[The neuroradiological aspects of infantile spasms].

The observation of 38 children with early form of children epilepsy that is with syndrome of infantile spasms (IS) was performed by means of computed tomography of the brain. The structural alterations of the brain were revealed in 89.5% of cases.

[The pathological mechanisms of the intermenstrual pain syndrome].

Comprehensive clinicophysiological examinations of 80 patients suffering from the intermenstrual pain syndrome revealed organic changes in the pelvic organs of 92.5% of women and disturbances in the central structures participating in the conduction and control of pain sensitivity and vasomotor regulation in all the examinees, these changes correlating with specific vegetovascular and psychoemotional disorders. Three pathogenetic variants of development of this syndrome were defined.

[West syndrome in tuberous sclerosis].

Observation's analysis of 5 children in age from 9 months to 4 years 11 months with tuberous sclerosis was performed. The initial manifestations of disease were following: early children form of epilepsy exactly infantile spasms (West syndrome) appearance, dermal alterations in the form of depigmented spots as well as nonprogressive delay in psychoverbal development. Together with clinical symptoms the main criterion in early form of tuberous sclerosis diagnosis determination turned out to be brain's specific alterations (tubers) which were revealed by computer tomography.

[Possibilities of the use of gas-liquid chromatography in the identification of Nocardia microorganisms].

By determination of fatty acid composition of bacterial cells, gas-liquid chromatography can quickly identify Nocardia microorganisms isolated from the infected material. The study of fatty acid spectrum of the reference and fresh cultures Nocardia has found their homogeneity. Such fatty acids as hexadecenoic, hexadecanoic, octadecenoic, octadecanoic and tuberculostearic acids occurred most frequently.

[Study of the structural organization of OSCP--subunits of H+-ATPase of porcine heart mitochondria].

Unmodified and citraconilated OSCP of the pig heart mitochondrial H(+)-ATPase were hydrolysed by proteinase from Staphylococcus aureus V8 and trypsin, respectively. To purify the individual peptides, various types of HPLC and covalent chromatography on SH-Sepharose were used. By the automatic Edman method complete or partial amino acid sequences of the peptides obtained were determined, thus allowing for the reconstruction of the primary structure of pig OSCP.

[Cloning, determination of primary structure, and expression of the C-terminal segment of human cholesterol-esterase/lipase, containing the antigenic determinant of the protein, in Escherichia coli].

Clone pHICE0.9 was selected from human insulinoma cDNA library by immunoscreening with antibodies against total human insulinoma proteins. This clone contains a 0.

[Autonomic nervous system activity during the normal menstrual cycle].

Body adaptation reactions to changes of the internal conditions (neurohumoral shifts related to ovulation and corpus luteum development) were studied in 15 women of a reproductive age with normal biphasic cycle in the early follicular and medium lutein phases on the basis of mathematical analysis of heart rhythm, a number of cardiovascular and respiratory system parameters, and brain electric activity. Moderate tension of the regulatory mechanisms in the lutein phase of the cycle was protective and adaptive and probably related to body preparation to a would-be pregnancy. These adaptive effects are different in different women: in some internal environment parameters vary within a wide spectrum, in others these parameters show a trend to retain their functioning regimen.

[Study on structural gene expression in human insulinoma].

A human insulinoma cDNA library was constructed in the expression plasmid vector pUEX1. The clone pUEX1Ins12 was selected by means of hybridization with an insulin probe. It codes for full size amino acid sequence preproinsulin.

[The pathogenetic mechanisms of hyperthermia in patients with the premenstrual syndrome].

Functional status of the central and autonomic nervous system was analyzed in 28 patients with the premenstrual syndrome one of whose manifestations was disordered thermoregulation. The examinations were carried out during both cycle phases. Analysis of the EEG data and clinical findings has shown different patterns of autonomic thermoregulation disturbances in three groups of patients and permitted the authors to suggest that changed activity of certain neurotransmitter systems connected with hormonal secretion over the course of the cycle was responsible for these disturbances.

[Clinical and genetic analysis of the Townes-Brocks syndrome].

Due to the performed analysis of data from literature and the author's observations of the Taunse-Brock's syndrome a wide variability of phenotypic manifestations is shown, the diagnostic criteria of syndrome are determined, its autosomic-dominant inheritance with complete penetrance is confirmed.

[Clinical-genealogic analysis of diaphragmatic hernias].

