[Cytogenetic aberrations in peripheral blood mononuclear cells in acute Lyme-borreliosis patients].

It is known that among biological mutagenic factors the cytogenetic aberrations can be induced by not only intracellular parasites like viruses, but also some extracellular infectious agents including bacteria, protozoas and helminthes. The object of this work was to study the cytogenetic status of acute Lyme borreliosis patients. It was found that the acute Lyme borreliosis patients as compared with the control group showed significantly higher frequencies of lymphocytes with chromosome instability in the peripheral blood mononuclear cell cultures.

[Effects of oil-refining microbes (genus Acinetobacter) on cytogenetical structures of human lymphocytes in cell cultures].

The objective of this study was to assess ability of oil-refining bacteria Acinetobacter calcoaceticus and A. valentis to induce karyopathological abnormalities and chromosomal aberrations in human lymphocyte cultures. It was found that the cultures infected with A.

[The role of mutation of gene cyp1A1 and benzapilene in cytogenetic changes of urinary tract epitheliocytes in oil industry workers employed in the oil fields of the North of West Siberia].

The examination of 477 oil industry workers and office personnel (control) employed in the oil fields of the North of Tomsk and Tyumen regions has detected increased number of epithelyocytes with micronuclei and an elevated urine level ofbenzapilene in workers employed in oil production. Especially pronounced changes of the above parameters were observed in men with mutant alleles Val of CYP1A1 gene. An enhanced mutation process in oil production workers may be due to a resultant action of different factors on human genome.

[Proliferative response of peripheral blood lymphocytes in relation to the rate of infestation in patients with opisthorchiasis].

The aim of the investigation was to evaluate the proliferative function of mononuclear cells after stimulation with antigens or mitogens in patients with varying rates of chronic mixed infestation with Opisthorchis and Metorchis. The proliferative response of mononuclear cells to specific parasitic antigens in patients with opisthorchiasis of high infestation rates was ascertained to be considerably suppressed as compared with that of these cells in patients with low infestation rates. In addition, severe mixed infestation was attended by a suppressed lymphoproliferative response not only to specific helminth antigens, but also to nonspecific mitogen and mycobacterial antigen.

[Cytokine production in relation to the rate of infestation in patients with chronic opisthorchiasis].

The aim of the investigation was to study the specific features of in vitro cytokine production in response to specific parasitic antigens, mycobacterial antigen, and nonspecific mitogens in patients with chronic opithorchiasis who had varying infestation rates. There was a considerable increase in the mononuclear cell production of interleukin-4 and interleukin-10 after specific Opisthorhis antigen stimulation with purified tuberculin derivate or the mitogen conconavalin A in opisthorchiasis patients having varying infestation rates as compared with appropriate parameters in healthy individuals. The production of these cytokines in opisthorchiasis patients with a high infestation rate (Group 2) was significantly higher than that in those with a low infestation rate (Group 1).

[Nitric monoxide and other indicators of free radical processes and antioxidant system in patients with mixed or monoinvasion of Opisthorhis and Metorchis].

As compared with healthy individuals, patients with mixed Opisthorhis and Metorchis invasion and/or Opisthorhis monoinvasion were found to have a considerable increase in the serum levels of nitric monoxide and malondialdehyde with the concurrent suppression of catalase activity and lipid antioxidant activity, which correlated with the blood accumulation of some toxic trace elements. The highest accumulation levels of toxic trace elements, as well as the indicators of free radical processes were detected in patients with the mixed invasion as compared with the Opisthorhis monoinvasion group, which appears to be directly related to the intensity of invasion.

[Therapeutic effect of mineral water of the Belokurikha spa resort on the level of antinuclear antibodies and chromosomal aberrations in synovial cells of patients with osteoarthrosis].

The objective of this study was chromosomal analysis and measurement of anti-nuclear antibody level in synovial cells of 28 patients aged between 35 and 50 years with knee joint osteoarthrosis before and after radon therapy at an equivalent doe of 280 mSv per treatment course. It was shown that the presence of osteoarthrosis was associated with a rise in the number of synoviocytes characterized by cytogenetic defects in the form of various chromosomal aberrations the frequency of which positively correlated with the number of anti-nuclear antibodies. Radon therapy brought about a decreases in the titers of antinuclear antibodies in synovial cells and the number of chromosomal aberrations in their nuclear apparatus.

[Increased accumulation of some trace elements in peripheral blood and bile of patients infested with Opisthorchis felineus (Rivolta, 1884) and Metorchis bilis (Braun, 1890)].

The invasion with Opisthorchis felineus (Rivolta, 1884) is known to be common in the Ob River region, West Siberia. These trematodes parasitize biliary tract of devinitive host (man or some species of animals). Other opisthorchiid species occurring in West Siberia, Metorchis bilis (Braun, 1890), has also been recorded recently as human parasite.

[Cytogenetic defects in lymphocytes of patients with chronic opisthorchiasis concurrent with Epstein-Barr virus persistence].

