High frequency of anti-Saccharomyces cerevisiae antibodies in chronic hepatitis C.

Background And Study Aim: Chronic hepatitis C (CHC) is a liver disease caused by the hepatitis C virus. Anti-Saccharomyces cerevisiae (S. cerevisiae) antibodies (ASCA) are frequently reported in autoimmune diseases but rarely in viral infections.

Serological screening for celiac disease, by endomysial antibodies, in patients with rheumatoid arthritis.

Celiac disease (CD) and rheumathoid arthritis (RA) are both multi-factorial chronic inflammatory auto-immune diseases. In this retrospective study, we determined the frequency of CD in patients with RA using IgA anti-endomysial antibodies (EmA) and tried to explain this association. Indirect immunofluorescence on human umbilical cord was used to detect EmA in 215 patients with seropositive RA collected over a 4-year-period.

IgA is the predominant isotype of anti-β2 glycoprotein I in patients with COVID-19.

Objective: The aim of this research was to determine the frequency of antiphospholipid antibodies (aPL) in patients with COVID-19.

Methods: The frequency and titers of anticardiolipin antibodies (aCL) and anti-β2 glycoprotein I antibodies (aβ2GPI) were determined in sera of adult patients hospitalized with COVID-19. Immunoglobulin (Ig)G, IgA, IgM aCL, and aβ2GPI were measured using enzyme-linked immunosorbent assay.

Frequency of serological markers of rheumatoid arthritis in patients with Hashimoto's thyroiditis.

Background: Hashimoto's thyroiditis (HT) is an autoimmune disease that is frequently associated with other autoimmune conditions.

Objective: To perform serological screening for rheumatoid arthritis (RA) in patients with HT.

Methods: Our study included 88 consecutive serum specimens of patients with confirmed HT and 88 sex- and age-matched healthy subjects.

Anti-cardiolipin and anti-beta 2 glycoprotein I antibodies in patients with type 1 diabetes.

To determine the frequency of anti-phospholipid antibodies (aPL) in particular anti-cardiolipin antibodies (aCL) and anti-beta 2 glycoprotein I antibodies (aβ2GPI) in Tunisian patients with type 1 diabetes. One hundred and two patients with type 1 diabetes (34 children, 68 adults) were studied. As control groups, we used sera of 156 adults and 65 children without type 1 diabetes.

Frequency of antithyroid antibodies in patients with primary biliary cholangitis.

Objective: Primary biliary cholangitis (PBC) is an autoimmune disease of liver that may be associated with other conditions, including autoimmune thyroid diseases. We aimed to investigate the frequency of anti-thyroperoxidase antibodies (TPO-Ab), antithyroglobulin antibodies (TG-Ab), and anti-thyrotropin receptor antibodies (TSHR-Ab) in Tunisian patients with PBC.

Methods: Sera of 80 patients with PBC were collected over a 9-year period.

Serological markers of rheumatoid arthritis in patients with primary biliary cholangitis and the vice versa: A Tunisian study.

Background: Primary biliary cholangitis (PBC) is an autoimmune disease of the liver characterized by destructive lymphocytic cholangitis and anti-mitochondrial antibodies (AMA). Anti-gp210 and anti-Sp100, are used for the diagnosis of PBC in AMA-negative PBC patients. Patients with PBC have a propensity to have an extrahepatic manifestation which is especially autoimmune.

Positive myositis-specific autoantibodies during COVID-19: a case report.

Viral infection is known to be a trigger of autoimmune diseases. Numerous cases of coronavirus disease 2019 (COVID-19) with autoimmune manifestations have been reported and several authors have highlighted the relationship between severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and autoimmune diseases. Autoimmune myopathies being one of these manifestations.

Anticardiolipin and anti-beta 2-glycoprotein I antibodies in patients with unexplained articular manifestations.

Objective: To determine the frequency of antiphospholipid antibodies (aPL) in patients with unexplained articular manifestations.

Material And Methods: Three hundred thirteen patients suffering from arthritis or arthralgia without evident cause and 266 healthy blood donors (HBD) were included in the study. Anticardiolipin antibodies (aCL) and anti-beta 2-glycoprotein I antibodies (aβ2GPI) were measured by ELISA.

Antiphospholipid antibodies in autoimmune thyroid diseases.

Background: Antiphospholipid (aPL) antibodies have been reported in several autoimmune diseases. The aim of this study was to evaluate the frequency of aPL (anti-cardiolipin antibodies (aCL) and anti-β2 glycoprotein I antibodies (aβ2GPI)) in patients with autoimmune thyroid diseases (AITD).

Methods: One hundred and ninety-five patients with AITD (139 Hashimoto's thyroiditis (HT) patients and 56 Graves' disease (GD) patients) and 90 healthy blood donors (HBD) were studied.

Anti-Saccharomyces cerevisiae Antibodies in Rheumatoid Arthritis.

Objective: This study was conducted to evaluate the frequency of anti-Saccharomyces cerevisiae antibodies (ASCA) in patients with rheumatoid arthritis (RA).

Methods: Eighty-three RA patients with positive anti-cyclic citrullinated antibodies (anti-CCP) and 160 healthy blood donors were included in this study. ASCA IgG and IgA were assessed with enzyme-linked immunosorbent assay.

Frequency of serological markers of rheumatoid arthritis in patients with IgA anti-β2 glycoprotein I antibodies.

Aim: To determine the frequency of serological markers of RA in patients with anti-β2 glycoprotein I antibodies (aβ2GPI) of IgA isotype.

