Individual Differences in the Response of Human β-Lymphoblastoid Cells to the Cytotoxic, Mutagenic, and DNA-Damaging Effects of a DNA Methylating Agent, -Methylnitrosourethane.

Metabolic activation of many carcinogens leads to formation of reactive intermediates that form DNA adducts. These adducts are cytotoxic when they interfere with cell division. They can also cause mutations by miscoding during DNA replication.

Persistent parvovirus B19 infection in non-erythroid tissues: possible role in the inflammatory and disease process.

Parvovirus B19 (B19V) is a small non-enveloped DNA virus of the Parvoviridae family. It is an obligate human pathogen that preferentially replicates in erythroid progenitor cells. B19V is the causative agent of multiple erythroid-related diseases due to replication-induced cytotoxicity.

[Low level of 25-OH-vitamin D as a marker of critical ischemia in case of diabetic foot syndrome].

It was done the prospective study of the examination results of 40 patients with diabetes mellitus. All patients were divided into 2 groups for study of 25-OH-vitamin D effect, cholesterol, triglycerides in blood serum on the nature of diabetes mellitus. The first group included patients with ulcero-necrotic injuries in case of neuro-ischemic forms of diabetic foot syndrome.

Human parvovirus B19 infection leads to downregulation of thyroid, estrogen, and retinoid hormone receptor expression.

Erythrovirus B19 (B19V) is a member of the family Parvoviridae. Infection with B19V has been linked to a variety of diseases including erythroid, thyroid, neurological and autoimmune diseases. Here we show that infection of primary CD36+ cells with B19V coincides with downregulation of thyroid, retinoid, and estrogen hormone receptors.

[Peculiarities of arterial lesions in patients with critical ischaemia on the background of diabetic foot syndrome].

The authors investigated peculiarities of occlusive and stenotic arterial lesions influencing the pattern of revascularization in patients presenting with a neuroischaemic form of diabetic foot syndrome. We examined a total of 106 patients with a neuroischaemic form of diabetic foot syndrome and undergoing treatment at the City Municipal Centre for Diabetic Foot in the City of Minsk. In order to visualize the arteries in 82 (77.

[Revascularization surgery in the treatment of the diabetic foot syndrome].

The prospective study includes 142 patients with necrotic ulcers of feet in frames of the neuroiscemic form of the diabetic foot syndrome. 60 patents had revascularization procedures, of them 14 had open vascular operations and 46 had endovascular procedures. The high efficacy of revascularization in the group of patients with neuroiscemic form of the diabetic foot syndrome.

[Treatment of chronic critical ischemia by neuroischemic form of the diabetic foot syndrome].

Treatment results of 203 patients with critical ischemia by neuroischemic form of the diabetic foot sindrome were analyzed. 45 patients had open, endovascular or hybrid vascular reconstructions. The method was decised basing on the results of angiographic studies.

Effect of TNFalpha on activities of different promoters of human apolipoprotein A-I gene.

Human apolipoprotein A-I (ApoA-I) is a major structural and functional protein component of high-density lipoproteins. The expression of the apolipoprotein A-I gene (apoA-I) in hepatocytes is repressed by pro-inflammatory cytokines such as IL-1beta and TNFalpha. Recently, two novel additional (alternative) promoters for human apoA-I gene have been identified.

[Transfer of genetic constructions through the transplacental barrier into mice embryos].

Genetic modification of mammalian embryos is an important way to model various changes in human development; also, it is an instrument for studying the functions of certain genes in mammals. Using our own experience in developing modes of delivery of genetic constructions to mammals in a nonviral way, we present here data on the delivery of a eukaryotic expression vector to mice embryos through the transplacental barrier with the use of hydrodynamic intravenous injections of DNA-hybrid peptide complexes to pregnant females. The peptide has a cationic part for interaction with DNA and includes a ligand structure towards receptors of the releasing factor of luteinizing hormone (RFLH, luliberin).

[The role of ZF5 and CGGBP-20 transcription factors in expression regulation of human FMR1 gene responsible for X-fragile syndrome].

The human FMR1 gene encodes an RNA-binding protein taking part in translation regulation. The 5'-untranslated region of FMR1 gene contains a large number of tandem repeats of GCC triplets (5-50) which increasing (more then 200) is responsible for X-fragile syndrome (human congenital anomaly). As it has been shown earlier, al least two transcription factors (ZF5 and CGGBP-20) are capable of interacting specifically with GCC-repeats in regulatory regions of some genes.

