[The comparative evalution of informativeness of immunologic and molecular genetic methods and means during stages of specific indication of melioidosis agent].

The reference-center of monitoring of agents of glanders and melioidosis carried out testing of reagents kits for diagnostic of agent of melioidosis and other close-related species of Burkholderiae in vitro. At the stage of specific identification of pathogenic Burkholderiae the diagnostic possibilities of commercial and experimental kits of reagents for express- and rapid analysis were evaluated. The criteria of evaluation of diagnostic value of kits of reagents were sensitivity, specificity and time of implementation of studies.

[Comparative characteristic of Burkholderiae pseudomallei group].

Aim: Comparative characteristic of diagnostic value of main cultural-biological characteristics of Burkholderiae pseudomallei group.

Materials And Methods: 59 strains of B. pseudomallei, 14 --B.

[Study of extracellular antigens by immunodiffusion methods in differentiation of pathogenic burkholderiae].

Aim: Isolation and composition comparison of extracellular antigens (ECA) of pathogenic burkholderiae in SDS-PAGE electrophoresis and their use for differentiation of these microorganisms by immunodiffusion methods.

Materials And Methods: 60 Burkholderia pseudomallei strains, 14 B. mallei strains, 5 B.

[Experimental study on chemotherapy of acute glanders].

Glanders is a zoonotic infection inducing acute forms of the disease (pneumonia, sepsis) in humans and animals under certain conditions, which even with the use of modern chemotherapy have unfavourable prognosis. Insufficient of efficacy of antibiotics with in vitro low MIC for planktonic bacterial suspension of Burkholderia mallei in chemotherapy of acute forms of glanders was due to the capacity of the pathogen for intracellular survival and formation of biofilms. Under such conditions the susceptibility of B.

[Bacteriocins and bacteriophages of pathogenic Burkholderia].

Aim: Detection of bacteriocins and phages in pathogenic bacteria of Burkholderia genus and study of their specificity range.

Materials And Methods: Sixty strains of B. pseudomallei, 11 strains of B.

[In vitro antibiotic susceptibility compliance with efficacy of chemotherapy in infections due to pathogenic Burkholderias].

Among the known species of Burkholderia only two are obligate pathogens, i.e., B.

[Phenotypic and genotypic identification of bacteria from the Burkholderia cepacia complex].

Aim: Evaluation of the diagnostic value of pheno- and genotypic characteristics of B. cepacia strains collection.

Materials And Methods: Phenotypic and genetic methods of identification and differentiation of 25 strains of the B.

[Procedure for preliminary rapid estimation of bacteria susceptibility to chemotherapeutics].

Principles and procedure for rapid estimation of bacteria susceptibility to antibiotics on a glucose-tryptone medium with an indicator are described. The results of the tests with 50 microbial strains of 17 species showed practically complete identity to the results of the antibiograms when the data were estimated in 3-6 hours according to the described procedure in comparison to the findings estimated in 18-24 hours on the standard media.

[The use of polymerase chain reaction for detection of the agents of glanders and melioidosis using experimental infection].

Glanders and melioidosis are severe infectious diseases of people and animals. The causative agents of these infections refer to the potential agents of bioterrorism of group B. In this work the possibility of use of flagellin-based primers for the identification of B.

[The use of multilocus sequence typing (MLST) and randomly amplified polymorphic DNA (RAPD) for the differentiation between strains of Burkholderia mallei].

Burkholderia mallei is highly pathogenic microorganism for both humans and animals. In this work, the possibility of the use of the genotyping method for differentiation between strains of B. mallei was studied.

[Adequate selection of antigens for serologic diagnostics of experimental melioidosis].

Sera of Burkholderia pseudomallei-infected golden hamsters, white mice, guinea pigs and white rats were studied. Immunochemical analysis revealed presence of antibodies against antigens 2, 3, 6, d, and g with significant predominance of antibodies to antigens 6 and d. Antigen d was detected irrespectively to strain used for experimental infection and experimental animal species.

[Comparative analysis of total cell protein electrophoregram of pathogenic Burkholderia].