Material And Methods: A retrospective study was conducted on 67 patients with aβ2GPI-IgA. Ninety healthy blood donors (HBD) were used as a control group.

IgA anti-beta-2 glycoprotein I antibodies in chronic hepatitis C.

Background And Study Aims: Antiphospholipid antibodies (aPL) have been reported not only in various autoimmune conditions but also in other infections, such as chronic hepatitis C (CHC) infection. The aim of this study is to evaluate the frequency of aPL in patients with CHC.

Patients And Methods: Ninety-six CHC patients and 90 healthy blood donors (HBD) were studied.

Frequency of serological markers of rheumatoid arthritis in adult patients with active celiac disease.

Background: Celiac disease (CD) and rheumatoid arthritis (RA) are multisystem autoimmune diseases affecting 1% of general populationa. Both diseases share genetic and immunological features.

Aim: In this retrospective study, we aim to determine the frequency of auto-antibodies of RA in adult patients with CD.

Frequency of auto-antibodies of type 1 diabetes in adult patients with celiac disease.

Unlabelled: Both celiac disease (CD) and type 1 diabetes (T1D) are autoimmune diseases resulting from a complex interplay between genetic susceptibility and environmental factors.

Aim: In this retrospective study, we determined the frequency of auto-antibodies of T1D in adult patients with active CD.

Materials And Methods: Eighty adult patients with active CD were included in our study.

Anti-gp210 and anti-Sp100 antibodies in primary biliary cholangitis.

Background And Study Aims: To determine the sensitivity and specificity of anti-gp210 and anti-Sp100 autoantibodies in primary biliary cholangitis (PBC) PATIENTS AND METHODS: Sera of 106 PBC patients with positive anti-mitochondrial antibodies and 58 healthy blood donors were analyzed. A line immunoassay was used to evaluate the reactivity of anti-gp210 and anti-Sp100 antibodies.

Results: The frequency of anti-gp210 and anti-Sp100 autoantibodies was 29.

IgA is the predominant isotype of anti-β2 glycoprotein I antibodies in rheumatoid arthritis.

Background: The aim of this study was to determine the frequency of anti-cardiolipin antibodies (aCL) and anti-β2 glycoprotein I antibodies (aβ2GPI) among Tunisian patients with rheumatoid arthritis (RA).

Methods: Ninety RA patients with positive anti-cyclic citrullinated antibodies (anti-CCP) and 90 healthy blood donors (HBD) were studied. aCL and aβ2GPI of isotype IgG, IgA and IgM were detected by ELISA.

Screening for celiac disease, by endomysial antibodies, in patients with unexplained hypertransaminasaemia.

Objective: To do a serological screening for celiac disease in patients with unexplained liver cytolysis.

Materials And Methods: Fifty-six patients with liver cytolysis without known aetiology were studied. Endomysial antibodies were determined by indirect immunofluorescence on human umbilical cord.

Thrombopoietin Secretion by Human Ovarian Cancer Cells.

The thrombopoietin (TPO) gene expression in human ovary and cancer cells from patients with ovarian carcinomatosis, as well as several cancer cell lines including MDA-MB231 (breast cancer), K562 and HL60 (Leukemic cells), OVCAR-3NIH and SKOV-3 (ovarian cancer), was performed using RT PCR, real-time PCR, and gene sequencing. Human liver tissues are used as controls. The presence of TPO in the cells and its regulation by activated protein C were explored by flow cytometry.

Human regulatory B cells control the T cell response.

Background: Follicular helper T (T) cells support terminal B-cell differentiation. Human regulatory B (Breg) cells modulate cellular responses, but their control of T cell-dependent humoral immune responses is unknown.

Objective: We sought to assess the role of Breg cells on T cell development and function.

Anti Saccharomyces cerevisiae Antibodies in Patients With Anti-β2 Glycoprotein I Antibodies.

Background: In this study, cross-reactive epitopes on β2 glycoprotein I and Saccharomyces cerevisiae have been described. The objective of our study was to determine the frequency of anti S. cerevisiae antibodies (ASCA) in patients with anti-β2 glycoprotein I antibodies (aβ2GPI).

Endothelial protein C receptor gene 6936A/G single-nucleotide polymorphism as a possible biomarker of thrombotic risk in acute myeloid leukemia.

Protein C (PC) is a natural anticoagulant, which interacts with the endothelial PC receptor (EPCR). EPCR single-nucleotide polymorphism (SNP) 6936A/G results in high levels of a free soluble form of EPCR (sEPCR) and may affect the risk of coagulation. The objective of this study was to assess whether the 6936A/G SNP of the EPCR gene is involved in the procoagulant activity displayed by hematological malignancies.

High frequency of antiphospholipid antibodies in primary biliary cirrhosis.

Aim: To evaluate, retrospectively, the frequency of autoantibodies of antiphospholipid syndrome (APLS) in Tunisian patients with primary biliary cirrhosis (PBC).

Patients And Methods: We analyzed 80 PBC sera and 80 sera from blood donors. ELISA was used to determine the frequency of antibodies against cardiolipin (aCL IgG, IgA, and IgM) and beta 2 glycoprotein I (aβ2GPI IgG, IgA, and IgM).

Screening for celiac disease, by endomysial antibodies, in patients with unexplained articular manifestations.

Celiac disease (CD) is an autoimmune systemic disease characterized by not only gastrointestinal but also extraintestinal manifestations. The aim of our study was to do a serological screening for CD, by IgA endomysial antibodies (EmA), in patients with unexplained articular manifestations. Two hundred and eleven patients suffering from arthritis or arthralgia without evident cause were studied.