Changes in Expression of Key Genes in Ceca of Chicken Broilers as Affected by Glyphosate, Antibiotics and a Coccidiostat.

Studies have shown the presence of residual amounts of the herbicide glyphosate in poultry feed, which leads to its bioaccumulation in the body. Recently, it has been established that exposure to low levels of glyphosate over a long period may have serious negative effects on poultry health. Moreover, combined exposure to several toxicants can potentially lead to additive and/or synergistic effects.

Assessing the Risk of Spreading and Its Toxins Within the Dairy Farm.

currently constitutes a major pathogen of the gastrointestinal tract, which poses a significant growing burden on medicine and veterinary medicine in many regions. A farm was assessed (feed table, silage pit, and feces (healthy animals, emaciated animals, and animals with mastitis)) for the presence of toxins using the PCR method and for the microbiome in cow feed and feces using NGS technology, one month apart. toxin A and binary toxin were detected in feed samples.

An Investigation on the Effects of Partial Replacement of Rapeseed Cake in Ayrshire Dairy Heifers' Diets with By-Product Source of Animal Protein on Body Weight Dynamics, Nutrient Balancing, and Blood Biochemical Markers.

Recently, the secondary use of by-products of the processing industry resulting from the production of poultry meat, which can be included in animal diets, has become a popular topic in the feed industry. For studying the effects of partial replacement of rapeseed cake (RC) with the by-product source of animal protein concentrate Agro-Matic (PCAM) on growth dynamics, nutrient absorption and nitrogen balance, as well as blood biochemical parameters during the growing period, a total of 48 Russian Ayrshire dairy heifers were selected for this experiment and they were divided into three experimental groups (16 in each group, including the control group). The heifers of the control group were fed the basal diet which contained rapeseed cake (30%), while the second (RC + PCAM) and third groups (PCAM + RC) were fed the basal diet after replacing a part of the rapeseed cake with 2.

Probiotics as an alternative to antibiotics in modulating the intestinal microbiota and performance of broiler chickens.

Aims: Gut bacteria play an important role in poultry nutrition and the immune defense system. Changes in the intestinal microbiome affect the physiological state, metabolism, and innate immunity of poultry. The present study aimed to characterize age-related changes in the gastrointestinal tract microflora in broiler chickens, depending on supplementation of the diet with the in-feed antibiotic Stafac® 110 and a Bacillus subtilis strain-based probiotic.

Investigating adverse effects of chronic dietary exposure to herbicide glyphosate on zootechnical characteristics and clinical, biochemical and immunological blood parameters in broiler chickens.

Although the herbicide glyphosate is widely used globally and considered safe, more evidence of its adverse effects on animals and humans is accumulating. The present investigation was aimed at evaluating the impact of different glyphosate concentrations on zootechnical characteristics and clinical, biochemical and immunological blood parameters in Ross 308 broiler chickens. Four groups were employed, including untreated control and three experimental groups fed diets enriched with glyphosate at doses of 10, 20 and 100 ppm that conformed to 0.

Hyperlipoproteinemia(a) and Severe Coronary Artery Lesion Types.

Diffuse atherosclerosis and calcification of the coronary arteries (CA) create serious difficulties for coronary artery bypass grafting (CABG). The aim of this study was to compare demographic indicators, lipids, and clinical results one year after CABG in patients with different phenotypes of coronary artery (CA) disease. In total, 390 patients hospitalized for elective CABG were included in a single-center prospective study.

Influence of Using Various Levels of Protein Concentrate in Rations of Ayrshire Dairy Cows on Rumen Microbiome, Reproductive Traits and Economic Efficiency.

Animal feeding research has revealed a close relationship between the chemical composition and nutritional value of cow rations, the number of rumen bacterial communities and animal productivity. Our present research aimed to investigate the outcome of inclusion of different levels of protein concentrate in rations of Ayrshire dairy cows in relation to the rumen microbiome, reproductive traits and economic value. Forty-five Ayrshire cows were divided into three groups (15 in each).

