Pilot-scale microalgae cultivation and wastewater treatment using high-rate ponds: a meta-analysis.

Microalgae cultivation in wastewater has been widely researched under laboratory conditions as per its potential to couple treatment with biomass production. Currently, only a limited number of published articles consider outdoor and long-term microalgae-bacteria cultivations in real wastewater environmental systems. The scope of this work is to describe microalgal cultivation steps towards high-rate algal pond (HRAP) scalability and identify key parameters that play a major role for biomass productivity under outdoor conditions and long-term cultivations.

Microalgal consortium tolerance to bisphenol A and triclosan in wastewater and their effects on growth, biomolecule content and nutrient removal.

Amongst the many treatments available for the removal of emerging contaminants in wastewater, microalgal cultures have been shown to be effective. However, the effectiveness of exposure of a native microalgal consortium to emerging contaminants such as bisphenol-A (BPA) and triclosan (TCS) to determine the half-maximum effective concentrations (EC) has not yet been determined. The effect on growth and nutrient removal of such a treatment as well as on the production of biomolecules such as carbohydrates, lipids, and proteins are, at present, unknown.

Harvesting microalgae using ozone-air flotation for recovery of biomass, lipids, carbohydrates, and proteins.

The objective of this research was to study a novel ozone-air flotation microalgae harvesting method and evaluate its effect on the recovery of biomass and biocomponents (lipids, carbohydrates, proteins). Best processing conditions were established using a response surface methodology (RSM). Microalgae separation and biocomponent recovery were evaluated according to changes in gas concentration (13, 18 and 25 mgO/L), ozone dose (0.

Wastewater-leachate treatment by microalgae: Biomass, carbohydrate and lipid production.

Increases in wastewater discharges and the generation of municipal solid wastes have resulted in deleterious effects on the environment, causing eutrophication and pollution of water bodies. It is therefore necessary to investigate sustainable bioremediation alternatives. Wastewater treatment using consortia of microalgae-bacteria is an attractive alternative because it allows the removal and recycling of nutrients, with the additional advantage of biomass production and its subsequent conversion into valuable by-products.

Detection of VBNC Vibrio cholerae by RT-Real Time PCR based on differential gene expression analysis.

The recognition of the viable but non-culturable (VBNC) state of pathogenic bacteria has brought with it many questions to answer related to the need to detect and quantify viable bacteria in the environment in an accurate way. To assess viability of Vibrio cholerae, we developed a RT-Real Time PCR technique based on differential expression analysis from mRNA deep sequencing data. We compared two induction conditions to achieve the VBNC state: a bacterial suspension induced by artificial seawater at 4°C, and the addition of 3',5'-cyclic diguanylic acid.

Comparison between coagulation-flocculation and ozone-flotation for Scenedesmus microalgal biomolecule recovery and nutrient removal from wastewater in a high-rate algal pond.

The removal of nutrients by Scenedesmus sp. in a high-rate algal pond, and subsequent algal separation by coagulation-flocculation or flotation with ozone to recover biomolecules, were evaluated. Cultivation of Scenedesmus sp.

Biodiesel production from indigenous microalgae grown in wastewater.

This paper describes a process for producing biodiesel sustainably from microalgae grown in wastewater, whilst significantly reducing the wastewater's nutrients and total coliform. Furthermore, ozone-flotation harvesting of the resultant biomass was investigated, shown to be viable, and resulted in FAMEs of greater oxidation stability. Desmodesmus sp.

Determination of field capacity of municipal solid waste with surcharge simulation.

The following study was carried out as part of the environmental monitoring of a landfill in Nuevo Laredo, Tamaulipas (Mexico). The parameter of field capacity is important in predicting the amount of leachate generated by solid waste disposal sites, because of the polluting potential of leachate. This paper describes how the field capacity for municipal solid waste was determined, and the purpose of this document is to present a methodology, and to describe the devices designed for determining the field capacity of municipal solid waste.

Serial water balance method for predicting leachate generation in landfills.

This paper presents the "Serial Water Balance" method for predicting leachate generation in landfills. This procedure makes it possible to calculate the total leachate likely to be generated, by estimating an individual cell by cell water balance. This new development considers the interaction effects between cells, through the execution of simple field capacity tests on solid waste samples under different loading conditions.