Medullins A-H, Sesquiterpenes from , and Their Cellular Signaling Mechanism in HDF Cells.

A chemical investigation of an ethyl acetate-soluble layer in the culture broth of resulted in the isolation of eight novel sesquiterpenes conjugated Gly (), l-Val (), l-Ala (), l-Tyr (), l-Thr (), l-Ile (), l-Leu (), and l-Phe (). Elucidation of their structures was performed through comprehensive spectroscopic analysis. The absolute configuration of the sesquiterpene skeleton was ascertained using modified Mosher's methods.

Formanilides from the culture broth of Perenniporia fraxinea.

A new formanilide dimer, fraxinin (1), and three known formanilides (2‒4) were isolated from the culture broth of Perenniporia fraxinea using silica gel and Sephadex LH-20 column chromatographies, medium-pressure liquid chromatography (MPLC), and preparative HPLC. The structures of these compounds were determined by spectroscopic methods, such as NMR and mass analysis, and by comparison of the spectra with previously reported data. The free radical scavenging activities of the isolated compounds were assessed using 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals.

Pullusurfactins A‒C, new biosurfactants produced by Aureobasidium pullulans A11231-1-58 from Chrysanthemum boreale Makino.

Biosurfactants have found widespread use across multiple industrial fields, including medicine, food, cosmetics, detergents, pulp, and paper, as well as the degradation of oil and fat. The culture broth of Aureobasidium pullulans A11231-1-58 isolated from flowers of Chrysanthemum boreale Makino exhibited potent surfactant activity. Surfactant activity-guided fractionation led to the isolation of three new biosurfactants, pullusurfactins A‒C (1‒3).

Chemical constituents of the culture broth of Dentipellis fragilis and their anti-inflammatory activities.

Seven undescribed compounds, dentipellinones A‒D (1, 2, 5, and 6), dentipellinol (3), methoxyerinaceolactone B (4), and erinaceolactomer A (7), were isolated from the culture broth of Dentipellis fragilis. Chemical structures of these isolated compounds were determined by analyses of 1D and 2D-NMR and MS data in comparison with data reported in the literature. Absolute configurations of 1‒7 were also determined by Electronic Circular Dichroism calculations.

Identification of Loliolide with Anti-Aging Properties from JD052.

Herein, different extracts of JD052, a green microalga, were evaluated in vitro as a potential anti-aging bioagent. Although post-treatment of microalgal culture with either UV irradiation or high light illumination did not lead to a substantial difference in the effectiveness of microalgal extracts as a potential anti-UV agent, the results indicated the presence of a highly potent compound in ethyl acetate extract with more than 20% increase in the cellular viability of normal human dermal fibroblasts (nHDFs) compared with the negative control amended with DMSO. The subsequent fractionation of the ethyl acetate extract led to two bioactive fractions with high anti-UV property; one of the fractions was further separated down to a single compound.

p-Terphenyl glucosides from the culture broth of Phlebiopsis castanea.

A new p-terphenyl derivative, hydroxystrepantibin D (1), was isolated along with two known p-terphenyls (2 and 3) from the culture broth of Phlebiopsis castanea. These compounds were isolated using silica gel column chromatography, reversed-phase medium-pressure liquid chromatography, Sephadex LH-20 column chromatography, and preparative HPLC. Their structures were determined based on spectroscopic methods.

Two Novel Biosurfactants Produced by Aureobasidium pullulans A11211-4-57 from a Fleabane, Erigeron annus (L.) pers.

Biosurfactants have been widely used in various industrial fields including medicine, food, cosmetics, detergent, pulp and paper, and oil and fat degradation. The culture broth of Aureobasidium pullulans A11211-4-57 using glucose as carbon source exhibited potent surfactant activity. The culture broth was separated by column chromatographies using ODS, silica gel, and Sephadex LH-20 resins, consecutively, to provide two biosurfactants.

Chemical Constituents of the Culture Broth of .

In our ongoing search for new secondary metabolites from fungal strains, one novel compound () and nine known compounds () were isolated from the EtOAc-soluble layer of the culture broth of . The culture broth of was extracted in acetone and fractionated by solvent partition; column chromatography using silica gel, Sephadex LH-20, and Sephadex G-10; MPLC; and HPLC. The structures of isolated compounds were elucidated by one- and two-dimensional NMR and LC-ESI-mass measurements.

Daldinans D‒G, new isoindolinone antioxidants isolated from the ascomycete Daldinia concentrica.

