The study focuses on finding a strong inducible promoter for expressing proteins in the green microalga Chlamydomonas reinhardtii, specifically leveraging the CrGPDH3 gene, which is activated by NaCl treatment.
Researchers identified and characterized a 3012 bp promoter region (PromA) and determined a core promoter (PromC) that allows basal expression through deletion analysis with GUSPlus as a reporter gene.
Findings suggest that certain regions within this promoter can respond to salt, making them effective for driving transgene expression, thus advancing the potential for using CrGPDH3 and its derivatives in genetic engineering applications in C. reinhardtii.