Publications by authors named "Im Kwok"
Article Synopsis
- High-dimensional single-cell technologies have become essential for studying the immune system, but analyzing the resulting data is complex and computationally demanding.
- The authors introduce Cyclone, an analysis pipeline that simplifies and optimizes clustering and visualization of cytometry data across different techniques and biological contexts.
- Cyclone not only matches the accuracy of established immune cell identification methods but also helps discover new subsets of immune cells linked to specific biological characteristics, enhancing immunology research.
Article Synopsis
- High-dimensional single cell technologies have transformed immunology research, enabling scientists to study the immune system more effectively.
- Cyclone is a new analysis pipeline that integrates necessary tools for data processing, such as dimensionality reduction and clustering, making it easier to analyze complex cytometry data.
- Cyclone has been tested on various cytometry techniques and has been shown to accurately identify immune cell types, facilitating further research and discoveries in immunology.
Responsiveness to immune checkpoint blockade (ICB) therapy in cancer is currently predicted by disparate individual measures - with varying degrees of accuracy - including tumor mutation burden, tumor-infiltrating T cell densities, dendritic cell frequencies, and the expression of checkpoint ligands. We propose that many of these individual parameters are linked, forming two distinct 'reactive' immune archetypes - collections of cells and gene expression - in ICB-responsive patients. We hypothesize that these are 'seeds' of antitumor immunity and are supported by specific elements of the tumor microenvironment (TME) and by actions of the microbiome.
View Article and Find Full Text PDFUnlabelled: Individuals with SHANK3 mutations have severely impaired receptive and expressive language abilities. While brain responses are known to be abnormal in these individuals, the auditory cortex response to sound has remained largely understudied. In this study, we document the auditory cortex response to speech and non-speech sounds in the novel Shank3-deficient rat model.
View Article and Find Full Text PDFIndividuals with Rett syndrome have greatly impaired speech and language abilities. Auditory brainstem responses to sounds are normal, but cortical responses are highly abnormal. In this study, we used the novel rat Mecp2 knockout model of Rett syndrome to document the neural and behavioral processing of speech sounds.
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- Kids with autism often have trouble with talking and understanding speech.
- Giving lots of practice and help can make their speech better and help their brains respond to sounds.
- Researchers studied rats that were affected by a drug before they were born and found that with training, these rats got much better at hearing and recognizing different sounds.
Article Synopsis
- Fragile X syndrome is the most common inherited cause of intellectual disability and can also lead to autism.
- In a study with rats that have fragile X syndrome, researchers found that their brains did not process speech sounds as well as normal rats, even though they could still tell the sounds apart.
- The study suggests that problems in a specific part of the brain related to emotions could explain why people with fragile X syndrome have trouble with emotional control.
An LC/MS-based method is established for the differentiation and authentication of specimens and commercial samples of Panax ginseng (Oriental ginseng) and Panax quinquefolius (American ginseng). This method is based on the separation of ginsenosides present in the ginseng methanolic extracts using high-performance liquid chromatography (HPLC), followed by detection with electrospray mass spectrometry. Differentiation of ginsenosides is achieved through simultaneous detection of intact ginsenoside molecular ions and the ions of their characteristic thermal degradation products.
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