ANALYSIS OF POPULATION IMMUNITYAGAINST INFLUENZA PRIOR TO 2014 AND 2015 EPIDEMIC SEASONS.

Aim: Control for the population herd immunity against seasonal influenza viruses as well as for emergence of antibodies against influenza with pandemic potential in human blood sera.

Materials And Methods: HAI reaction against vaccine and epidemic influenza viruses as well as HPAI viruses A/rook/Chany/32/2015 (H5N1) (clade 2.3.

[Analysis of population immunity of residents of Russian Federation regions against seasonal and highly pathogenic strains of influenza virus].

Aim: Determine the level of antibodies against socially significant types/serotypes of influenza virus in sera of individuals residing in various regions of Russia.

Materials And Methods: 1525 samples of blood sera collected in August-December 2013 in 8 regions of Russian Federation were studied in hemagglutination inhibition reaction (HAI) with antigens obtained from A/California/07/09, (H1N1)pdm09, A/Victoria/361/2011(H3N2), B/Brisbane/60/2008 (Victoria line), B/Massachusetts/2/2012 (Yamagata line), A/Commongull/Chany/2006 (H5N1), A/Anhui/01/2013 (H7N9) influenza virus strains.

Results: None of the blood sera samples had significant HAI titers against A/H5 and A/H7 antigens.

[Monitoring for influenza in population of Western Siberia in 2007-2009].

Aim: To analyze influenza viruses isolated in the 2008-2009 autumn-winter season, and to test sera collected in the south of Western Siberia during the beginning and the end of the epidemic seasons from 2007 until the A/H1N1 pandemic.

Materials And Methods: Total 149 clinical samples were analyzed and 2190 blood sera were tested. During the 2008-2009 season 17 influenza viruses were isolated.

[Detection of antibodies to A/H5N1 influenza virus in citizens of Russian Federation].

Aim: To determine levels of antibodies to influenza virus A/H5N1 in serum samples of people living in different regions of Russia in order to assess the risk of infection with avian influenza H5N1.

Materials And Methods: Two thousand one hundred sixty-eight serum samples were tested by hemagglutination inhibition assay for the presence of antibodies to influenza virus A/H5N1.

Results: Twenty-six serum samples obtained from residents of Khanty-Mansiysk Autonomous Area and 2 samples from residents of Novosibirsk region were positive for antibodies to serotype A/H5.

[Hemagglutination inhibition test for retrospective diagnosis of avian influenza in mammals].

Aim: To compare usage of native and formalinized erythrocytes from different animal species in hemagglutination inhibition (HI) test for detection of level of specific antibodies to H5N1 influenza virus in sera of mammals.

Materials And Methods: Level of anti-H5 antibodies to influenza H5 control antigen and to influenza viruses A/Common gull/Chany/2006 (H5N1), A/duck/Tuva/01/06 (H5N1), A/Anas platyrhynchos/Chany Lake/9/03 (H5N3) was determined by hemagglutination inhibition test in two influenza A (H5) reference antisera as well as in ferret antisera to native strains of avian influenza virus. Equine, rhesus macaque, sheep, guinea pig, goose, and chicken erythrocytes were used.

[Monitoring of antibodies to influenza A virus in populations of different regions of West Siberia].

Aim: To study levels of antibodies to influenza virus in sera of subjects residing in different regions of West Siberia in order to assess the risk of infection with avian influenza virus H5N1.

Materials And Methods: Two hundred and sixty-five serum samples were tested for the presence of antibodies to influenza virus A/New Caledonia/99 (H1N1), A/New York/55/2005 (H3N2), A/Whooper swan/ Mongolia/244/2005 (H5N1) by hemagglutination inhibition assay (HAI) and reaction of microneutralization.

Results: All tested sera were negative for antibodies to H5N1.

[Antiviral activity of vegetable triterpens, their derivatives and ribavirin phosphonates].

The purpose of the study was to investigate, in the Vero cell culture, the antiviral activity of vegetable tritrpens derivatives and ribavirin analogues against the viruses of measles, herpes simple (type 1), cytomegaloviruses and filoviruses. The toxicity and antiviral activity of compounds were determined after coloring of cells with crystal violate. Additionally, the combined action of triterpens' derivatives and ribavirin was investigated.

[Immunoenzyme test-system for detection of glycyrrhizic acid].

An experimental enzyme immunoassay test kit was developed for detecting glycyrrhizic acid (GA) during pharmacokinetic investigations. This method was used to determine the GA content in the blood plasma of mice and guinea pigs after intraperitoneal and intravenous injections. The results of the GA determination using the new test kit agree with the data obtained by HPLC.

[Immunostimulating effect of plant triterpenes and their derivatives].

The immunostimulating activity of triterpenes of vegetable origin and their derivatives was studied. The study revealed that glycyrrhizic and betulonic acids, as well as derivatives of ursolic and glycyrrhetinic acids, enhanced the level of specific antibodies to bovine serum albumin in mice.

[Content of progesterone, glucocorticoid and glycyrrhizic acid receptors in normal and tumoral human breast tissue].

Progesterone and glucocorticoid receptor levels were assayed in breast tumor. The progesterone levels in tumor were 1.2-2.

[Preparation of a specific immunogen based on bacteriophage M13].

Earlier we developed an expression vector on the basis of bacteriophage M13 allowing the exposure of short peptides on the virion surface. It was used to obtain a recombinant phage carrying the antigenic determinant of HIVI gag protein p17. This phage was tested as immunogen in rabbits.

Activity of cat parietofrontal neurons during the performance of a voluntary movement.

The impulse activity of 227 neurons of field 5 was investigated in cats trained to complete a movement of the forepaw in response to a stimulus. The discharges of each of them were collected relative to three markers: the presentation of the conditional signal, the raising of the paw from a support, and pressing on a pedal. The reactions associated with the performance of the reflex were recorded in 224 neurons.

[The activity of the parietofrontal neurons in the cat performing voluntary movement].

Unit activity was recorded in area 5 of the alert cat parietal cortex during trained movement of a contralateral forepaw. 224 of 227 cells modified their activity by producing triggering stimulus and/or realizing the movement. The movement-related activity of 67.