Publications by authors named "Ildefonso Silva"
ELTD1/ADGRL4 expression is increased in the vasculature of a number of tumor types and this correlates with a good prognosis. Expression has also been reported in some tumor cells with high expression correlating with a good prognosis in hepatocellular carcinoma (HCC) and a poor prognosis in glioblastoma. Here we show that 35% of primary human breast tumors stain positively for ELTD1, with 9% having high expression that correlates with improved relapse-free survival.
View Article and Find Full Text PDFAlterations in monocyte subsets and cytokine production after TLR activation in American Cutaneous Leishmaniasis.
Parasite Immunol
May 2019
Phenotypic and functional aspects of monocytes from Localized Cutaneous Leishmaniasis (LCL) patients were evaluated. The frequencies of monocyte subsets and TLR2/TLR4 expression were evaluated in fresh peripheral blood whereas cytokine production was evaluated in whole blood cell cultures stimulated with TLR agonists or Leishmania braziliensis antigen (Ag). CD16 monocytes frequency was increased in patients compared with controls.
View Article and Find Full Text PDFLeukotriene B modulation of murine dendritic cells affects adaptive immunity.
Prostaglandins Other Lipid Mediat
April 2019
Dendritic cells (DCs) link innate and adaptive immunity. The microenvironment generated during the innate immunity affects DCs and the type of adaptive immunity generated. Lipid mediators are released early in inflammation and could modify the functional state of DCs.
View Article and Find Full Text PDFMetacyclogenesis of : a comprehensive study of the main transformation features in axenic culture and purification of metacyclic promastigotes by negative selection with lectin.
Parasitology
May 2019
Article Synopsis
- Leishmania (Viannia) guyanensis is a parasite that causes skin infections in the Americas, but not many studies about it exist.
- Researchers have studied how this parasite changes and becomes ready to infect cells, finding that it takes about 4 days for it to become fully capable of causing infection.
- This research is the first to detail how the parasite matures in the lab and also created a way to separate its most infectious form, which can help understand and fight the disease better.
In vitro metacyclogenesis of Leishmania (Viannia) braziliensis and Leishmania (Leishmania) amazonensis clinical field isolates, as evaluated by morphology, complement resistance, and infectivity to human macrophages.
Biomed Res Int
November 2015
This study was designed to assess in vitro metacyclogenesis of Leishmania (Viannia) braziliensis and Leishmania (Leishmania) amazonensis clinical field isolates obtained from patient lesions (L. braziliensis IMG3 and PPS6m; L. amazonensis MAB6).
View Article and Find Full Text PDFAlthough Leishmania (Viannia) braziliensis is the most prevalent species that cause American tegumentary leishmaniasis (ATL), the immune response against this parasite has been poorly investigated. Upon activation, macrophages produce a series of pro-inflammatory molecules, including the lipid mediator leukotriene B4 (LTB4). LTB4 has been shown to enhance several macrophage functions, but its role in human macrophages is less known.
View Article and Find Full Text PDFBackground: Parasites of the Leishmania genus alternate between the flagellated extracellular promastigote stage and intracellular amastigotes. Here we report the characterization of a Leishmania isolate, obtained from a cutaneous leishmaniasis patient, which presents peculiar morphological features.
Methods: The parasite was cultured in vitro and characterized morphologically using optical and electron microscopy.