic-Induced Anticancer Property of a First Time Reported Quercetin-Iron(III) Complex Having a Secondary Anionic Residue: A Combined Experimental and Theoretical Approach.

A new quercetin-based iron(III) cationic complex [Fe()Cl(HO)(MeO)] (complex ) is created in the current study by condensation of quercetin with ferric chloride in the presence of EtN. Comprehensive spectroscopic analysis and conductometric measurement are used to pinpoint complex . The generated complex's +3-oxidation state has been verified by electron paramagnetic resonance (EPR) research.

Solvent-regulated fluorescence off-on signaling of Ni(II) and Zn(II) with the formation of two mononuclear complexes with an ATP detection ability by Zn(II) assembly.

The current study shows that Schiff base HL, ()-2,4-dibromo-6-(((piperidin-2-ylmethyl)imino)methyl)phenol, can be used successfully as a selective chemosensor for Zn(II) and Ni(II) among several competing cations in purely aqueous and semi-aqueous media. Under UV light in methanol-water (9 : 1) HEPES buffer, the receptor gives its response by changing its color to cyan color in the presence of Zn(II) and to bluish cyan color in the presence of Ni(II). Surprisingly, the chemosensor can only reliably identify Zn(II) in a hundred percent aqueous medium by changing its color to light yellow.

Response of Ancillary Azide Ligand in Designing a 1D Copper(II) Polymeric Complex along with the Introduction of High DNA- and HAS-Binding Efficacy, Leading to Impressive Anticancer Activity: A Compact Experimental and Theoretical Approach.

A new versatile azide-bridged polymeric Cu(II) complex, namely, [Cu(L)(μ-N)] (), was synthesized utilizing an N,N,O-donor piperidine-based Schiff base ligand ()-4-bromo-2-((2-(-1-yl)imino)methyl)phenol (), obtained the condensation reaction of 1-(2-aminoethyl) piperidine and 5-bromo salicylaldehyde. The single-crystal X-ray diffraction analysis reveals that complex consists of an end-to-end azido-bridged polymeric network, which is further rationalized with the help of a density functional theory (DFT) study. After routine characterization with a range of physicochemical studies, complex is exploited to evaluate its biomedical potential.

Structure and biological properties of exopolysaccharide isolated from Citrobacter freundii.

This study aimed to investigate the molecular characterization, antioxidant activity in vitro, cytotoxicity study of an exopolysaccharide isolated from Citrobacter freundii. Firstly, the culture conditions were standardized by the Design of experiments (DoE) based approach, and the final yield of thecrude exopolysaccharide was optimized at 2568 ± 169 mg L. One large fraction of exopolysaccharide was obtained from the culture filtrate by size exclusion chromatography and molecular characteristics were studied.

Structural Characterization of an Exopolysaccharide Isolated from Enterococcus faecalis, and Study on its Antioxidant Activity, and Cytotoxicity Against HeLa Cells.

An exopolysaccharide (EPS-I) having the molecular weight ~ 2.6 × 10 Da, was isolated from a Zinc resistant strain of Enterococcus faecalis from costal area. The exopolysaccharide consists of D-mannose, D-glucose, and L-fucose in molar ratio of 9:4:1.

Green synthesis, characterization, antimicrobial and cytotoxic effect of silver nanoparticles using arabinoxylan isolated from Kalmegh.

A green synthesis of silver nanoparticles was synthesized by AgNO with arabinoxylan, isolated from green stem of Andrographis paniculata (Kalmegh). The synthesized Ag NPs-arabinoxylan conjugates were characterized by UV-vis spectroscopy, FE-SEM, TEM, XRD, TGA, EDX, and Zeta potential experiments. The Ag NPs formation was established by the surface plasmon resonance band ~410.

Structural studies of a water insoluble β-glucan from Pleurotus djamor and its cytotoxic effect against PA1, ovarian carcinoma cells.

A water insoluble β-glucan (PS), with molecular mass ∼9.16 × 10 Da was isolated from the 4% alkaline extract of an edible mushroom, Pleurotus djamor and found to consist of (1→3)-β-d-glucopyranosyl moiety. The structure of the PS was elucidated on the basis of total hydrolysis, methylation analysis, periodate oxidation, and NMR experiments (H, C, DQF-COSY, DEPT-135, and HSQC).

Detection of Somatic Mutation in Exon 12 of DNA Polymerase β in Ovarian Cancer Tissue Samples.

Background: DNA polymerase β (pol β) is a key enzyme of base excision repair pathway. It is a 1-kb gene consisting of 14 exons. Its catalytic part lies between exon 8 and exon 14.