An effective method for improving outcomes in patients with a fertilization defect.

The effect of artificial oocyte activation (AOA) with a calcium ionophore on intracytoplasmic morphologically selected sperm injection (IMSI) was examined in patients with histories of repeated failed implantation attempts. Four singleton pregnancies and one twin pregnancy were obtained after embryos transfer (5/14, 35.7%).

Analysis of clinical outcomes with respect to spermatozoan origin after artificial oocyte activation with a calcium ionophore.

Purpose: Fertilization failures have occurred repeatedly in reproductive centers after intracytoplasmic sperm injection (ICSI) and artificial oocyte activation (AOA) has been used to prevent it. This study was performed to investigate whether spermatozoan origin influences clinical outcomes of AOA with a calcium ionophore.

Methods: A total of 185 ICSI cycles with a history of no or low fertilization was included in this retrospective study.