Publications by authors named "Idriss Ben Neila"
Article Synopsis
- Karlodinium veneficum is a harmful dinoflagellate that affects both human health and the environment, necessitating early detection for monitoring.
- A new real-time PCR method was developed to specifically detect and quantify K. veneficum, showing high specificity and sensitivity.
- The accuracy of this qPCR method was confirmed against environmental samples, making it a potentially useful tool for studying the presence and seasonal patterns of this species in marine ecosystems.
Gymnodinium catenatum is a dinoflagellate known to cause paralytic shellfish poisoning (PSP), commonly associated with human muscular paralysis, neurological symptoms, and, in extreme cases, death. In the present work, we developed a real-time PCR-based assay for the rapid detection of the toxic microalgal species, G. catenatum, in environmental bivalve mollusc samples as well as seawater samples.
View Article and Find Full Text PDFProrocentrum lima (P. lima) is a widely spread dinoflagellate in the Mediterranean Sea and it has become increasingly involved in harmful algal blooms. The purpose of this study is to develop a probe-based real-time polymerase chain reaction (PCR) targeting the ITS1-5.
View Article and Find Full Text PDFQuantitative PCR assay for the simultaneous identification and enumeration of multiple Karenia species.
Environ Sci Pollut Res Int
October 2020
Quantitative PCR (qPCR) is the method of choice for specific detection and quantification of harmful algal bloom (HAB) species. Development of qPCR assay for simultaneous enumeration of species that frequently co-exist in HABs is required. A high sensitivity TaqMan qPCR assay, using probe and primers, located at ITS1-5.
View Article and Find Full Text PDFElimination of and in Clam by Using Zeolite in a Station of Depuration.
Water Environ Res
September 2017
Article Synopsis
- - Natural zeolite is being researched for its effectiveness in treating water and wastewater, with potential applications in improving clam purification processes.
- - The study specifically focuses on using natural zeolite to reduce Escherichia coli and eliminate Salmonella in clams contaminated with these bacteria.
- - Results indicated that natural zeolite significantly enhances the clam purification process, reducing E. coli levels in just 24 hours and meeting the ISO 16649-3 standards for purified clams.
The objective of this study was to develop and evaluate a SYBR Green real time PCR method for the specific detection of Salmonella spp using a novel target, the siiA gene. Primer specificity testing was done on a panel of 76 Salmonella strains and 32 non-Salmonella strains. The primers directed against the siiA gene amplified all Salmonella strains tested, while non-Salmonella strains were not amplified.
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