Rapid determination of vitamin D₃ in milk-based infant formulas by liquid chromatography-tandem mass spectrometry.

A rapid and simple sample preparation method for vitamin D3 (cholecalciferol) was developed for emulsified dairy products such as milk-based infant formulas. A sample was mixed in a 50 mL centrifuge tube with the same amount of water and isopropyl alcohol to achieve chemical extraction. Ammonium sulfate was used to induce phase separation.

Rapid determination of cholesterol in milk containing emulsified foods.

In this study, a rapid and easy sample preparation method that involved no-heating saponification and dSPE (dispersive solid phase extraction) clean-up was developed to determine the level of cholesterol in milk containing emulsified foods (infant formula, baby food, cheese). The developed method utilised high performance liquid chromatography with an ultraviolet detector (HPLC-UVD) as a separation instrument. The optimum extraction conditions were determined as 10 mL isopropyl alcohol with 8.

A novel sample preparation method using rapid nonheated saponification method for the determination of cholesterol in emulsified foods.

Unlabelled: In this study, nonheated saponification was employed as a novel, rapid, and easy sample preparation method for the determination of cholesterol in emulsified foods. Cholesterol content was analyzed using gas chromatography with a flame ionization detector (GC-FID). The cholesterol extraction method was optimized for maximum recovery from baby food and infant formula.

Determination of pesticide residues in milk using a QuEChERS-based method developed by response surface methodology.

Response surface methodology (RSM) was employed to optimise a rapid and easy sample preparation method for the determination of 14 different pesticide residues in milk. The recovery of each pesticide was investigated by a QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe)-based method with respect to 3 variables, the amounts of sodium acetate (Na Acetate), primary secondary amine (PSA), and octadecylsilane (C18). After optimisation, the maximum predicted recovery was the 99.