[Detection of pulmonary tuberculosis among the adolescent population at the stage of primary health care].

A retrospective look was made on the estimation of the diagnostic value of preclinical and clinical methods for verification of pulmonary tuberculosis. Among 170 examinees, 65.5% were self-directed, 24.

[Effect of elicitors on accumulation of protease inhibitors in injured potato tubers].

The time course of accumulation and the composition of proteinase-inhibiting proteins in diffusates from potato tubers treated with elicitors such as salicylic, jasmonic, and arachidonic acids were studied. The 40-kDa reserve protein patatin and the chymotrypsin inhibitors, among which proteins of 24.6, 22.

[Immunosuppressors in pathogenesis of potato-pathogen blight].

The properties and effects of two plant resistance suppressors (1,3-beta-1,6-beta-glucan and a pentasaccharide of xyloglucan origin) involved in the pathosystem of potato (Solanum tuberosum) and the causal agent of blight (Phytophthora infestans (Mont) de Bary) were compared. The microbial 1,3-beta-1,6-beta-glucan suppressed the defense response over a narrow concentration range (10(-2) M), whereas the plant pentasaccharide had a broad range of effective concentrations (10(-12) to 10(-6) M). In the pathosystem of potato-causal agent of late blight, the beta-glucan caused a local and race-specific suppressor effect on the plant host defense response.

[Effect of arachidonic acid on potato tubers during storage].

Potato (Solanum tuberosum L.) tubers were treated with various concentrations (10(-9) to 10(-4) M) of the biogenic elicitor arachidonic acid during the period of storage (from October to July). The data showed that the resistance-inducing concentration of arachidonic acid was 10(-6) M in autumn and 10(-9) M in spring.

[Modulation of plant resistance to diseases by water-soluble chitosan].

Low-molecular-weight water-soluble chitosan with a molecular weight of 5 kDa obtained after enzymatic hydrolysis of native crab chitosan was shown to display an elicitor activity by inducing the local and systemic resistance of Solanumi tuberosum potato and Lycopesicon esculentum tomato to Phytophthora infestans and nematodes, respectively. Chitosan induced the accumulation of phytoalexins in tissues of host plants, decreased the total content and changed the composition of free sterols producing adverse effects on infesters, activated chitinases, beta-glucanases, and lipoxygenases, and stimulated the generation of reactive oxygen species. The activation of protective mechanisms in plant tissues inhibited the growth of taxonomically different pathogens (parasitic fungus Phytophthora infestans and root knot nematode Meloidogyne incognita).

[Localization of proteins, immunologically similar to Eschericha coli RecA protein, in structures of cultured cells of HeLa and K-562 cell lines].

Earlier, polyclonal antibodies to the Escherichia coli RecA protein revealed immunologically related proteins only in the reproductive tissues of various eukaryotes and the proteins were localized to the various structures of the synaptonemal complex (SC). Indirect immunofluorescent FITC staining with polyclonal antibodies against RecA detected RecA-like antigens in the cell center (centrosome) and the replicated centrioles of fixed HeLa and K-562 cells. Human and mouse genes, which were previously cloned by immunological relatedness of their products to E.

[Cloning of a new murine gene coding for a protein immunologically related to RecA protein from Escherichia coli].

Using the testis-specific murine cDNA library immunoscreening with the affine-purified polyclonal antibodies to the E. coli RecA protein, the 1730-bp fragment of the novel mouse gene was cloned. Northern hybridization of total RNA samples from mouse somatic and meiotic tissues showed that this gene was specifically expressed in mouse testis, producing a transcript of about 10 kb in size.

[Effect of methyljasmononate on induction of late-blight resistance of potatoes using arachidonic acid].

Methyl ester of jasmonic acid (Me-JA) influences the induced resistance of potato tubers to late blight caused by Phytophthora infestans. Treatment of potato tuber disk surface with Me-JA solution or exposure to an atmosphere containing Me-JA vapors (10(-6)-10(-5) M) increased the rate of rishitin biosynthesis induced by arachidonic acid or P. infestans.

[Localization of RecA-like proteins in preparations of spread nuclei of mouse spermatocytes at meiotic prophase I].

Earlier, polyclonal antibodies to Escherichia coli RecA protein were used to identify immunologically related proteins in meiotic spermatocytes of different eukaryotes. At least one such protein proved to be a component of the synaptonemal complex (SC) [1]. Subsequent experiments on localization of RecA-like antigens in SCs of spermatocytes were performed by indirect immunocytochemical methods and electron microscopy, which showed that RecA-like protein(s) at early leptotene are largely associated with chromatin.

[Transposition of mobile dispersed genes (MDG) after substitution of various chromosome pairs in inbred Drosophila melanogaster strains].

Transpositions of MDG-1, MDG-3 and copia were detected as a result of crosses of the inbred maladaptive LA stock with laboratory stocks, in order to construct the genomes carrying different combinations of the LA or non-La chromosomal pairs. Changes of the mobile gene distributions were revealed in chromosomes of hybrid genotypes, as compared to parental chromosomal pairs. A trivial source of variability of chromosomal molecular structure ensured by crossing over was excluded by inversions which serve as suppressors of crossing over in corresponding crosses.