[Effect of revascularization of on therapeutic outcomes of treatment for lower-limb chronic critical ischaemia in neuroischaemic form of diabetic foot syndrome].

The article deals with diabetic foot syndrome defined as a multidisciplinary problem. A reconstructive vascular operation in critical ischaemia on the background of diabetic foot syndrome is herein shown to be an efficient and justified intervention. Revascularization makes it possible to carry out sparing resections on the foot, to appropriately cope with the events of lower-limb critical ischaemia, and to reliably decrease the rate of high amputations.

[Thrombectomy in treatment of mesenteric vessels thrombosis].

The article presents two clinical case reports concerning successful surgical management of acutely impaired mesenteric haemocirculation accompanied and followed by the development of arterial and venous infarction of the large intestine.

Role of the zinc-finger and basic motifs of chrysanthemum virus B p12 protein in nucleic acid binding, protein localization and induction of a hypersensitive response upon expression from a viral vector.

The genomes of carlaviruses encode cysteine-rich proteins (CRPs) of unknown function. The 12 kDa CRP of chrysanthemum virus B (CVB), p12, has been shown previously to induce a hypersensitive response (HR) when expressed from potato virus X (PVX). This study demonstrated that a p12-induced HR was preceded by induction of a number of genes related to pathogenesis, stress and systemic acquired resistance.

[Ap1-like cis-elements in 5'-regulatory region of human apolipoprotein A-I gene].

Several Ap1-like cis-acting elements were found within 5'-regulatory region (-2497...

Novel repressor of the human FMR1 gene - identification of p56 human (GCC)(n)-binding protein as a Krüppel-like transcription factor ZF5.

A series of relatively short (GCC)(n) triplet repeats (n = 3-30) located within regulatory regions of many mammalian genes may be considered as putative cis-acting transcriptional elements (GCC-elements). Fragile X-mental retardation syndrome is caused by an expansion of (GCC)(n) triplet repeats within the 5'-untranslated region of the human fragile X-mental retardation 1 (FMR1) gene. The present study aimed to characterize a novel human (GCC)(n)-binding protein and investigate its possible role in the regulation of the FMR1 gene.

Complexes of DNA with cationic peptides: conditions of formation and factors effecting internalization by mammalian cells.

This work was devoted to the study of conditions of the formation of DNA/K8 complex and analysis of factors effecting the entry of DNA/K8 complex into mammalian cells in comparison with DNA complexes with arginine-rich fragment (47-57) of human immunodeficiency virus (type 1) transcription factor Tat (Tat peptide). The stoichiometry of positively charged DNA/K8 complexes has been studied for the first time. Non-cooperative character of DNA-K8 interaction was revealed.

Complexes of plasmid DNA with basic domain 47-57 of the HIV-1 Tat protein are transferred to mammalian cells by endocytosis-mediated pathways.

Arginine-rich peptides, penetratins, as part of a number of cellular and viral proteins, can penetrate across plasma membrane directly, without participation of endocytosis. We show that one of penetratins, the basic domain 47-57 of human immunodeficiency virus, type 1, transcription factor Tat (Tat peptide), is able to interact with plasmid DNA electrostatically. These interactions result in formation of polyelectrolytic complexes at various negative/positive charge ratios of plasmid DNA and Tat peptide.

[Role of the interferon system in the course of neglected forms of colorectal cancer].

Operations for neglected forms of colorectal carcinoma were performed on 66 patients. The interferon status was determined before the operation, on the 1st, 7th and 14th days after the operative intervention. "Viferon-2" was given to 18 patients (starting from 2 days before the operation) with the daily dose 1,000,000 IU.

[New surgical methods for treating hepatic hydatic cyst].

The authors have developed a set of instruments for endovideosurgical treatment of hydatid cysts of the liver. It was successfully used in operative ablation of the cysts by a traditional method in 9 patients, and in 7 patients laparoscopic echinococcus ectomy was performed without any complications.

[Procedures for the surgical treatment of cancer of the distal portions of the large intestine].

The authors observed 224 patients with neglected forms of carcinoma of distal portions of the colon, 171 (76.3%) of them being considered to be operable. The character of operative interventions depended on neglect of the tumour process which was determined by its spread to the neighbouring organs and tissues.