Optimization of MBRs through integrated modelling: A state of the art.

The optimization of integrated membrane bioreactors (MBRs) models is of paramount importance in view of reducing the costs, greenhouse gas emissions or enhancing the water quality. On this behalf, this paper, produced by the International Water Association (IWA) Task Group on Membrane modelling and control, reviews the current state-of-the-art regarding the control and optimization of integrated MBR models. Whether aerobic or anaerobic, such modelling allows the consideration of specific functioning conditions and optimization problems together with the estimation and monitoring of Performance Index (PIs).

A review on co-metabolic degradation of organic micropollutants during anaerobic digestion: Linkages between functional groups and digestion stages.

The emerging presence of organic micropollutants (OMPs) in water bodies produced by human activities is a source of growing concern due to their environmental and health issues. Biodegradation is a widely employed treatment method for OMPs in wastewater owing to its high efficiency and low operational cost. Compared to aerobic degradation, anaerobic degradation has numerous advantages, including energy efficiency and superior performance for certain recalcitrant compounds.

Corrigendum to 'Amyloid adhesin production in activated sludge is enhanced in lab-scale sequencing batch reactors: Feeding regime impacts microbial community and amyloid distribution' [Water Research X 17 (2022) 100162].

[This corrects the article DOI: 10.1016/j.wroa.

gen. nov., sp. nov., a thermophilic chain-elongating bacterium, producing -caproate from polymeric carbohydrates.

Strain MDTJ8 is a chain-elongating thermophilic bacterium isolated from a thermophilic acidogenic anaerobic digestor treating human waste while producing the high commodity chemical -caproate. The strain grows and produces formate, acetate, -butyrate, -caproate and lactate from mono-, di- and polymeric saccharides at 37-60 °C (optimum, 50-55 °C) and at pH 5.0-7.

Quantification of hydrolysis activity in a biological wastewater treatment context.

This paper reviews currently available methods for hydrolysis activity monitoring of the most commonly encountered enzyme categories in biological wastewater treatment. While highlighting the relevant methods for protein, lipid, carbohydrate, organic phosphate, and ester hydrolysis, the discussion of their pros and cons is predominantly aimed at revealing the relevance of the to-be-hydrolyzed substrates that are used in the methods. These "substrates" should mimic the proteins, lipids, or other polymers that are present in the wastewater and are in the reviewed methods (i) real substrates (i.

Amyloid adhesin production in activated sludge is enhanced in lab-scale sequencing batch reactors: Feeding regime impacts microbial community and amyloid distribution.

Amyloid adhesins are β-sheet-rich extracellular proteins thought to contribute to bioflocculation. They are present in activated sludge to varying extent. However, it remains unclear which operational conditions promote their production.

Isolation and characterization of a thermophilic chain elongating bacterium that produces the high commodity chemical n-caproate from polymeric carbohydrates.

A thermophilic chain elongating bacterium, strain MDTJ8, was isolated from a thermophilic acidogenic anaerobic digestor producing n-caproate from human waste, growing optimally at 50-55 °C and pH 6.5. 16S rRNA gene analysis suggests that MDTJ8 represents a new species/genus within a group currently composed of mesophilic chain elongators of the Oscillospiraceae family.

Integrated membrane bioreactors modelling: A review on new comprehensive modelling framework.

Integrated Membrane Bioreactor (MBR) models, combination of biological and physical models, have been representing powerful tools for the accomplishment of high environmental sustainability. This paper, produced by the International Water Association (IWA) Task Group on Membrane Modelling and Control, reviews the state-of-the-art, identifying gaps for future researches, and proposes a new integrated MBR modelling framework. In particular, the framework aims to guide researchers and managers in pursuing good performances of MBRs in terms of effluent quality, operating costs (such as membrane fouling, energy consumption due to aeration) and mitigation of greenhouse gas emissions.

A high-throughput assay to quantify protein hydrolysis in aerobic and anaerobic wastewater treatment processes.

