Publications by authors named "Igor Daniel de Miranda Lima"
Isolation and Characterization of the Growth Hormone (ag-GH) cDNA and Three-Dimensional Modeling of This Hormone in Comparison with the Human Hormone (hGH).
Eliana Rosa Lima Renan Passos Freire Miriam Fussae Suzuki João Ezequiel Oliveira Vanessa Luna Yosidaki Igor Daniel de Miranda Lima
Biomolecules
January 2023
In a previous work, the common gonadotrophic hormone α-subunit (ag-GTHα), the ag-FSH β- and ag-LH β-subunit cDNAs, were isolated and characterized by our research group from pituitaries, while a preliminary synthesis of ag-FSH was also carried out in human embryonic kidney 293 (HEK293) cells. In the present work, the cDNA sequence encoding the ag-growth hormone (ag-GH) has also been isolated from the same giant Arapaimidae Amazonian fish. The ag-GH consists of 208 amino acids with a putative 23 amino acid signal peptide and a 185 amino acid mature peptide.
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- The enzyme enoyl-ACP reductase (FabI) is crucial for fatty acid biosynthesis in bacteria and represents a potential target for new antibacterial drugs.
- Research using molecular dynamics reveals that the multimerization of FabI enhances the integrity of its catalytic site, with inhibitor binding promoting this process.
- Notably, the inhibitor AFN-1252 induces specific conformational changes and allows more water molecules to access the binding site, suggesting a new direction for developing FabI inhibitors.