Two spheres translating in tandem through a colloidal suspension.

Using laser tweezers, two colloidal particles are held parallel to a uniformly flowing suspension of similarly sized bath particles at an effective volume fraction ϕ(eff)=0.41. The local deformation in the bath suspension is imaged by confocal microscopy, and, concurrently, the drag forces exerted on both the leading and the trailing probe particles are measured as a function of probe separation and velocity.

Single-molecule resolution of protein dynamics on polymeric membrane surfaces: the roles of spatial and population heterogeneity.

Although polymeric membranes are widely used in the purification of protein pharmaceuticals, interactions between biomolecules and membrane surfaces can lead to reduced membrane performance and damage to the product. In this study, single-molecule fluorescence microscopy provided direct observation of bovine serum albumin (BSA) and human monoclonal antibody (IgG) dynamics at the interface between aqueous buffer and polymeric membrane materials including regenerated cellulose and unmodified poly(ether sulfone) (PES) blended with either polyvinylpyrrolidone (PVP), polyvinyl acetate-co-polyvinylpyrrolidone (PVAc-PVP), or polyethylene glycol methacrylate (PEGM) before casting. These polymer surfaces were compared with model surfaces composed of hydrophilic bare fused silica and hydrophobic trimethylsilane-coated fused silica.

Line tension and line activity in mixed monolayers composed of aliphatic and terphenyl-containing surfactants.

Custom-designed surfactants, known as "linactants", have the ability to reduce the line tension between coexisting phases within mixed monolayers of chemically dissimilar compounds at the air-water interface. Thus far, linactants have been successfully identified for only one type of chemical dissimilarity, involving mixed monolayers of hydrocarbon and fluorocarbon surfactants. In the present work, we have pursued a more general interpretation of linactant compounds by extending the concept to a new system that is comprised of a mixture of aliphatic (pentadecanoic acid) and aromatic (p-terphenyl carboxylic acid) compounds.