Objectives: CD34+ cells and colony forming unit-granulocyte and macrophage (CFU-GM) from human bone marrow were used to investigate the role of Fas/FasL system in the regulation of myelopoiesis.
Methods: Fas and FasL expression in CD34+ cells and in day 14 CFU-GM were measured by RT-PCR and immunofluorescence respectively. The functional assays for the CFU-GM were measured by a standard colony assay and the proliferative capacity of CFU-GM was measured by replating the primary colony and observing the secondary colony formation.
Objective: Neutrophil alloantigens are responsible for a number of immune clinical disorders such as neonatal alloimmune neutropenia, febrile transfusion reaction and transfusion related acute lung injury. Recent progress enabled typing of neutrophil antigens by deoxyribose nucleic acid based techniques. In this study we report for the first time the frequency distribution of human neutrophil antigen system-one in Saudis.
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