Whole-cell proteins of 22 strain of Burkhoderia pseudomallei, including 13 B. mallei, 5 B. cepacia strains and 14 strains of opportunistically pathogenic Pseudomonas defined by 1D SDC-PAAG electrophoresis.

[Molecular-genetic approaches to diagnosis and intraspecific typing of causative agents of glanders and melioidosis].

Pathogenic Burkholderia--Burkholderia mallei and Burkholderia pseudomallei--are causative agents of glanders and melioidosis, severe infectious diseases of man and animals. They are regarded as potential agents of bioterrorism. The existing bacteriological and immunological methods of identification of B.

[Cross-reacting antigens of pathogenic Burkholderia and some dangerous causative agents of infectious diseases].

Cross-reacting antigens in B. mallei, B. pseudomallei, B.

[Experimental study on the possibility of using live tularemia vaccine to increase resistance to heterologous infection disease].

In experiments on guinea pigs immunized with Francisella tularensis 15, or live tularemia vaccine (LTV), the level of heterologous protective effect to dangerous infectious diseases caused by Yersinia pestis, Burkholderia pseudomallei, B. mallei, Mycobacterium tuberculosis was studied. The study revealed that during the first 4 weeks after the subcutaneous immunization with LTV the level of resistance of the immunized animals to heterologous infective agent reliably increased as indicated by the survival rate of the animals, as well as by the survival time of those killed by infection, in comparison with the controls.

[Use of PCR for identification of Burkholderia mallei].

Stimuli of glanders belong to the potential agents of biological terror. The possibility to use various primers in the identification of B. mallei was investigated and the significance of polymerase chain reaction (PCR) was defined within the scheme of laboratory glanders diagnosis in the offered paper.

[Identification of the causative agents of glanders and melioidosis by polymerase chain reaction].

Burkholderia mallei and B. pseudomallei are causative agents of glanders and melioidosis, respectively, i.e.

[Effect of cultivation temperature on the extrachromosomal hereditary factors of the causative agent of melioidosis].

The cultivation temperature of Burkholderia pseudomallei has been shown to determine both the direction of morphological dissociation and the prophage induction rate. Inheriting plasmid replicons was found to depend on the temperature conditions during the growth of these bacteria. No influence of B.

[Burkholderia thailandensis: biological properties, identification and taxonomy].

Burkholderia pseudomallei-like microorganisms have been isolated from soil and water in regions with endemic melioidosis. These strains have biochemical and antigenic profiles identical to melioidosis agents, except that they differ by virulence and L-arabinose (vir-, ara+). There are minor differences between these species by rRNA sequence.

[Pathogenicity of Burkholderia pseudomallei: extracellular and surface antigen functions].

The data of literature and the results of investigations carried out by the authors on the analysis of B. pseudomallei pathogenicity factors. They include mucoid, endotoxin, lecithinase, proteases, hemolysins, etc.

[The outlook for the development of live vaccines for the prevention of melioidosis].

The effectiveness of immunization with Burkholderia pseudomallei attenuated strains (Pur and Ts), heterologous vaccines and the recombinant culture of Francisella tularensis RM2 carrying a plasmid with fragments of B. pseudomallei chromosome was studied in four species of experimental animals, essentially differing in their sensitivity to melioidosis. The most immunogenic B.

[The efficacy and outlook for the study of live vaccines for the prevention of melioidosis].

The effect of immunization with Burkholderia pseudomallei, (Pur- and Ts), heterologous vaccines and the recombinant culture of Francisella tularensis RM2, carrying a plasmid with fragments of B. pseudomallei chromosome, was studied on four species of experimental animals, essentially differing by their sensitivity to melioidosis. B.

[Isolation and primary characteristics of a Pseudomonas (Burkholderia) pseudomallei plasmid].

Plasmid screening of reference Pseudomonas pseudomallei strains isolated from patients and animals revealed cryptic plasmids with different molecular weights in 30 strains. Plasmids were investigated by restriction analysis and DNA-DNA hybridization. Cryptic plasmids were denoted as pPM1, pCM2, pCM3, and pCM4.

[Effect of medium temperature and pH on the sensitivity of pathogenic pseudomonads to chemotherapeutic agents].

Antibiotic susceptibility of four Pseudomonas species i.e. P.