Anti-tumor activity of recombinant mouse tumor necrosis factor (TNF) on colon cancer in rats is promoted by recombinant rat interferon gamma; toxicity is reduced by indomethacin.

The activity and toxicity of rMuTNF, alone or combined with rat recombinant interferon gamma (rRIFN gamma), was tested in inbred WAG rats bearing a weakly immunogenic colon adenocarcinoma (CC531). The tumor was implanted s.c.

Continuous administration of pharmacological agents in heterotopically transplanted hearts.

A modification of the intraabdominal heterotopic heart transplantation model in rats is described which enables the local administration of pharmacological agents by means of a mini osmotic pump. In this modification, the aortic arch of the donor heart is cut after junction of the left carotid artery to enable the introduction of a catheter in the innominate artery. This catheter is connected to an implantable mini osmotic pump which delivers a continuous flow for 7 days.