Sixty three patients with chronic opisthorchiasis were cytogenetically, serologically, and biochemically studied. Most patients with opisthorchiasis were found to have higher or high titers of antibodies to Epstein-Barr viral antigens. There was a direct correlation between the titers of antibodies to Epstein-Barr virus capsid antigen and the count of cytogenetically damaged cells.

[The role of the association of Opisthorchis felineus maritae with the Epstein-Barr virus in the cytogenetic sequelae of an opisthorchiasis infection].

The immunofluorescence analysis of Epstein-Barr virus (EBV) antigens in the body of Opisthorchis felineus (OF) helminths was carried out. It was found that EBV antigens located in eggs of helminth. Adding of OF antigens and/or EBV to the lymphocyte human cultures of healthy donors can induce a cytogenetic damage in cells.

[A decrease in the level of erythrocytes with micronuclei under the influence of pyrimidine and thiazolidine derivatives in the blood of persons who came under radiation exposure as a result of the accident at the Siberian Chemical Combine].

The authors have found that pentoxylum (pyrimidine derivative) and leucogenum (thyazolidine derivative) are capable or reducing the number of cells with micronuclei in the blood of people who suffered from the radiation accident at the radiochemical works of the Siberian chemical plant. The most effective decrease in the cells with micronuclei in adults was observed two weeks after treatment, while in children the same result was achieved with leucogenum on the third day.

[Relationship between the number of erythrocytes with micronuclei and immunologic status in humans].

26 persons, having high level of erythrocytes with micronuclei were distinguished among clinically healthy people. While examining their health changes were registered in the systolic and pulse pressures, number of T-suppressors and T-helpers, natural killers, level of immunoglobulins and immune complexes, phagocytosis activity and peroxidases reaction of the leukocytes. Increase in the level of the cytogenetic aberrative lymphocytes was observed in all the cases.

[Cytogenetic instability, the ability to produce interferon and immunoreactivity of blood lymphocytes in patients with schizophrenia].

In schizophrenia patients, the level of T-lymphocytes with cytogenetic aberrations is elevated, and the ability of these cells for PGA-blast-transformation and the interferon synthesis is reduced. The elevation of the lymphocytes ability for blast-transformation in the presence of the native preparation of DNA and for lysis of the erythrocytes proper is marked in these patients. The observed changes were especially marked in schizophrenia with an interrupted course.

[Applicability of micronucleus analysis for testing the aneuploid cell level in mouse bone marrow subjected to the action of different mutagenic factors].

The results of parallel studies in murine bone marrow of aneuploid cells' level and of micronuclei cells under the action of different mutagenic factors (X-irradiation, ethanol, measles virus and colchicine) are presented. The correlation analysis demonstrated the absence of correlation for the indices studied, in most cases. Possible reasons for this phenomenon are discussed.

[Use of the micronucleus test in the screening and monitoring of mutagens].

Data from literature on the use of micronuclear test to determine mutagenicity in agents of physical, chemical and biological nature are presented. The objects on which this method is used most frequently are enumerated. Great attention is paid to the analysis of micronuclei in blood erythrocytes and bone marrow of animals.

[The autoimmune process and chromosomal anomalies].

Data available in literature on the role of immune system in the cytogenetic stability of the organism are presented. A direct relation is shown to exist between immunity disturbances and changes in the number and structure of chromosomes.

Cytogenetic disturbances and immunoreactivity in patients with measles and influenza.

The number of lymphocytes with cytogenetic aberrations is increased in patients with measles and influenza. Considerable changes in immunoreactivity of the organism can be observed during measles and influenza. Correlation analysis suggests that in measles and influenza there exists an inversely proportional connection between the number and functional activity of T lymphocytes and the level of cytogenetic disturbances in them.

[Comparative characteristics of different methods of studying the cytogenetic disturbances in the bone marrow cells of Macaca rhesus monkeys infected with the poliomyelitis virus].

It has been testified that to study the influence of viruses on cytogenetic structures of bone marrow cells of monkeys (Macaca mulatta), it is possible to use not only the traditional methods, i.e. the analysis of chromosome set and the pathologic division, but the micronuclear test as well, based on the study of polychromatophylous erythrocytes with micronuclei in the bone marrow of animals.

[Role of Toxoplasma gondii in human and animal chromosomal pathology].

Toxoplasma gondii was found to cause an appearance of cells with a changed number of chromosomes in the bone marrow of infected white rats. Besides the increase of aneuploid and polyploid cells the infection with toxoplasms inhibits the mitotic activity in bone marrow of rats. The similar changes were observed in blood leucocytes of toxoplasmosis patients.

[Effect of ethanol and its metabolite acetaldehyde on the chromosomal apparatus of rat and human cells].

A higher level of cells with a changed number of chromosomes in leucocyte blood culture of chronic alcohol users (drunkards) and spermatogency cells of alcoholized rats has been noticed. Ethanol metabolite acetaldehyde exerts the same influence in the leucocyte blood culture of healthy donors. A prolonged influence of ethanol on rats, and also the effect of large doses of ethanol on the leucocyte blood culture of healthy donors cause an increase in the quantity of cells with chromosome translocations and gaps.