The structure and functional profile of ruminal microbiota in young and adult reindeers () consuming natural winter-spring and summer-autumn seasonal diets.

Background: The key natural area of Russian reindeer (, Nenets breed) is arctic zones, with severe climatic conditions and scarce feed resources, especially in the cold winter season. The adaptation of reindeer to these conditions is associated not only with the genetic potential of the animal itself. The rumen microbiome provides significant assistance in adapting animals to difficult conditions by participating in the fiber digestion.

Intra-soil waste recycling provides safety of environment.

Amelioration and remediation technology was developed for phosphogypsum utilization in Haplic Chernozem of South-European facies (Rostov Region). The technology comprises phosphogypsum dispersed application into the soil layer of 20-45 cm during intra-soil milling. In the model experiment, the phosphogypsum doses 0 (control), 10, 20, and 40 t ha were studied.

The Comparative Analysis of the Ruminal Bacterial Population in Reindeer ( L.) from the Russian Arctic Zone: Regional and Seasonal Effects.

The reindeer ( L.) is a unique animal inhabitant of arctic regions. Low ambient temperatures and scant diets (primarily, lichens) have resulted in different evolutional adaptations, including the composition of the ruminal microbiota.

Effects of Essential Oils-Based Supplement and Infection on Gene Expression, Blood Parameters, Cecal Microbiome, and Egg Production in Laying Hens.

One of the main roles in poultry resistance to infections caused by is attributed to host immunity and intestinal microbiota. We conducted an experiment that involved challenging Lohmann White laying hens with Enteritidis (SE), feeding them a diet supplemented with an EOs-based phytobiotic Intebio. At 1 and 7 days post-inoculation, the expression profiles of eight genes related to immunity, transport of nutrients in the intestine, and metabolism were examined.

Examination of the Expression of Immunity Genes and Bacterial Profiles in the Caecum of Growing Chickens Infected with Enteritidis and Fed a Phytobiotic.

This study was performed to investigate the differential expression of eight immunity genes and the bacterial profiles in the caecum of growing chickens challenged with serovar Enteritidis (SE) at 1 and 23 days post inoculation (dpi) in response to SE infection at 19 days of age and administration of the phytobiotic Intebio. Following infection, the genes and were upregulated by greater than twofold. Chicks fed Intebio showed at 1 dpi upregulation of , , , and .

Association of lipoprotein(a) level with short- and long-term outcomes after CABG: The role of lipoprotein apheresis.

Objective: To evaluate the association of lipoprotein(a) [Lp(a)] level with short- and long-term outcomes after coronary artery bypass grafting (CABG) and to assess the effect of a 12 month course of weekly lipoprotein apheresis on vein graft patency and coronary atherosclerosis course in post-CABG patients with hyperlipidemia.

Methods: This study was performed in patients after successful CABG and consisted of three parts: a) a retrospective part with computed tomography assessment of vein graft patency in patients with first-year recurrence of chest pain after CABG (n = 102); b) a prospective trial with evaluation of cardiovascular outcomes during follow up time up to 15 years in relation to baseline Lp(a) levels (n = 356); c) an 12-months interventional controlled study in 50 patients with low-density lipoprotein cholesterol (LDL-C) levels >2.6 mmol/L prior to the operation despite statin treatment that allocated into 2 groups: active (n = 25, weekly apheresis by cascade plasma filtration (CPF) plus atorvastatin), and control (n = 25, atorvastatin alone).

Broiler Cecal Microbiocenoses Depending on Mixed Fodder.

Molecular genetic techniques (NGS sequencing and quantitative PCR) were used to determine the composition of the cecal bacterial community of broiler chickens fed with different mixed fodder. The Cecal microbiome exhibited taxonomic diversity, with both typical inhabitants of avian intestine belonging to the families Clostridiaceae, Eubacteriaceae, and Lactobacillaceae and to the phylum Bacteroidetes, and new un- identified taxa, as well as bacteria of the families Lachnospiraceae and Ruminococcaceae, which were previ- ously considered restricted to the rumen microflora. Contrary to traditional concepts, enterococci and bi- fidobacteria were among the minor components of the community, lactate-fermenting species were absent, and typical avian pathogens of the genus Staphylococcus were detected but seldom.

