Topical fluoride varnish application shifts dysbiotic dental plaque microbiome towards eubiosis in children with dental caries.

Objective: This study used high-throughput amplicon sequencing to examine the impact of long-term continuous fluoride treatment on the dental plaque microbiota of children aged 8 to 9 with mixed dentition.

Design: The study population consisted of twenty 8-9-year-old children with dental caries. Topical application of fluoride-varnish was weekly administered for one month to all subjects.