Evaluating efflux pump inhibition in Staphylococcus aureus 1199B strain using thiadiazine-derived compounds: In vitro and in silico approaches.

Thiadiazines are heterocyclic compounds known for some pharmacological activities. However, the ability of these compounds and their derivatives to act as antibacterial agents and inhibitors of the efflux system in resistant bacteria remains unknown. This study aims to evaluate the antibacterial and NorA efflux pump inhibitory activities of thiadiazine-derived compounds (IJ14, IJ15, IJ16, IJ17, IJ18, IJ19, and IJ20) against the Staphylococcus aureus 1199B strain.

Assessment In vitro and In silico of the Activity of Thiadiazines as NorA Efflux Pump Inhibitors.

Antimicrobials fight microorganisms, preventing and treating infectious diseases. However, antimicrobial resistance (AMR) is a growing concern due to the inappropriate and excessive use of these drugs. Several mechanisms can lead to resistance, including efflux pumps such as the NorA pump in Staphylococcus aureus, which reduces the effectiveness of fluoroquinolones.

Potentiating-antibiotic activity and absorption, distribution, metabolism, excretion and toxicity properties (ADMET) analysis of synthetic thiadiazines against multi-drug resistant (MDR) strains.

Background: Thiadiazines are heterocyclic compounds that contain two nitrogen atoms and one sulfur atom in their structure. These synthetic molecules have several relevant pharmacological activities, such as antifungal, antibacterial, and antiparasitic.

Objectives: The present study aimed to evaluate the possible in vitro and in silico interactions of compounds derived from thiadiazines.

AI-Driven Discovery of SARS-CoV-2 Main Protease Fragment-like Inhibitors with Antiviral Activity .

SARS-CoV-2 is the causative agent of COVID-19 and is responsible for the current global pandemic. The viral genome contains 5 major open reading frames of which the largest ORF1ab codes for two polyproteins, pp1ab and pp1a, which are subsequently cleaved into 16 nonstructural proteins (nsp) by two viral cysteine proteases encoded within the polyproteins. The main protease (Mpro, nsp5) cleaves the majority of the nsp's, making it essential for viral replication and has been successfully targeted for the development of antivirals.

Development and validation of a video-assisted liver biopsy technique using a minimally-invasive device.

Background: Percutaneous liver biopsy is the gold standard technique for establishing the cause of cirrhosis and liver disease activity assessment. However, some cases of steatohepatitis or other chronic liver diseases show a high number of false negative results in samples obtained via the percutaneous route. This fact justifies performing a liver biopsy via the laparoscopic route.

Leukocyte metabolism in obese type 2 diabetic individuals associated with COVID-19 severity.

Recent studies show that the metabolic characteristics of different leukocytes, such as, lymphocytes, neutrophils, and macrophages, undergo changes both in the face of infection with SARS-CoV-2 and in obesity and type 2 diabetes mellitus (DM2) condition. Thus, the objective of this review is to establish a correlation between the metabolic changes caused in leukocytes in DM2 and obesity that may favor a worse prognosis during SARS-Cov-2 infection. Chronic inflammation and hyperglycemia, specific and usual characteristics of obesity and DM2, contributes for the SARS-CoV-2 replication and metabolic disturbances in different leukocytes, favoring the proinflammatory response of these cells.

The Body Adiposity Index is not applicable to the Brazilian adult population.

Background: Obesity is a serious disease that burdens public health systems around the world. It is a risk factor for the development of several non-communicable chronic diseases that are related to the amount and distribution of body fat. Body composition assessment using simple and low-cost techniques can help in the early detection of excess fat, allowing for the prevention and treatment of both obesity and associated diseases.

Development and validation of an instrument (CSII - Brazil) to assess users' conceptual and procedural knowledge of continuous subcutaneous infusion systems.

Objective: To develop, adapt and validate an instrument named "CSII - Brazil" to assess users' conceptual and procedural knowledge of continuous subcutaneous insulin infusion systems.

Methods: Methodological and exploratory study developed in three stages: a) instrument development; b) content validation and cultural adaptation (evaluation by a committee of experts and pre-test with CSII users); c) psychometric validation through instrument application in a sample of 60 patients by means of the web tool . Internal consistency and reproducibility analyses were performed within IBM SPSS Statistics 20 programming environment.

Colonic pseudotumor caused by abdominal actinomycosis: A case report.

We report a case of abdominal actinomycosis, a chronic suppurative infection caused by bacteria of the genus Actinomyces, simulating colon cancer, and presenting with abdominal pain and leukocytosis. Computed tomography revealed a mass lesion with irregular contours, infiltrative aspect, with extension to omental fat and abdominal wall, in the transverse colon. A surgical intervention was performed due to the suspicious of a colonic tumor.

Metabolic Cost of Paddling on Different Commercially Available Kayak Ergometers.

Purpose: To compare the metabolic cost of paddling on different commercially available kayak ergometers using a standardized kayak incremental exercise protocol.

Methods: Six male sprint kayak athletes undertook an incremental exercise protocol on 3 different kayak ergometers utilizing a randomized counterbalanced pair-matched design.

Results: Mean maximal aerobic power on the WEBA ergometer (265 [14] W) was significantly higher than on the Dansprint (238 [9] W) and KayakPro® (247 [21] W, P < .

The impact of vitamin D supplementation on VDR gene expression and body composition in monozygotic twins: randomized controlled trial.

Vitamin D supplementation is widely used. However, there is no consensus on the use and dosage of this supplement and the existing recommendations arise from studies based on the benefits that this nutrient can facilitate in bones. In addition, individual genetics can influence the response to supplementation, therefore, research involving monozygotic twins aims to reduce these differences in phenotypic responses.

