Publications by authors named "Ilan Goldstein"
Article Synopsis
- Genetic interactions can lead to different effects from the same DNA perturbation across different individuals, highlighting the complexity of how genes influence traits.
- Researchers used advanced DNA sequencing techniques to analyze over 8,000 genetic modifications in yeast and found that 460 genes showed variable fitness effects depending on the genetic background of different yeast strains.
- They identified 234 interacting genetic loci, including four central hubs that influence how different perturbations affect cellular processes, indicating that individual responses to genetic changes are shaped by a complex network of interactions rather than isolated effects.
Interactions between genetic perturbations and segregating loci can cause perturbations to show different phenotypic effects across genetically distinct individuals. To study these interactions on a genome scale in many individuals, we used combinatorial DNA barcode sequencing to measure the fitness effects of 7,700 CRISPRi perturbations targeting 1,712 distinct genes in 169 yeast cross progeny (or segregants). We identified 460 genes whose perturbation has different effects across segregants.
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- * The study involved tracking different genetic variants of the yeast Saccharomyces cerevisiae in mice to see how they persist across various organs over time.
- * Findings revealed that both general beneficial genes and specific organ-targeted genes are necessary for fungal survival, with a complex interplay of genetic factors influencing persistence in different organs, particularly contrasting effects between the brain and other organs.
Spontaneous and induced mutations frequently show different phenotypic effects across genetically distinct individuals. It is generally appreciated that these background effects mainly result from genetic interactions between the mutations and segregating loci. However, the architectures and molecular bases of these genetic interactions are not well understood.
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