sp. nov., isolated from the gut of an olive flounder .

A Gram-stain-negative, facultatively anaerobic, flagellated and coccoid, ovoid or rod-shaped bacterial strain, NIFS-20-8, was isolated from the intestine of an olive flounder () from the East Sea, Republic of Korea. The neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain NIFS-20-8 fell within the clade comprising the type strains of species. Strain NIFS-20-8 exhibited 16S rRNA gene sequence similarities of 97.

gen. nov., sp. nov., isolated from stalked sea squirt .

A Gram-stain-negative, strictly aerobic, non-motile and rod-shaped bacterial strain, MYP1-1, was isolated from the intestine of a stalked sea squirt () of the South Sea in the Republic of Korea. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain MYP1-1 clustered with the type strains of species and . Strain MYP1-1 exhibited 16S rRNA gene sequence similarity values of 97.

Should We Delay Urodynamic Study When Patients With Spinal Cord Injury Have Asymptomatic Pyuria?

Objective: To assess the incidence of urinary tract infection (UTI) with post-urodynamic study (post-UDS) in patients with spinal cord injury (SCI) and study its relationship with pre-UDS pyuria.

Methods: Patients with SCI who were hospitalized and underwent UDS during a 4-year period were reviewed. Patients with pre-test lower urinary tract symptoms were excluded.

sp. nov., a gammaproteobacterium isolated from seawater.

A Gram-stain-negative, aerobic, non-spore-forming, non-motile and ovoid or rod-shaped bacterial strain, MYP5, was isolated from seawater in Jeju island of South Korea. MYP5 grew optimally at 30-35 °C and in the presence of 2.0 % (w/v) NaCl.

gen. nov., sp. nov., isolated from seawater.

A Gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, designated MYP11, was isolated from seawater around Jeju island, Republic of Korea and identified by polyphasic taxonomic study. A neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain MYP11 joined the cluster comprising the type strains of , and , showing 16S rRNA gene sequence similarities of 96.3-96.

sp. nov., isolated from an intestine of Nile tilapia ().

A Gram-stain-negative, aerobic, non-motile and ovoid or rod-shaped bacterial strain, MRS2, was isolated from an intestine of Nile tilapia () collected from the Republic of Korea. Strain MRS2 grew optimally at 30 °C and in the presence of 0-2.0 % (w/v) NaCl.

Paracoccus alimentarius sp. nov., isolated from a Korean foodstuff, salted pollack.

A Gram-stain-negative, aerobic, non-motile and coccoid or ovoid bacterial strain, designated LB2, was isolated from a Korean foodstuff, salted pollack. Strain LB2 grew optimally at 25-30 °C, at pH 7.0-8.

Bizionia berychis sp. nov., isolated from intestinal tract of a splendid alfonsino (Beryx splendens).

A Gram-stain-negative, non-motile, aerobic and rod-shaped bacterial strain, designated RA3-3-1, was isolated from splendid alfonsino (Beryxsplendens) collected from the North Pacific Ocean. Strain RA3-3-1 grew optimally at 25 °C, at pH 7.0-8.

Tenacibaculum haliotis sp. nov., isolated from the gut of an abalone Haliotis discus hannai.

A Gram-stain-negative, non-flagellated, gliding, non-spore-forming bacterial strain, designated RA3-2T, was isolated from the gut of an abalone (Haliotis discus hannai) collected from the sea around Jeju island, South Korea, and subjected to a polyphasic taxonomic study. RA3-2T grew optimally at 20 °C and in the presence of 2.0-3.

Polaribacter haliotis sp. nov., isolated from the gut of abalone Haliotis discus hannai.

A Gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, designated RA4-7T, was isolated from the gut of an abalone (Haliotis discus hannai) collected from the sea around Jeju island, South Korea, and subjected to a polyphasic taxonomic study. RA4-7T grew optimally at 25 °C, at pH 7.0-7.

Colwellia mytili sp. nov., isolated from mussel Mytilus edulis.

A Gram-stain-negative, aerobic, motile and rod-shaped bacterial strain, designated RA2-7T, was isolated from a mussel (Mytilus edulis) collected from the South Sea, South Korea, and subjected to a taxonomic study using a polyphasic approach. Strain RA2-7T grew optimally at 20 °C, at pH 7.0-8.

Tenacibaculum ascidiaceicola sp. nov., isolated from the golden sea squirt Halocynthia aurantium.

A Gram-stain-negative, non-flagellated, non-spore-forming bacterial strain motile by gliding, designated RSS1-6T, was isolated from a golden sea squirt Halocynthia aurantium and its taxonomic position was investigated by using a polyphasic approach. Strain RSS1-6T grew optimally at 30-37 °C and in the presence of 1.0-4.

