Extraction, Isolation, Identification, and Characterization of Anthocyanin from Banana Inflorescence by Liquid Chromatography-Mass Spectroscopy and Its pH Sensitivity.

Anthocyanin is an important flavonoid with antioxidant, anticancer, and anti-inflammatory properties. This research investigates the anthocyanin content of Cavendish banana inflorescence, a by-product often discarded as agricultural waste. The study employs two drying methods, namely oven-drying and freeze-drying, followed by accelerated solvent extraction using acidified water and methanol.

Pretreated sugarcane bagasse matches performance of synthetic media for lipid production with Yarrowia lipolytica.

Engineered strains of Yarrowia lipolytica with modified lipid profiles and other desirable properties for microbial oil production are widely reported but are almost exclusively characterized in synthetic laboratory-grade media. Ensuring translatable performance between synthetic media and industrially scalable lignocellulosic feedstocks is a critical challenge. Yarrowia lipolytica growth and lipid production were characterized in media derived from two-step acid-catalyzed glycerol pretreatment of sugarcane bagasse.

A review on modelling of thermochemical processing of biomass for biofuels and prospects of artificial intelligence-enhanced approaches.

Biofuels from lignocellulosic biomass converted via thermochemical technologies can be renewable and sustainable, which makes them promising as alternatives to conventional fossil fuels. Prior to building industrial-scale thermochemical conversion plants, computational models are used to simulate process flows and conditions, conduct feasibility studies, and analyse process and business risk. This paper aims to provide an overview of the current state of the art in modelling thermochemical conversion of lignocellulosic biomass.

Effects of lignocellulosic biomass type on the economics of hydrothermal treatment of digested sludge for solid fuel and soil amendment applications.

Digested sludge is a waste stream from anaerobic digestion (AD) in wastewater treatment plants. Hydrothermal treatment (HTT) of sludge mixed with lignocellulosic biomass is an attractive approach to improve sludge dewaterability and generate value-added products. However, process economics has not been well understood.

Triple action of FeCl-assisted hydrothermal treatment of digested sludge for deep dewatering.

In this study, a FeCl-assisted hydrothermal treatment (HTT) process under mild conditions (90 °C-130 °C) was developed for deep dewatering of anaerobically digested sludge. HTT of sludge at 90 °C-130 °C with 4%-6% Fe ions loading based on total sludge solids followed by mechanical dewatering reduced sludge water content from 82% to 38%-53% and sludge weight by 62%-72%. The treatment increased the flowability of sludge through reduction of apparent viscosity and disintegration of colloidal forces between sludge particles.

Effects of hydrochar derived from hydrothermal treatment of sludge and lignocellulose mixtures on soil properties, nitrogen transformation, and greenhouse gases emissions.

In this study, hydrochar samples derived from hydrothermal treatment (HTT) of sludge and sludge-biomass mixtures were applied to a sandy soil and their effects on soil properties, soil nutrients, greenhouse gas (GHG) emissions, and soluble heavy metals were investigated. The application of untreated sludge and hydrochar derived from HTT of sludge at 180 °C led to the highest soluble nitrate, CO and NO emissions, followed by the application of hydrochar samples derived from HTT of sludge-biomass mixtures at 180 °C. Although the application of hydrochar samples derived from HTT of sludge alone and sludge-biomass mixtures at 240 °C in sandy soil led to the lowest emissions of CO and NO, it resulted in lower levels of soil electrical conductivity (EC), cation exchange capacity (CEC) and soluble phosphorus.

Effects of lignocellulosic biomass type on nutrient recovery and heavy metal removal from digested sludge by hydrothermal treatment.

Sludge is a nutrient-rich organic waste generated from wastewater treatment plants. However, the application of sludge as a nutrient source is limited by its high contents of water and pollutants. In this study, the effects of biomass type on nutrient recovery and heavy metal removal from digested sludge by hydrothermal treatment (HTT) were investigated.

Filamentous fungi for future functional food and feed.

In this review, we offer our opinion of current and expected trends regarding the use of mushrooms and mycelia in food and feed. Mushrooms have provided food for millennia and production methods and species diversity have recently expanded. Beyond mushrooms, cultured fungal mycelia are now harvested as a primary product for food.

Effects of pretreatment methods on biomethane production kinetics and microbial community by solid state anaerobic digestion of sugarcane trash.