The results of clinical-genetic examination of 174 probands with congenital diaphragmatic hernias and their families are presented. Genetic heterogeneity of diaphragmatic hernias, the spectrum of inherited syndromes obtained in the present study and shown in literature the spectrum and frequency of congenital malformations accompanying diaphragmatic hernias were shown. No increase in the average age of the probands' parents and in the marriage distances changes was observed both for isolated diaphragmatic hernias and those accompanied by other malformations was observed.

[Cloning, primary structure determination and expression of preproinsulin cDNA from human insulinoma in Escherichia coli].

A human insulinoma cDNA library was constructed in expression plasmid vector pUEX1. Clone pUEX1Ins12 was selected from human insulinoma cDNA library by means of hybridization with the insulin probe and a nucleotide sequence of the insertion was determined. It codes for full size amino acid sequence preproinsulin and furthermore, contains the entire 3'-end of noncoding mRNA region and 44 nucleotides from the 5'-untranslated region.

[10-year experience with correction of supraventricular tachycardia].

The results of a 10-year experience in surgical treatment of supraventricular tachyarrhythmias in 258 patients are analysed. Invasive and noninvasive methods of electrophysiological studies with the predominant use of Soviet-produced apparatuses were employed in establishing the diagnosis. Surgery of arrhythmias included catheter destruction, cryosurgery on a working heart, but the prevailing methods were various types of destruction of anomalous conducting paths under conditions of extracorporeal circulation.

[Cerebrovascular circulation in patients with oligomenorrhea].

Brain hemodynamics was studied in 76 patients with oligomenorrhea aged 18 to 35 from the data of rheoencephalography. Disorders of the regional tone of brain vessels were different and related to the time of the menstrual cycle disorder; this may be explained by the degree of changes in various neuromediator mechanisms of brain circulation and reproductive system function regulation.

[General principles of the pathogenesis of vegetovascular and psychoemotional disorders during menstrual cycle and methodological approaches to their study].

Analysis of charts filled in by 79 women (mean age 31.5 +/- 4.0 years) over the course of 2-3 menstrual cycles has demonstrated the regular manifestation of various psychosomatovegetative symptoms over the entire course of the cycle (in the follicular, lutein phases, and during ovulation).

[Characteristics of the functional state of different sections of the autonomic nervous system and their role in the pathogenesis of oligomenorrhea].

Analysis of the clinical picture of oligomenorrhea and of the results of studies of the function of various sections of the autonomic nervous system (ANS) in 58 patients of a reproductive age has revealed differences in these functions, related to the time of menstrual disorders onset. Oligomenorrhea patients with menarche complained of cardiovascular, respiratory, and emotional-motivational disorders, examinations have revealed in them dysfunctional disorders and reduced reserve potentials of both ANS sections. In oligomenorrhea patients in whom the condition developed at the age of 18-25 after a period of regular menstruations the effects of various stress factors resulted in reduction of the functional activity of the ergotropic (sympathoadrenal) section of the ANS.

[The establishment of the presence of feces in stains by agar gel electrophoresis].

An electrophoretic method of feces determination in stains was developed. Method is based on detection of acid phosphatase only in feces since it is not detectable in human blood and other secretions by this method.

[Characteristics of the functional state of the central nervous system in women during the menstrual cycle and in premenstrual syndrome].

Electroencephalographic examination of the function of the central nervous system in 30 healthy women and in 15 ones suffering from the premenstrual syndrome, carried out over both phases of the menstrual cycle, has revealed clear-cut differences between the two groups. 46% of the patients developed pathologic shifts at the level of the brain stem, 37% of the hypothalamic level, and 17% combined involvement of these portions of the brain. Triggering of the compensatory defense mechanisms already during the follicular phase of the cycle indicates failure of the adaptation mechanisms in the patients with the premenstrual syndrome.

[The establishment of the presence of sperm in stains by an agar gel electrophoretic method].

Comparative investigation of seminal stains and other secretions from human body and blood as well as objects of plant origin by gel electrophoresis using alkaline values of pH buffer solutions and subsequent enzymography for acid phosphatase made it possible to develop optimal conditions for detection of seminal presence in stains. Good preservation of seminal acid phosphatase in stains during several years is shown.

[A new case of a syndrome including marfanoid phenotype and craniostenosis].

A new case of the syndrome with craniosynostosis and Marfanoid features is reported. The data presented and the analysis of relevant literature are suggestive of a community of the Marfanoid features with clinical and genetic heterogeneity. The possibility to delineate the Marfanoid syndrome with craniosynostosis as a nosologic unit and its etiology are discussed.