Proteins, an important fraction of the organic matter in wastewater, typically enter a treatment facility as high molecular weight components. These components are degraded by extracellular protein hydrolytic enzymes, denoted as proteases. Adequate protein hydrolysis monitoring is crucial, since protein hydrolysis is often a rate-limiting step in wastewater treatment.

Improving anaerobic digestion via direct interspecies electron transfer requires development of suitable characterization methods.

Recent anaerobic digestion studies commonly attribute performance improvements (e.g. increased methane production, enhanced process stability, reduced startup times) to direct interspecies electron transfer (DIET), even though only indirect evidence of DIET is available and DIET alone does not explain enhanced performance in many cases.

Ultrasound assisted chrome tanning: Towards a clean leather production technology.

Nowadays, there is a growing demand for a cleaner, but still effective alternative for production processes like in the leather industry. Ultrasound (US) assisted processing of leather might be promising in this sense. In the present paper, the use of US in the conventional chrome tanning process has been studied at different pH, temperature, tanning time, chrome dose and US exposure time by exposing the skin before tanning and during tanning operation.

Impact of Tannery Effluent on the Self-purification Capacity and Biodiversity Level of a River.

The present study investigates the impact of tannery effluents on the self-purification capacity and the local macroinvertebrate community of one natural stream. As the concentration of chromium and sulfide increased from up- to downstream sites, the reduction of suspended solids, 5-days biological oxygen demand (BOD5), chemical oxygen demand and nitrification capacity decreased by 61 %, 21 %, 30 % and 74 %, respectively. Similarly, the share of Ephemeroptera, Plecoptera, and Trichoptera on the macroinvertebrate community decreased from 24 % to 0 %.

Addition of polyaluminiumchloride (PACl) to waste activated sludge to mitigate the negative effects of its sticky phase in dewatering-drying operations.

This paper presents a new application of polyaluminiumchloride (PACl) as a conditioner for waste activated sludge prior its dewatering and drying. It is demonstrated at lab scale with a shear test-based protocol that a dose ranging from 50 to 150 g PACl/kg MLSS (mixed liquor suspended solids) mitigates the stickiness of partially dried sludge with a dry solids content between 25 and 60 %DS (dry solids). E.

Identifying sustainable rehabilitation strategies for urban wastewater systems: a retrospective and interdisciplinary approach. Case study of Coronel Oviedo, Paraguay.

Many wastewater managers in developing countries struggle with the daily operation of urban wastewater systems. Technically well-designed wastewater collection and treatments are often degraded and/or not properly functioning. In this paper, a realistic rehabilitation strategy is developed for the urban wastewater system of Coronel Oviedo (Paraguay), in which the actual performance is unsatisfactory, as revealed by a detailed technical assessment, including water quantity and quality monitoring data.

Liver X receptors regulate cholesterol homeostasis in oligodendrocytes.

Cholesterol synthesis and transport in oligodendrocytes are essential for optimal myelination and remyelination in pathological conditions such as multiple sclerosis. However, little is known about cholesterol homeostasis in the myelin-forming oligodendrocytes. Liver X receptors (LXRs) are nuclear oxysterol receptors that regulate genes involved in cholesterol homeostasis and may therefore play an important role in de- and remyelination.

Simvastatin interferes with process outgrowth and branching of oligodendrocytes.

Statins have attracted interest as a treatment option for multiple sclerosis (MS) because of their pleiotropic antiinflammatory and immunomodulatory effects. However, contradictory results have been described when they are applied to oligodendrocytes (OLGs), the cell type predominantly affected in MS. In this study we focus on the in vitro effect of statins on process outgrowth in OLN-93 cells, a well-characterized OLG-derived cell line, and primary cultures of neonatal rat OLGs.

Analysis of mitochondrial pH and ion concentrations.

Detailed practical information is provided with emphasis on mapping cytosolic and mitochondrial pH, mitochondrial Na(+), and briefly also aspects related to mitochondrial Ca(2+) measurements in living cells, as grown on (un)coated glass coverslips. This chapter lists (laser scanning confocal) microscope instrumentation and setup requirements for proper imaging conditions, cell holders, and an easy-to-use incubator stage. For the daily routine of preparing buffer and calibration solutions, extensive annotated protocols are provided.

