[Feasibility study of perftoran for protection of the brain from ischaemia in operations on extracranial arteries].

Aim: To substantiate administration of <> for prevention of cerebral ischemia in operations on extracranial arteries.

Material And Methods: The present prospective randomized trial included a total of 50 patients having endured various operative interventions on extracranial arteries. Of these, 24 subjects were additionally given <> in order to prevent ischaemic complications.

Population profile of brain asymmetry in rats after intra-amniotic administration of vasopressin.

The effects of intra-amniotic administration of 1-desamino-8-D-arginine-vasopressin (DDAVP) at 14 days of embryogenesis on movement asymmetry in neonatal and mature mongrel white rats were studied. Controls consisted of intact and sham-operated animals, as well as rats given intra-amniotic saline. The population profile of asymmetry was assessed in terms of the tail position of rats aged two days, and also in terms of the direction of excursions in a T-maze in three-months males.

[Population profile of brain asymmetry in rats after intra-amniotic administration of vasopressin].

Vasopressin administration induced a right-sided bias in the rat litter, the asymmetry being similar to a right-sided bias in the turning behaviour of adult male rats in the T-maze. The findings suggest the nigrostriatal system to be responsible for the lateralising effect of vasopressin in the brain.

[The role of the maternal genotype in the realization of the embryotoxic activity of teratogens].

Against the background of the induced iron deficit ethanol (6.4 g/kg) causes aggravation of the embryolethal effect and anomalies in 15% of embryos in 14-day pregnant rats. Changes in the genome of rat males and females after the injection of the plasmid with a foreign gene at the stage of two pronuclei and the subsequent crossing with intact animals account for the increase in sensitivity of embryos to subteratogenic doses of sodium salicilate.

[An immunocytochemical analysis of the hormonal status of animals transgenic for growth hormone genes and for a mini-gene of human growth hormone-releasing factor].

An immunocytochemical analysis of hormonal status of transgenic rats containing human growth hormone gene has been done. The enhanced expression of the endogenous growth hormone gene was demonstrated with poly- and monoclonal antibodies inside somatatropes of pituitary. No activity of the heterologous growth hormone gene was revealed in kidney, pancreas or liver as it might be expected according to specificity of MT1 and TAT promotors.

[The stage-specific sensitivity of rat embryogenesis to ethanol administered intra-amniotically and by other methods].

The exposure of rat fetuses to ethanol by direct intra-amniotic injection in the afternoon of the 14th day of embryogenesis resulted in developmental anomalies (34%), the defects located mostly in the left extremities. Intraperitoneal injections of ethanol at the same period of pregnancy resulted in analogous injuries but only in occasional fetuses. There was no embryotoxic effect after exposure to high doses of ethanol by per os.

[The effect of amniotic fluid taken from women in labor on the morphofunctional development of fetal and newborn rats].

Amniotic fluid from women was injected intra-amniotically to rats at the 14th day of pregnancy in a dose 0.3 ml. Morphological and functional parameters of embryonic development of rats and EMG activity of rat puppies at the age of 30-34 days differed from control ones with respect to the degree of maturity and motor disturbances of newborn babies.

[The integration and expression of the beta-casein gene of Bos taurus in transgenic rats and mice].

Transgenic rats and mice carrying Bos taurus beta-casein gene were obtained using microinjection into the male pronucleus. Integration, inheritance and expression of the gene in transgenic animals were studied.

[The transformation of the mammalian genome and the teratogenic activity of chloridin].

Susceptibility to the effect of teratogen chloridine of three generations produced from transgenic rats with human growth hormone gene was investigated. The offsprings were shown to reveal elevated teratogenic injury as compared with intact animals.

[The viability of rat amd mouse embryos in low-temperature preservation].

Embryos of the laboratory rats and mice at the two-cell stage were frozen in different media with 1.5 M DMSO at a rate of 0.5-0.

[Immunobiological analysis of isolated subunits of influenza virus hemagglutinin].

The immunogenic properties of isolated subunits of influenza A/FM/1/47 (H1N1) virus hemagglutinin were studied. The antihemagglutinating serum was tested by the CFT, HI, competitive solid-phase radioimmunoassay, and biological neutralization test. The antiserum was found to contain populations of antibodies to the three main antigenic determinants of hemagglutinin identical to the determinants of the intact virion, as well as antibodies to the antigenic components of host cells.

