Methodology for processing mastectomy and cryopreservation of breast cancer tissue in a resource- poor setting: A pilot study.

Background: There is scarcity of breast cancer tissues derived from women of African origin available for patient - derived xenograft and organoid models.

Objective: We aim to create a versatile protocol for processing mastectomy and cryopreservation of breast cancer tissue.

Methodology: An immediate collection of breast cancer tissue from mastectomy was bathed in 4 °C HBSS and immediately transferred to 4 °C RPMI1640 containing HEPES, 10% FBS, Streptomycin and Penicillin.

Boundary Associated Long Noncoding RNA Mediates Long-Range Chromosomal Interactions.

CCCTC binding factor (CTCF) is involved in organizing chromosomes into mega base-sized, topologically associated domains (TADs) along with other factors that define sub-TAD organization. CTCF-Cohesin interactions have been shown to be critical for transcription insulation activity as it stabilizes long-range interactions to promote proper gene expression. Previous studies suggest that heterochromatin boundary activity of CTCF may be independent of Cohesin, and there may be additional mechanisms for defining topological domains.