Solid state anaerobic digestion (SS-AD) of lignocellulose is effective in improving biomethane productivity but is limited by low biomass digestibility and lack of substrate-specific working microorganisms. In this study, the effects of different pretreatment methods on biomethane production by SS-AD of sugarcane trash were studied. The biomethane production, fitted to a modified Gompertz's model, predicted a maximum methane yield of 214.

Effect of hydrothermal treatment on deep dewatering of digested sludge: Further understanding the role of lignocellulosic biomass.

In this study, lignocellulose-assisted hydrothermal treatment (HTT) of digestated sludge was studied to further understand the role of biomass in HTT and its effect on subsequent sludge dewatering. HTT of sludge-biomass mixtures at 180 °C for 60 min at a sludge/biomass total solids (TS) ratio of 1:1 led to solid residue moistures of 36%-40% after dewatering using a hydraulic press at 24 MPa, compared to 69.5% without biomass.

Transformation of sugarcane molasses into fructooligosaccharides with enhanced prebiotic activity using whole-cell biocatalysts from Aureobasidium pullulans FRR 5284 and an invertase-deficient Saccharomyces cerevisiae 1403-7A.

Fructooligosaccharides (FOS) can be used as feed prebiotics, but are limited by high production costs. In this study, low-cost sugarcane molasses was used to produce whole-cell biocatalysts containing transfructosylating enzymes by Aureobasidium pullulans FRR 5284, followed by FOS production from molasses using the whole-cells of A. pullulans.

Effect of ferrous iron loading on dewaterability, heavy metal removal and bacterial community of digested sludge by Acidithiobacillus ferrooxidans.

Acidithiobacillus ferrooxidans ILS-2 was adapted in digested sludge and used to treat sludge for dewaterability improvement. Results showed that increasing ferrous iron loading increased sludge dewaterability, but the inoculation of the bioleaching strain had little effect on sludge dewaterability compared to controls without the strain. The total extracellular polymeric substances (EPS) contents of sludges with and without bioleaching treatment were similar except for bioleaching treatment at 10% ferrous iron loading (on sludge total solids) where total EPS was higher with bioleaching treatment.

Highly efficient production of transfructosylating enzymes using low-cost sugarcane molasses by A. pullulans FRR 5284.

Fructooligosaccharides (FOS) are a type of important prebiotics and produced by transfructosylating enzymes. In this study, sugarcane molasses was used as the substrate for production of transfructosylating enzymes by Aureobasidium pullulans FRR 5284. NaNO was a superior nitrogen source to yeast extract for production of transfructosylating enzymes by A.

A systematic evaluation of biomethane production from sugarcane trash pretreated by different methods.

Biomethane production was systematically evaluated with sugarcane trash pretreated by liquid hot water (LHW), dilute acid (DA) and KOH solutions. Multiple linear regression analysis identified glucan in pretreated solid residue as well as C5 sugars and acetic acid in pretreatment hydrolysate as the key parameters affecting biomethane potentials. Moreover, biomethane production was best simulated using Chen & Hashimoto model with a predicted highest methane yield of 187 mL/g initial total solids (TS) based on LHW (130 °C for 15 min) and KOH (10% on trash, 150 °C for 60 min) pretreatments.

Mild fractionation of sugarcane bagasse into fermentable sugars and β-O-4 linkage-rich lignin based on acid-catalysed crude glycerol pretreatment.

Acid-catalysed crude glycerol (ACG) pretreatment was carried out at 110 °C and 130 °C for mild fractionation of sugarcane bagasse into fermentable sugars and high-quality lignin. ACG pretreatment at 110 °C led to sugar yields of 71%-74%, comparable to those with acid-catalysed reagent-grade glycerol (AG). ACG pretreatment removed more lignin (53%-75%) than AG pretreatment (38%-49%), likely due to the presence of organic impurities in ACG.

Scale-up of two-step acid-catalysed glycerol pretreatment for production of oleaginous yeast biomass from sugarcane bagasse by Rhodosporidium toruloides.

Two-step dilute acid and acid-catalysed glycerol pretreatment was developed to maximise sugar yield from sugarcane bagasse. At the laboratory scale, dilute acid pretreatment at 130 °C followed by acid-catalysed glycerol pretreatment at 170 °C led to a total sugar (C5 + C6) yield of 82%, 31% higher than that from one-step acid-catalysed glycerol pretreatment. At the pilot scale, the two-step dilute acid and acid-catalysed glycerol pretreatment led to a maximum sugar yield of 74%, 13% higher than that from one-step pretreatment with 52% reduction in glycerol usage.

