Publications by authors named "In-Hui Kim"
Increased survival of the honey bee Apis mellifera infected with the microsporidian Nosema ceranae by effective gene silencing.
Arch Insect Biochem Physiol
December 2020
This study examined the control of nosemosis caused by Nosema ceranae, one of the hard-to-control diseases of honey bees, using RNA interference (RNAi) technology. Double-stranded RNA (dsRNA) for RNAi application targeted the mitosome-related genes of N. ceranae.
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- The study focuses on improving the purification process for spores of an intracellular fungal parasite that harms honey bees and increases their vulnerability to other pathogens.
- By incorporating 2 mm beads and enzymatic treatments with collagenase and trypsin, researchers significantly increased the yield of spores extracted from the honey bee midgut.
- The optimal treatment duration was found to be one hour, and a simple filtration method effectively removed cell debris, making this technique useful for purifying other similar fungal spores as well.