Four new isoindolinone derivatives, daldinans D‒G (3‒6), together with two known compounds, daldinans A and B (1 and 2), were isolated from the stroma of the ascomycete Daldinia concentrica. Chemical structures of the isolated compounds were determined by spectroscopic methods. All of these compounds exhibited antioxidant activities with IC values of 3.

New antioxidants from the culture broth of Coprinopsis echinospora.

During the search for natural antioxidants from fungal metabolites, three new sesquiterpene derivatives (1-3) have been isolated from the culture broth of Coprinopsis echinospora. Their structures were determined by spectroscopic methods, mainly NMR and mass spectrometric analyses. These compounds exhibited antioxidant activity with IC values in the range of 34.

Reducing tumor invasiveness by ramucirumab and TGF-β receptor kinase inhibitor in a diffuse-type gastric cancer patient-derived cell model.

Background: Diffuse-type gastric cancer (GC) is known to be more aggressive and relatively resistant to conventional chemotherapy. Hence, more optimized treatment strategy is urgently needed in diffuse-type GC.

Methods: Using a panel of 10 GC cell lines and 3 GC patient-derived cells (PDCs), we identified cell lines with high EMTness which is a distinct feature for diffuse-type GC.

Consoramides A-C, New Zwitterionic Alkaloids from the Fungus .

In our ongoing search for new secondary metabolites from fungi, a basidiomycete fungus was selected for mycochemical investigation, and three new zwitterionic alkaloids (-) and five known compounds (-) were isolated from the culture broth (16 l) of . The culture filtrate was fractionated by a series of column chromatography including Diaion HP-20, silica gel, and Sephadex LH-20, Sep-Pak C cartridge, medium pressure liquid chromatography (MPLC), and high pressure liquid chromatography (HPLC) to yield eight compounds (-). The structures of the isolated compounds were elucidated by the interpretation of nuclear magnetic resonance (NMR) spectra and high-resolution mass spectrometry (HR-MS).

Dentipellin, a new antibiotic from culture broth of Dentipellis fragilis.

In our effort to find antimicrobial agents from higher fungi, we isolated a new compound, dentipellin (1), along with three known glycosylated diterpenes, erinacines A-C (2-4) from culture broth of Dentipellis fragilis. Their chemical structures were determined by spectroscopic methods including NMR and mass measurements. These compounds exhibited weak antibacterial and antifungal activities.

TPK1 as a predictive marker for the anti-tumour effects of simvastatin in gastric cancer.

The potential anti-tumour role of statins has been reported in various cancer types, including gastric cancer (GC). However, there are no biomarkers to identify patients who may benefit most from this treatment. We tested the effects of statins on 8 GC cell lines.

Spirobenzofuran (SBF): An isolate from Acremonium sp. HKI 0230 and Coprinus echinosporus suppresses inflammation in RAW 264.7 cells.

Inflammation is an aggregate of different pathologic responses in body that leads to life threatening conditions if not combated at early stages. A variety of chemical medications from low quality to high quality are available in market for treatment of inflammation. However the side effects posed by these medications cannot be ignored.

Rhizophins A and B, new sesquiterpenes from the culture broth of Coprinus rhizophorus.

Two new sesquiterpenes (1 and 2) together with one known compound (3) were isolated from the culture broth of Coprinus rhizophorus. Chemical structures of these compounds were deduced by spectroscopic methods. Compound 1 exhibited free-radical-scavenging activity against the 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical.

Oregonensins A and B, new meroterpenoids from the culture broth of Ganoderma oregonense and their antioxidant activity.

Two new meroterpene derivatives (1 and 2), together with three known compounds (3-5) were isolated from the culture broth of fungus Ganoderma oregonense. Chemical structures of compounds 1-5 were determined using spectroscopic methods. All compounds were tested for 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging efficacy.

Neuraminidase Inhibitors from the Fruiting Body of .

Neuraminidase (NA) cleaves the glycosidic bond linkages of sialic acids to release the mature virions from infected cells and has been an attractive therapeutic target for anti-influenza agents. In our ongoing investigation of NA inhibitors in mushroom extracts, we found that the extract the fruiting body of potently inhibited neuraminidase. The fruiting bodies of .

Neutralizing antibody to FGFR2 can act as a selective biomarker and potential therapeutic agent for gastric cancer with FGFR2 amplification.

() amplification has been reported in 5-10% of gastric cancer (GC) and is associated with poor prognosis. In this study, we characterized the anti-tumor effect of PRO-007, a newly developed recombinant monoclonal antibody that targets FGFR2, in GC cell lines KATO III (with amplification) and NCI-N87 (without amplification). Validation was performed in parallel using two patient-derived tumor cells (PDCs) from patients with GC.