Publications by authors named "Igor Volotovski"
The design of novel wound dressings for chronic wound treatment is still of great importance. One of the promising approaches is application of mesenchymal stem cells (MSCs), immobilized on a flexible polymer film, for healing. In this study, blended films based on polyvinyl alcohol (PVA) and pectin with different component ratio have been prepared by solution casting method and evaluated.
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- The study focused on creating ultrathin poly(L-lactic acid) (PLLA) films using a spin-coating technique and examined their physical properties like thickness and transparency.
- By adjusting polymer concentration and humidity, researchers were able to control the film's surface structure, producing either microporous or flat films.
- The resulting PLLA films supported the growth of mesenchymal stem cells and fibroblasts, demonstrated effective drug entrapment and release, making them promising materials for regenerative medicine in treating chronic wounds.
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- Layer-by-Layer assembled polyelectrolyte films utilize negatively charged biopolymers and positively charged polysaccharides to influence cell attachment and behavior on surfaces.
- The study focused on the properties of these films, particularly those containing chitosan, which exhibited linear growth, smooth surfaces, and viscoelastic characteristics, while films with polyethyleneimine showed increased rigidity and roughness.
- When tested with mesenchymal stem cells, chitosan films promoted effective cell adhesion and high viability, suggesting that the stiffness and surface properties of these films are crucial for tissue engineering applications.
• The drought hormone abscisic acid (ABA) is widely known to produce reductions in stomatal aperture in guard cells. The second messenger cyclic guanosine 3', 5'-monophosphate (cGMP) is thought to form part of the signalling pathway by which ABA induces stomatal closure. • We have examined the signalling events during cGMP-dependent ABA-induced stomatal closure in wild-type Arabidopsis plants and plants of the ABA-insensitive Arabidopsis mutant abi1-1.
View Article and Find Full Text PDFAmplification and a cloning of fragments of genes of human retina tyrosine kinases, the nucleotide sequences of which feature a high homology to the gene families of the Yes and Csk tyrosine kinases, and a cloning of the complete coding sequence of the cDNA of the Csk tyrosine kinase gene of the human lymphocytes have been carried out. It has been established that this sequence contains 1,624 bp and encodes a protein that, with a 99% homology, corresponds to the human tyrosine kinase. A comparative analysis of the nucleotide sequences of the full-size cDNA of the Csk tyrosine kinase of the lymphocytes of healthy donors and of patients with an eye choroidal melanoma has shown that a risk of development of an eye choroidal melanoma can be estimated by the frequency of occurrence of a mutant allele in the 10th exon.
View Article and Find Full Text PDFPhospholipase D (PLD) activity was found to be higher in etiolated oat seedlings than in green seedlings. White and red (R) light exposure inhibited PLD activity in etiolated seedlings. Far-red light eliminated R-light-induced decrease in PLD activity, indicating phytochrome participation in observed photomodulation.
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