A snapshot of microbial diversity and function in an undisturbed sugarcane bagasse pile.

Background: Sugarcane bagasse is a major source of lignocellulosic biomass, yet its economic potential is not fully realised. To add value to bagasse, processing is needed to gain access to the embodied recalcitrant biomaterials. When bagasse is stored in piles in the open for long periods it is colonised by microbes originating from the sugarcane, the soil nearby or spores in the environment.

Co-utilization of acidified glycerol pretreated-sugarcane bagasse for microbial oil production by a novel strain.

Acidified glycerol pretreatment is very effective to deconstruct lignocellulosics for producing glucose. Co-utilization of pretreated biomass and residual glycerol to bioproducts could reduce the costs associated with biomass wash and solvent recovery. In this study, a novel strain RP 15, isolated from sugarcane bagasse, was selected and tested for coconversion of pretreated biomass and residual glycerol to microbial oils.

Microbial oil production from acidified glycerol pretreated sugarcane bagasse by .

An integrated microbial oil production process consisting of acidified glycerol pretreatment of sugarcane bagasse, enzymatic hydrolysis, microbial oil production by NRRL 1757 and oil recovery by hydrothermal liquefaction (HTL) of fungal biomass in fermentation broth was assessed in this study. Following pretreatment, the effect of residual pretreatment hydrolysate (containing glycerol) on enzymatic hydrolysis was firstly studied. The residual pretreatment hydrolysate (corresponding to 2.

Biofuels from food processing wastes.

Food processing industry generates substantial high organic wastes along with high energy uses. The recovery of food processing wastes as renewable energy sources represents a sustainable option for the substitution of fossil energy, contributing to the transition of food sector towards a low-carbon economy. This article reviews the latest research progress on biofuel production using food processing wastes.

Effects of glycerol on enzymatic hydrolysis and ethanol production using sugarcane bagasse pretreated by acidified glycerol solution.

In this study, for the first time the effects of glycerol on enzymatic hydrolysis and ethanol fermentation were investigated. Enzymatic hydrolysis was inhibited slightly with 2.0 wt% glycerol, leading to reduction in glucan digestibility from 84.

A novel population balance model for the dilute acid hydrolysis of hemicellulose.

Background: Acid hydrolysis is a popular pretreatment for removing hemicellulose from lignocelluloses in order to produce a digestible substrate for enzymatic saccharification. In this work, a novel model for the dilute acid hydrolysis of hemicellulose within sugarcane bagasse is presented and calibrated against experimental oligomer profiles. The efficacy of mathematical models as hydrolysis yield predictors and as vehicles for investigating the mechanisms of acid hydrolysis is also examined.

A multi-criteria analysis approach for ranking and selection of microorganisms for the production of oils for biodiesel production.

Oleaginous microorganisms have potential to be used to produce oils as alternative feedstock for biodiesel production. Microalgae (Chlorella protothecoides and Chlorella zofingiensis), yeasts (Cryptococcus albidus and Rhodotorula mucilaginosa), and fungi (Aspergillus oryzae and Mucor plumbeus) were investigated for their ability to produce oil from glucose, xylose and glycerol. Multi-criteria analysis (MCA) using analytic hierarchy process (AHP) and preference ranking organization method for the enrichment of evaluations (PROMETHEE) with graphical analysis for interactive aid (GAIA), was used to rank and select the preferred microorganisms for oil production for biodiesel application.

The combination of plant-expressed cellobiohydrolase and low dosages of cellulases for the hydrolysis of sugar cane bagasse.

Background: The expression of biomass-degrading enzymes (such as cellobiohydrolases) in transgenic plants has the potential to reduce the costs of biomass saccharification by providing a source of enzymes to supplement commercial cellulase mixtures. Cellobiohydrolases are the main enzymes in commercial cellulase mixtures. In the present study, a cellobiohydrolase was expressed in transgenic corn stover leaf and assessed as an additive for two commercial cellulase mixtures for the saccharification of pretreated sugar cane bagasse obtained by different processes.

Stability of endoglucanases from mesophilic fungus and thermophilic bacterium in acidified polyols.

Recent developments in chemical pretreatments of lignocellulosic biomass using polyols as co-solvents (e.g., glycerol and ethylene glycol) at temperatures less than 100°C may allow the effective use of thermostable and non-thermostable cellulases in situ during the saccharification process.