[Immunological study of antigenic determinants in the isolated hemagglutinin of the influenza A virus (H1N1)].

A preparation of isolated subunits of influenza A (H1N1) virus hemagglutinin was examined for antigenic properties by four serological tests: radioimmunoassay, radial immunodiffusion test, complement fixation test, and hemagglutination inhibition test with hyperimmune polyclonal serum to intact virion as well as with monospecific antibodies to individual antigenic determinants obtained by adsorption technique. In isolated hemagglutinin subunits, radioimmunoassay identified three main virus-specific antigenic sites identical to antigenic determinants in the hemagglutinin of the intact virion. No neuraminidase was found in the hemagglutinin preparation but increased serological activity of host cell antigens was observed.

[3 cases of isolating the influenza A virus with human hemagglutinin Hsw1 in 1983 in Alma-Ata].

In the interepidemic period of late 1983 in Alma-Ata, influenza viruses with hemagglutinin of the swine subtype and neuraminidases N1 and N2 were isolated from humans. One of the strains, A/Alma-Ata/1044/83 (Hsw1N1), was isolated from the lungs of a man of 65 with the diagnosis of influenza and disseminated intravascular syndrome who had had communal and occupational contacts with swine. In paired sera of a sick child, and in seroepidemiological screening for influenza antibodies to swine influenza virus in low titres were detected in 34% of cases in subjects under 40.

[Detection and identification of the influenza virus in nasopharyngeal washings by solid-phase radioimmunological analysis].

In the period of epidemic rise of the morbidity level in 1983-1984, 207 specimens of nasopharyngeal washings from patients with influenza were analysed by solid-phase radioimmunoassay. Test sera containing a wide range of antihemagglutinating antibodies to different drift variants of influenza type A and type B viruses were used as diagnostic ones. The results indicate the predominance of influenza B infection (18.

[Identification of influenza virus type A by solid-phase radioimmunological analysis].

High sensitivity and specificity of solid-phase radioimmunoassay (SPRIA) in identification of influenza A (H1N1 and H2N2) viruses in the infected allantoic culture were demonstrated. Mixtures of influenza hyperimmune sera free from antibodies to host cell antigens and antineuraminidase antibodies were used as active test sera. The test serum a-H1N1 consisted of antisera to A/FmI/47, A/Netherlands/36/56, and A/USSR/090/77 strains; it detected practically all tested variants of H1N1 virus isolated in 1947-1982 in allantoic cultures containing virus-specific protein in amounts of 1.

[Decrease in the ascorbic acid content of guinea pig tissues caused by thalidomide].

It has been demonstrated that thalidomide is capable of a noticeable stabilization of the enzymes from rabbit, pig and guinea-pig liver microsomal fractions. Administration of thalidomide to guinea-pigs resulted in an increase of microsomal hydroxylase activity measured by the aniline test. The growth of the activity of microsomal enzymes led to an abrupt drop of ascorbic acid concentration in guinea-pig organs.

[Changes in rat behavior after glutethimide exposure in various stages of antenatal neurogenesis].

After administration to rats in a dose of 200-400 mg/kg on days 14 and 15 of the pregnancy the hypnotic drug glutethimide induces in the rat progeny variations in the orienting-motor activity, latent period of runnings and raises the frequency of urinations upon training in a maze. The effect of glutethimide on the orienting-motor behavior was seen only in the first generation of the animals and was absent from the second generation animals.

[Embryotoxic effect of ethanol and its biotransformation products in cultures of postimplantation rat embryos].

A study was made of the effect of ethanol (6.5-108 mM) and its immediate metabolite, acetaldehyde (4.5 microM-45 mM) on 9.

[In vitro development of the postimplantation embryos of laboratory rodents in human blood serum].

Rat and mouse embryos at the stage of the first somites formation (1-5 pairs) cultivated in human blood serum demonstrated its embryolethal and teratogenic effect. The embryos taken at a later stage (11-18 pairs of somites) developed normally and could be compared with the development of the rat embryos in homologous blood serum. There was no difference in the development when the embryos were cultivated either in male or female blood serum.