Application of High-Resolution Melting PCR to Detect the Genomic Fungal ITS 2 Region.

Background: Invasive fungal infections (IFIs) are a main cause of morbidity and mortality. High-resolution melting polymerase chain reaction (HRM PCR) is promising for the identification of fungal species via the detection of internal transcribed spacer 2 (ITS2).

Objectives: To assess the sensitivity and specificity of HRM PCR in diagnosing IFIs, compared with blood culture.

Genotypic Identification of AmpC β-Lactamases Production in Gram-Negative Bacilli Isolates.

Background: AmpC type β-lactamases are commonly isolated from extended-spectrum Cephalosporin-resistant Gram-negative bacteria. Also, resistance appeared in bacterial species not naturally producing AmpC enzymes. Therefore, a standard test for the detection of the plasmid-mediated AmpC enzyme and new breakpoints for extended spectrum Cephalosporins are urgently necessary.

Enteropathogenic Escherichia coli associated with diarrhea in children in Cairo, Egypt.

In this study we isolate and identify the Enteropathogenic Escherichia coli (EPEC) causing diarrhea in children less than five years in Cairo, Egypt, during different seasons. Children younger than five years with diarrhea, attending the Pediatric Gastroenterology Intensive Care Unit of the Cairo University Pediatric Hospital in one year period were our group of study. Our control group was age and sex matched concurrent healthy children.