Morphological changes do not reflect biochemical and functional differentiation in OLN-93 oligodendroglial cells.

OLN-93 cells, a cell line established from spontaneously transformed rat brain glial cultures, are used as a model for oligodendrocytes. These cells are known to undergo morphological changes upon serum deprivation. The objective of the present study is to investigate a possible correlation between these morphological changes and (1) the loss or gain of oligodendrocyte markers and (2) the electrophysiological properties of these cells.

Assessment of activated sludge stability in lab-scale experiments.

The majority of activated sludge research is conducted in a laboratory environment with, most often, the start-up sludge being taken from a large-scale wastewater treatment plant. Inoculating this sludge in a lab-scale set-up induces a transient period, which, evidently, has a direct impact on the experimental results during this period of acclimatization. In the currently published literature, the acclimatization period is either neglected or fixed to two or three times the sludge age, without any guarantee that stable conditions are indeed reached.

Characterisation and optimisation of individual wastewater treatment systems.

Onsite individual wastewater treatment systems can provide a financially attractive alternative to a sewer connection in locations far from the existing sewer network. These systems are, however, relatively new, and practical experiences, especially long-term field studies, are lacking. Therefore, a thorough study of two compact biofilm-based, aerobic onsite systems, both of five population equivalents, was started in 2001.

Efficient tracking of the dominant eigenspace of a normalized kernel matrix.

Various machine learning problems rely on kernel-based methods. The power of these methods resides in the ability to solve highly nonlinear problems by reformulating them in a linear context. The dominant eigenspace of a (normalized) kernel matrix is often required.

Optimal fed batch experiment design for estimation of monod kinetics of Azospirillum brasilense: from theory to practice.

In this paper the problem of reliable and accurate parameter estimation for unstructured models is considered. It is illustrated how a theoretically optimal design can be successfully translated into a practically feasible, robust, and informative experiment. The well-known parameter estimation problem of Monod kinetic parameters is used as a vehicle to illustrate our approach.

Detection of filamentous bulking problems: developing an image analysis system for sludge composition monitoring.

This article describes a fully automatic image analysis procedure for fast and reliable characterization of the activated sludge composition, that is, the floc and filament features. The algorithms developed for each of the analysis steps, that is, segmentation, object recognition, and characterization, are described in detail. Although the application range of the recognition method is a priori expanded by introducing a number of control parameters, the procedure proves to be intrinsically robust as it produces satisfactory results for a fixed set of parameter values for a wide variety of image types.

Role of mitochondrial Na+ concentration, measured by CoroNa red, in the protection of metabolically inhibited MDCK cells.

In ischemic or hypoxic tissues, elevated cytosolic calcium levels can induce lethal processes. Mitochondria, besides the endoplasmic reticulum, play a key role in clearing excessive cytosolic Ca2+. In a previous study, it was suggested that the clearance of cytosolic Ca2+, after approximately 18 min of metabolic inhibition (MI) in renal epithelial cells, occurs via the reverse action of the mitochondrial Na+/Ca2+ exchanger (NCX).

Ca2+ uptake in mitochondria occurs via the reverse action of the Na+/Ca2+ exchanger in metabolically inhibited MDCK cells.

In ischemic or hypoxic tissues, elevated Ca2+ levels have emerged as one of the main damaging agents among other Ca2+-independent mechanisms of cellular injury. Because mitochondria, besides the endoplasmic reticulum, play a key role in the maintainance of cellular Ca2+ homeostasis, alterations in the mitochondrial Ca2+ content ([Ca2+]m) were monitored in addition to changes in cytosolic Ca2+ concentration ([Ca2+]i) during metabolic inhibition (MI) in renal epithelial Madin-Darby canine kidney (MDCK) cells. [Ca2+]i and [Ca2+]m were monitored via, respectively, fura 2 and rhod 2 measurements.