The recurrence of the melanotic neuroectodermal tumour of infancy: an unusual presentation of a rare tumour.

The melanotic neuroectodermal tumour of infancy (MNTI), also known as melanotic progonoma, is a rare neoplasm derived from neural crest cells. Although it is fundamentally benign, the tumour may present a locally aggressive behaviour, characterised by a rapid progression and a destructive invasion of adjacent structures, hence causing deformities. Unfortunately, perhaps due to the low incidence of this type of tumour, the published cases in the literature do not characterise the factors that imply the malignant or recurrent behaviour of the disease, nor the therapy to conduct these cases.

Structures related to pheromone storage in alar androconia and the female abdominal scent gland of Heliconius erato phyllis, Heliconius ethilla narcaea, and Heliconius besckei (Lepidoptera: Nymphalidae: Heliconiinae).

We describe the morphology of alar androconia and the female abdominal scent gland of Heliconius erato phyllis, Heliconius ethilla narcaea, and Heliconius besckei. Androconial scales of Heliconius, which are arranged in overlapping wing bands, release pheromones during courtship, probably through vibratory movements of male wings over the female to induce her to mate. An antiaphrodisiac is produced by glands located in the valves of the male and is transferred during copulation to the yellow dorsal abdominal sac present in the virgin female, causing this sac to emit a scent that reduces the attractiveness of the female for courtship with other males.

Mice on menu: opportunistic feeding behaviour of the Amazonian silver arowana Osteoglossum bicirrhosum.

Two silver arowana, Osteoglossum bicirrhosum, one male (49.5 cm standard length, L ) and one female (52.5 cm L ) that had fed on small-eared colilargo or small-eared pygmy rice rat Oligoryzomys microtis, were sampled during the rising water period in the Brazilian Amazon.

Lipid overload during gestation and lactation can independently alter lipid homeostasis in offspring and promote metabolic impairment after new challenge to high-fat diet.

Background: Nutritional status in early life is critically involved in the metabolic phenotype of offspring. However the changes triggered by maternal consumption of high-fat diet (HFD) in pre- or postnatal period should be better understood. Here we evaluated whether maternal HFD consumption during gestation and lactation could differently affect liver miR-122 and miR-370 expression leading to metabolic damages observed in offspring.

TRPA1, substance P, histamine and 5-hydroxytryptamine interact in an interdependent way to induce nociception.

Background: Although TRPA1, SP, histamine and 5-hydroxytryptamine (5-HT) have recognized contribution to nociceptive mechanisms, little is known about how they interact with each other to mediate inflammatory pain in vivo. In this study we evaluated whether TRPA1, SP, histamine and 5-HT interact, in an interdependent way, to induce nociception in vivo.

Methods And Results: The subcutaneous injection of the TRPA1 agonist allyl isothiocyanate (AITC) into the rat's hind paw induced a dose-dependent and short lasting behavioral nociceptive response that was blocked by the co-administration of the TRPA1 antagonist, HC030031, or by the pretreatment with antisense ODN against TRPA1.

Direct Method for Determination of Al, Cd, Cu, and Pb in Beers In Situ Digested by GF AAS Using Permanent Modifiers.

The object of the present study was to development of safe, reliable fast, and efficient methodologies for the direct determination of Al, Pb, Cu, and Cd in non-digested beer samples of widely consumed brand name beers sold in Brazil, using graphite furnace absorption spectrometry (GF AAS). Pyrolysis and atomization temperature curves of selected chemical modifiers (iridium, rhodium, ruthenium, zirconium, and non-modifier use) were used to stabilize each metal and sensitivity in a beer sample was diluted to 1:1 with 0.2 % v/v nitric acid after degasification.

Is Weiner's attribution-help model stable across cultures? A test in Brazilian subcultures.

The effect of attribution of responsibility on the intention to help is a well-studied effect. The Weiner model of attribution-emotion-help is the main theoretical development to describe the structural relation between these variables. Some research results have suggested that culture influences the relation between model variables.

Association between anthropometry and upper-body strength qualities with sprint paddling performance in competitive wave surfers.

This study aimed to evaluate the potential association with anthropometry and upper-body pulling strength with sprint kinematics of competitive surfers. Ten competitive male surfers (23.9 ± 6.

Radiosurgery for brain metastases: who may not benefit?

Purpose: To select a group of patients with brain metastases for whom stereotactic radiosurgery (SRS) may not be beneficial.

Patients, Materials, And Methods: Actuarial survival of 87 patients with brain metastases treated with SRS between July 1993 and May 1999 was retrospectively analyzed under stratification by the Score Index for Stereotactic Radiosurgery for Brain Metastases (SIR). To identify the group of patients most likely to survive less than 6 months after SRS, Cox model survival curves were calculated for all SIR values, and Kaplan-Meier survival curves were calculated for two SIR subsets (0-5 and 6-10) and were compared by log-rank test.

Radiosurgery for brain metastases: a score index for predicting prognosis.

Purpose: To analyze a prognostic score index for patients with brain metastases submitted to stereotactic radiosurgery (the Score Index for Radiosurgery in Brain Metastases [SIR]).

Methods And Materials: Actuarial survival of 65 brain metastases patients treated with radiosurgery between July 1993 and December 1997 was retrospectively analyzed. Prognostic factors included age, Karnofsky performance status (KPS), extracranial disease status, number of brain lesions, largest brain lesion volume, lesions site, and receiving or not whole brain irradiation.

[Treatment of verruca with DNCB].

The authors analyse the use of topical DNCB in the treatment of warts evaluating the influence of lesions duration and its number on the results which were obtained. They came to the conclusion that in the 29 cases which were studied, the number of lesions had no influence at all. The shorter the duration of a lesion was, the better the results obtained were.