Terminology spectrum analysis of natural-language chemical documents: term-like phrases retrieval routine.

Background: This study seeks to develop, test and assess a methodology for automatic extraction of a complete set of 'term-like phrases' and to create a terminology spectrum from a collection of natural language PDF documents in the field of chemistry. The definition of 'term-like phrases' is one or more consecutive words and/or alphanumeric string combinations with unchanged spelling which convey specific scientific meanings. A terminology spectrum for a natural language document is an indexed list of tagged entities including: recognized general scientific concepts, terms linked to existing thesauri, names of chemical substances/reactions and term-like phrases.

Metagenomic bacterial community profiles of chicken embryo gastrointestinal tract by using T-RFLP analysis.

Thirty microbial phylotypes of microorganisms were found in the gastrointestinal tract of chicken belonging to the Hajseks White breed, and 38 phylotypes were found in the gastrointestinal tract of chicken belonging to the Hajseks Brown breed. The microbiome of the gastrointestinal tract of the chicken embryos of the Hajseks White breed was dominated by the typical representatives of avian intestinal microflora--bacteria of the family Enterobacteriaceae (47.3%), orders Actinomycetales (13.

Cascade plasma filtration during the first year after CABG in patients with hyperlipidemia refractory to statins.

Objective: To evaluate the effect of a 12-month course of weekly lipid apheresis on vein graft patency after coronary artery bypass grafting (CABG) in patients with hyperlipidemia refractory to statins.

Methods: In a 12-month prospective controlled clinical trial we enrolled 34 male patients (mean age 57 ± 8 years) who passed through successful CABG and low-density lipoprotein cholesterol (LDL-C) level >2.6 mmol/L prior to the operation despite statin treatment.

[High level of lipoprotein (a) as a predictor of poor long-term prognosis after coronary artery bypass surgery].

Study aim was to investigate the association of lipoprotein (a) [Lp(a)] level with the development of cardiovascular complications in long-term follow-up period after coronary artery bypass grafting (CABG). Patients with chronic ischemic heart disease (IHD) (n = 361, 88% men, mean age 55 +/- 9 years) who had had CABG were included in the study. Before surgery we assessed presence of classical risk factors, left ventricular ejection fraction, concentrations of lipids and Lp(a) in blood serum.

[Evolutionary transformations of the malacofaunas in the Neogene basins of paratethys as an example of development of the ecosystems of insular type].

Evolution of the bivalve and gastropod mollusks is considered under conditions of the biochore isolation, ecosystem impoverishment, and competition weakened in the Cenozoic marine half-closed and nearly closed brackish basins during the last 30 million years (Oligocene to Pliocene). In these environments, the mollusks have first undergone drastic impoverishment of the taxonomic composition at expense of stenobionts, followed by a rapid diversification of few survived eurybiotic taxa accompanied by formation of numerous endemic species, genera, and occasionally families. Diversification rates used to rise drastically with the stability disturbance in the benthic ecosystems.

[Ligand-induced structuring of polyreactive immunoglobulins].

The influence of several hydrophobic compounds with different structures on the binding of native and chaotropically modified bovine immunoglobulins to native and denatured nonspecific protein immobilized on the surface is researched. It is shown that the character of binding depends on the amount and mutual location of hydrophobic nuclei in the effectors structure. The authors observed moderate suppression of binding or absence of the expressed effect while using one-nucleus effector.

Association of lipoprotein(a) excess with early vein graft occlusions in middle-aged men undergoing coronary artery bypass surgery.

Objective: To assess the relationship of lipoprotein(a) to early vein graft occlusions in patients after coronary artery bypass grafting.

Methods: We studied 102 male patients (mean age 52.3 +/- 8.