Possibilities of Endovascular Hemostasis in Treatment of Pancreatic Bleeding.

Pancreatic hemorrhage is one of the most severe complications of various pancreatic diseases that are difficult to treat even in multidisciplinary hospitals. Mortality from pancreatic hemorrhage can reach up to 80%. This study aimed to evaluate the possibility of maintaining endovascular homeostasis in the treatment of patients with pancreatic hemorrhage.

[A new approach for study of structural and functional properties of proteins with unknown functions].

Selected proteins were produced in Escherichia coli bacterial expression system--three proteins from extremophil bacteria: a putative monooxygenase from Deinococcus radiodurans, a putative nucleotidyltransferase from Thermotoga maritima, a putative oxidoreductase from Exiguobacterium sibiricum; and a shaperon from Homo sapiens DJ-1. The protocol of isolation & purification of recombinant proteins were developed that allowed to obtain expression products with the purity of no less than 96%. Conditions for the crystallization have been selected that allowed a stable growth of crystals.

[Prediction of acute biliary pancreatitis].

Authors propose mathematical model for prediction of acute biliary pancreatitis in patients with strangulated concrements of papilla Vateri. Values of α-amylase and lipase in blood and bile are used as main prognostic signs. It is estimated that blood lipase, bile α-amylase and lipase are main prognostic signs for development of acute biliary pancreatitis.

[Endoscopic transmural and transpapillar procedures for the treatment of chronic pancreatitis].

93 patients with chronic pancreatitis were treated endoscopically: 44 had transpapillar procedures and 49 patients had transmural operations. The transmural procedures were applied in case of pancreatic pseudicysts, whereas transpapillar endoscopic operations were applied in cases of the obstructive jaundice, pseudocyst, connected of the main pancreatic duct, virsungolithyasis or ductal pancreatitis with pain syndrome. The endoscopic procedures by chronic pancreatitis proved to be the reliable alternative to the traditional surgery.

[The possibilities of application of iodinedicerinum in urgent surgery].

Application of ioddicerynum while performance of an urgent operative intervention in 63 patients with an acute appendicitis, perforated gastroduodenal ulcer and an old postoperative abscess of anterior abdominal wall had permitted to lower the occurrence frequency for postoperative purulent complications by two times.

[The effect of sanatorium-health resort treatment on the function of the bactericidal oxidative mechanism of the blood neutrophils in patients with different forms of chronic bronchitis].

The authors' studies show that sanatorium treatment contributes to normalization of bactericidal oxidant mechanism (BOM) of blood neutrophils in patients with chronic bronchitis. In a complete remission this normalization is complete, in partial remission positive trends were seen. In chronic obstructive bronchitis the effect was minor.

[The importance of the cytochemical diagnosis of the inflammatory process in the lungs for the treatment and rehabilitation of children with recurrent bronchitis].

It has been shown that common clinical and paraclinical methods of diagnosis of recurring bronchitis in children fail to detect subclinical activity of the inflammatory process in the lungs, which fact poses rather difficult problem of treatment and rehabilitation in such pediatric patient populations. Cytochemical diagnosis relying on values for activity of acid phosphatase and labilization of membranes of blood multinuclear leucocytes lysosomes permits overcoming the above difficulties, enhancing efficiency of the treatment.

[The problems and prospects of a climatotherapy health resort under current conditions].

The experience of climatic health resorts at the Crimea sea shore has been summarized. The potential of climatic treatment can be fully realized only with the participation of therapeutic, rehabilitation and recreation institutions.

[Changes in the immunity indices of children suffering from inflammatory lung diseases undergoing cold exposures].

Cooling and heating the feet in children with pulmonary inflammatory diseases in remission improved immunity. Air baths in addition to stimulating immunity enhanced bactericidal potential of blood neutrophils. Cool and warm showers decreased this potential.

[The effect of cold exposure on the respiratory function in children suffering from inflammatory lung diseases].

Single total (air bath, douche) and local (cooling of the feet with water) exposure to mild cold do not arise noticeable disorders of the respiration function in children suffering from recurrent and asthmatic bronchitis in remission. Local cold procedures improve bronchial patency while the exposure to heat results in its worsening.

