[Validation of an ELISA assay for the quantification of antibodies against Haemophilus influenzae type b capsular polysaccharide].

The validation study of an ELISA assay -internationally accepted for this purpose- was conducted aimed at having a method for the quantification of antibodies against Haemophilus influenzae type b (Hib) capsular polysaccharide in the clinical trials used to prove the immunogenicity of a new conjugated vaccine composed of a synthetic antigen. The validation was carried out in the National Haemophilus Reference Laboratory, in "Pedro Kouri" Institute of Tropical Medicine, Havana, Cuba. A protocol was designed, in which the determination of accuracy, exactness, linearity and limit of the detection of the assay were considered.

[Methicillin-resistant Staphylococcus aureus. Detection of carriers among hospitalized children and healthy children from the community].

A descriptive punctual study was conducted to search for nasal carriers of Staphylococcus aureus strains resistant to methycilin (SARM) among sound children attended in nurseries and hospitalized children exposed to one or more risk factors predisposing to the colonization by this type of strains from October, 2000, to February, 2001, in Marianao municipality. For the primary isolation of the SARM strains, it was used the oxacillin-manitol-salt-agar (OMSA) and the sensitivity to antimicrobial drugs was determined in all strains identified as S. aureus by using the Kirby-Bauer method.

Naturally acquired immunity to Haemophilus influenzae type B in healthy Cuban children.

We have evaluated the prevalence of antibody to immunogenicity of Haemophilus influenzae type b (Hib) in a group of 4 to 5 years old healthy children, who were too old to be included in the first vaccinated cohort when Hib vaccination begun in Cuba in 1999. Serum capsular polysaccharide specific IgG antibody concentrations were measured in 974 healthy children, between February and May 2002. The prevalence of Hib nasopharyngeal carriage was also estimated.