Quantification of furosemide in camel plasma by high resolution mass spectrometry, application on pharmacokinetics.

We developed and validated a high-resolution liquid chromatography mass spectrometry method for the quantification of furosemide in camel plasma which was used for a pharmacokinetic study in camels. Plasma samples were extracted by supported liquid extraction and furosemide and internal standard (furosemide-D5) were separated on a an Agilent Zorbax XDB C column (50 × 2.1 mm i.

Pharmacokinetics and metabolism study of firocoxib in camels after intravenous administration by using high-resolution bench-top orbitrap mass spectrometry.

In this study, we developed a high-resolution liquid chromatography mass spectrometry method for the pharmacokinetic study of firocoxib followed by full method validation. Following a solid-phase extraction, the firocoxib and internal standard (celecoxib) were separated on an Agilent Zorbax ZDB C18 column (50 mm × 2.1 mm i.

Trace determination of β-blockers and β2-agonists in distilled and waste-waters using liquid chromatography-tandem mass spectrometry and solid-phase extraction.

A highly sensitive method for simultaneous determinations of eleven β-blockers and β-agonists in distilled and waste-waters using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS-MS) was developed, optimized and validated. The method was used for trace determinations of acebutolol, atenolol, metoprolol, propranolol, timolol, nadolol, labetalol, oxprenolol, pindolol, alprenolol and terbutaline. Oasis MCX and Clean Screen cartridges were used for solid phase extractions and an alkaline mixture of dichloromethane-propanol was used as mobile phase.

Elimination and phase II metabolism of ethanol in camels after intravenous administration.

Ethanol elimination was studied in camels (n=8) after a single bolus intravenous dose of 0.1g/kg bodyweight (BW). Blood samples were then collected at set intervals.

A fatal case of oleandrin poisoning.

The study presents a case of fatal poisoning with oleander leaves in an adult diabetic male. After repeated vomiting, and gastrointestinal distress the patient was admitted at the hospital with cardiac symptoms 1h after the ingestion. Urine samples were assayed immunochemically and by GC-MS for drugs of abuse and for general toxicological screen.

Rapid and sensitive static headspace gas chromatography-mass spectrometry method for the analysis of ethanol and abused inhalants in blood.

A sensitive and specific method using static headspace gas chromatography coupled with mass spectrometry (GC/MS) has been developed for the quantitative determination of ethanol in biological fluids using n-propanol as internal standard. Gas chromatography was performed in isothermal mode with a GC run time of 2.6 min.