[The role of hypoxic-hypercapnic training in reducing local protective factors of the lung in a chronic inflammatory process in bronchopulmonary tissue under experimental conditions].

The effect of hypoxic-hypercapnic trainings (HHT), conducted before the induction of inflammation or started at next day after foreign body bringing, was shown in experiment on rats with chronic inflammation process in bronchopulmonary tissue. HHT conducted before the induction of inflammation in general had an favourable effect. HHT conducted later had a positive effect together with expressed negative changes, in particular worsening of inflammation process in lung.

[Changes in the clinical and immunological indices of patients with disseminated sclerosis being treated with prednisolone].

The authors examined clinically and immunologically 60 patients with multiple sclerosis. One group (30 patients) received prednisolone in the complex treatment while the second group (30 patients) received only complex treatment. A control group of 20 healthy persons was examined.

[Disruption of energy metabolism in the liver during lung inflammation in rats].

Low energetic shift was found in liver mitochondria of rats with pneumonia, which was expressed as a decrease in rates of phosphorylating respiration and ADP phosphorylation independently on substrate of oxidation. Training of rats under hypoxic conditions led to a decrease in severity and distribution of inflammation in the animals with induced pneumonia. Patterns of mitochondrial oxidation, if Krebs cycle substrates were used, were normalized in most of the animals, while rates of ADP-stimulated respiration, phosphorylation as well as energy regulation of respiration and efficiency of phosphorylating respiration were distinctly increased.

[The effect of hypoxia on oxidative phosphorylation and lipid peroxidation in rat liver mitochondria upon lung inflammation].

Hypoxic trainings of rats (maintenance in the test chamber at the "altitude" 4 km above the sea level for 7 hours a day for two weeks) prevent pneumonia-induced activation of peroxidation for lipids of the liver mitochondria. This increases the phosphorylating respiration rate when lipemic serum is used as an oxidation substrate (not succinate). In these experiments the efficiency of oxidative phosphorylation (delta ADP/delta O) when either succinate and glutamate or glutamate and malate have been oxidated corresponded to the values typical of intact animals and was higher when lipemic serum was used.

[The permeability of the hemato-encephalic barrier for metals in patients with bacterial meningoencephalitis and brain edema].

The authors investigated the quantitative content of a group of metals in the cerebrospinal fluid in 30 patients with bacterial meningoencephalitis with brain edema. It was established that selective permeability of the blood brain barrier has a compensatory character directed to maintenance of the mineral homeostasis of the central nervous system.

[Use of the methods of mathematical modeling for evaluation of the data of cerebrospinal fluid examination in patients with bacterial meningoencephalitis].

As many as 300 patients of different age groups underwent a probability statistical analysis of cytosis and CSF protein depending on the outcome of bacterial meningoencephalitis. The clinical and CSF interrelations discovered reflect the function of the blood-brain barrier and can be used as an additional test for predicting the disease outcome.

[The effect of prednisolone on the immunoglobulin content of the blood in patients with disseminated sclerosis].

The authors examined the dynamics of IgA, IgM, IgG in the blood of 100 patients with multiple sclerosis. Of them 50 patients received complex treatment in combination with prednisolone. Comparison of indices of concentration of serum immunoglobulins revealed a distinct reduction of their level of all classes in patients with multiple sclerosis, especially in those receiving prednisolone.

[Disordered lipid peroxidation and ATPase activities in patients with disseminated sclerosis].

Changes of ATP-ase activity and accumulation of lipid peroxidation products in patients with multiple sclerosis depended on the clinical course of the disease. Exacerbation is accompanied by an increased activity of Mg2(+)-dependent ATP-ase and reduction of Ca2(+)-dependent ATP-ase in the leucocytes. These changes were not revealed during remission.

[Characteristics of the course of bronchitis in allergy].

The course of bronchitis with clinical manifestations of allergy is characterized by certain cytochemical features which (as compared with bronchitis without allergy) show an increase of deoxyribonucleic acid, increased permeability of the intracellular membranes of polynuclear leucocytes and lymphocytes, reduction of the content of cationic proteins and activity of acid phosphatase in the polynuclear leucocytes of the blood.