[New approaches to the study of viruses using scanning probe microscopy].

Atomic force microscopy was applied for studying individual virus particles. The micromechanism of rotaviruses was studied using the force curves of an atomic force microscope, with the Young's modulus of an individual particle being 1.0 x10(8) +/- 0.

[Use of atomic force microscopy for detecting intestinal viruses].

A possibility was demonstrated to use the atomic force microscopy in visualizing and identifying a variety of viruses contained in water samples by their morphological characteristics (shape and size), which was made by the example of 3 model objects, i.e. poliovirus, rotavirus and adenovirus.

[The effect of the amount of dialdehyde cellulose-bound enzyme on the activity of immobilized trypsin after gamma irradiation and in the storage process].

It is found the complex effect of the bound enzyme concentration on the proteolytic activity of trypsin immobilized to dialdehydecellulose (preriodate oxidation) after gamma irradiation and in process of storage. It is shown the occurrence of three stages of immobilized enzyme inactivation in process of immobilization and storage. The velocity of inactivation did not depend on bound trypsin concentration.

[The use of trypsin and lysozyme immobilized on a cellulose carrier in treating otitis externa].

The authors present a theoretical and practical validation of lysozyme+trypsin therapy for diffuse external otitis. A total of 32 patients were treated by a powder of trypsin immobilized on cellulose carrier as well as lysozyme and trypsin immobilized on gauze turundae. The treatment proved effective against bacterial and fungal external otitis.

[The effect of gamma radiation on the enzyme activity of collalitin during storage].

A study was made of the effect of gamma-irradiation of collalitin samples with doses of 25 and 50 kGy on proteolytic and enzyme activity with respect to denaturated collagen. The enzyme properties were found to change upon storage for periods of 6 and 12 months at 15 degrees C under unsterile conditions. No significant changes in the molecular mass of the collalitin samples were revealed by the method of gell-chromatography after irradiation and storage.

[Preparation of lysozyme immobilized on textile carriers and its use in treating suppurative wounds].

Immobilized forms of lysozyme were prepared by its covalent binding on dialdehyde cellulose and polycaproamide fibres as woven and knitted fabrics respectively. The preparations were estimated by the content of protein and bacteriolytic activity. The lysozyme activity per 1 g of the carrier and the protein content on dialdehyde cellulose were several times higher than those on polycaproamide while the specific activity of lysozyme on the polycaproamide carrier was somewhat higher than that on dialdehyde cellulose.

[Urinary glycosaminoglycan excretion in children born to diabetic mothers].

Glycosaminoglycan excretion with the urine was determined in 108 children born to mothers suffering from diabetes mellitus. In children with potential and latent diabetes, glycosaminoglycan excretion was increased as compared to children without diabetes mellitus aggravated heredity. The frequency of detection of increased glycosaminoglycan excretion in children did not depend on the length of the disease and its severity in mothers.

[Dynamics of erythrocyte hexokinase activity during glucose tolerance test in children with hereditary diabetes mellitus].

In determining the changes in hexokinase activity in erythrocytes during the glucose tolerance test in children with heredity aggravated by diabetes mellitus in comparison with such in apparently healthy children it was shown that in latent diabets the enzyme activity failed to alter during the whold period of study (on fasting stomach, 30, 60 and 180 minutes after glucose load), and increased 60 minutes after glucose load in potential diabetes, but to a lesser extent than in the control group. Changes of erythrocyte hexokinase response to glucose administration could serve as an auxiliary criterion for determination of the degree of risk in children with threatening diabetes.

[Effect of alpha-glycerophosphate on intracellular distribution of hexokinase in the tissue of fetal rabbits during acute hypoxia].

In experiments with 40 rabbit fetuses acute intrauterine hypoxia caused a redistribution of the hexokinase activity in brain and liver tissues towards an increase in the amount of the enzyme associated with mitochondria. Previous administration of alpha-glycerophosphate into rabbit female (at a dose of 1.3 g/kg of body weight) was accompanied by an increase in the hexokinase activity in liver mitochondria and in brain cytoplasmic fraction of fetus as well as in the total homogenate of both tissue.

[Dynamics of changes in hexokinase activity in subcellular tissue fractions of hypoxic newborn rats].

The activity of hexokinase (HK) in the total homogenate, cytoplasmic and mitochondrial fractions of the brain, heart and liver of the newborn rats was studied in relation to the severity of the hypoxic action. A mild from of hypoxic hypoxia caused in the tissue of the brain and liver an increase in the activity of mitochondrial-bound form of the enzyme with a simultaneous fall in the activity of the cytoplasmic HK; this was accompanied by a marked acceleration of glucose phosphorylation in the brain. Severe hypoxia led to the reduction of the HK activity in the total homogenate and both intracellular fractions in all the tissues under study.