Octadecabacter ascidiaceicola sp. nov., isolated from a sea squirt (Halocynthia roretzi).

A Gram-stain-negative, non-spore-forming, non-flagellated and coccoid, ovoid or rod-shaped bacterial strain, RA1-3T, was isolated from a sea squirt (Halocynthia roretzi) collected from the South Sea, South Korea, and subjected to a taxonomic study using a polyphasic approach. Strain RA1-3T grew optimally at 25 °C, at pH 7.0-8.

A novel esterase from Paenibacillus sp. PBS-2 is a new member of the β-lactamase belonging to the family VIII lipases/esterases.

Screening of a gene library from Paenibacillus sp. PBS-2 generated in Escherichia coli led to the identification of a clone with lipolytic activity. Sequence analysis showed an open reading frame encoding a polypeptide of 378 amino acid residues with a predicted molecular mass of 42 kDa.

Molecular and functional characterizations of a Kunitz-type serine protease inhibitor FcKuSPI of the shrimp Fenneropenaeus chinensis.

Serine proteinase inhibitors play important and diverse roles in biological processes such as coagulation, defense mechanisms, and immune responses. Here, we identified and characterized a Kunitz-type proteinase inhibitor, designated FcKuSPI, of the BPTI/Kunitz family of serine proteinase inhibitors from the hemocyte cDNA library of the shrimp Fenneropenaeus chinensis. The deduced amino acid sequence of FcKuSPI comprises 80 residues with a putative signal peptide of 15 amino acids.

A novel cold-adapted esterase from Salinisphaera sp. P7-4: gene cloning, overproduction, and characterization.

Salinisphaera sp. P7-4 was isolated from the intestine of silver whiting, Sillago japonicas caught in the Pacific Ocean, and the esterase gene was cloned using the shotgun method. The amino acid sequence deduced from the nucleotide sequence (951 bp) corresponded to a protein of 316 amino acid residues with a molecular weight of 34,443.

Purification and biochemical characterization of a 17 kDa fibrinolytic enzyme from Schizophyllum commune.

A fibrinolytic enzyme of the mushroom, Schizophyllum commune was purified with chromatographic methods, including a DEAE-Sephadex A-50 ion-exchange column and gel filtrations with Sephadex G-75 and Sephadex G-50 columns. The analysis of fibrin-zymography and SDS-PAGE showed that the enzyme was a monomeric subunit that was estimated to be approximately 17 kDa in size. The fibrinolytic activity of the enzyme in plasminogen-rich and plasminogen-free fibrin plates was 1.

Immediate and delayed lateral ridge expansion technique in the atrophic posterior mandibular ridge.

Purpose: The lateral ridge expansion technique is used to expand the narrow edentulous ridge for implant placement. The staged approach can be used to split the mandibular ridge to decrease the risk of malfracture during osteotomy. The present study reports the clinical results of a surgical technique that expands a narrow mandibular ridge using an immediate and a delayed lateral expansion technique.

Purification, characterization, and gene cloning of glucose-1-phosphatase from Citrobacter braakii.

Citrobacter braakii produced an intracellular acid glucose phosphatase (AgpC) which was purified 986 fold to homogeneity with the specific activity of 286 units/mg. AgpC hydrolyzed a wide variety of phosphorylated compounds with high activity for glucose-1-phosphate and glucose-6-phosphate. The optimum pH and temperature for the enzyme activity was pH 5.

Soluble expression of recombinant olive flounder hepcidin I using a novel secretion enhancer.

Expression of olive flounder hepcidin I (HepI) fused with truncated OmpA signal peptides (OmpASP(tr)) as directional signals does not produce soluble fusion proteins. However, by inserting amino acid segments (xxx) varying in pI and hydrophobicity/hydrophilicity into a leader sequence containing a truncated OmpASP (OmpASP(tr)) and a factor Xa cleavage site (Xa) [OmpASP(tr)|(xxx)|Xa], we were able in some cases to express soluble recombinant HepI. Soluble expression of the recombinant protein strongly correlated with (xxx) insertions of high pI and hydrophilicity.

Development and characterization of membrane surface display system using molecular chaperon, prsA, of Bacillus subtilis.

We report a new membrane surface display system based on molecular chaperon, prsA, of Bacillus subtilis. Clostridium thermocellum cellulase, celA, was fused to C-terminal end of PrsA. Cellulase activity of B.

The effects of ftsZ mutation on the production of recombinant protein in Bacillus subtilis.

In this paper, the possibility of using a mutation of ftsZ as a pseudo-spore mutant is investigated. ftsZ, which is essential for cell division and sporulation of Bacillus subtilis, was placed under the spac promoter, which is inducible with isopropyl thiogalactose (IPTG). Cell growth of the ftsZ mutant and its beta-galactosidase activity under the aprE promoter were compared with the wild type.