Publications by authors named "Igor V Mizgirev"
CRISPR/Cas (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated protein) genome editing is a powerful technology widely used in current genetic research. In the most simple and straightforward way it can be applied for a gene knockout resulting from repair errors, induced by dsDNA cleavage by Cas nuclease. For decades, zebrafish () has been known as a convenient model object of developmental biology.
View Article and Find Full Text PDFThe efficacy of cyclophosphamide (CY), vincristine (VCR) and prednisolone (PRE) were studied in leukemia-bearing zebrafish larvae. A transplantable T-cell acute lymphoblastic leukemia (T-ALL) line ZL1 was induced by mosaic expression of zRag2-EGFP-mMyc transgene and underwent more than 20 consecutive transplantations in adult syngeneic fish prior to the experiments. Drug efficiency was assessed by an increase of lifespan (ILS) of treated leukemia-bearing animals as compared with untreated leukemia-bearing animals.
View Article and Find Full Text PDFArticle Synopsis
- Self-renewal is a critical characteristic of cancer that can be evaluated through cell transplantation in zebrafish, a model that previously lacked the necessary tools for such studies.
- Myc-induced T-cell acute lymphoblastic leukemias (T-ALLs) can be effectively created in zebrafish and transplanted into genetically similar siblings without immune suppression, revealing that self-renewing cells make up a significant portion of T-ALL mass.
- The research introduces high-throughput imaging techniques to facilitate the study of numerous transgenic zebrafish, allowing for extensive analysis of the gene pathways related to cancer self-renewal and the tumor-initiating potential of leukemias.