Ionic Strength, Surface Charge, and Packing Density Effects on the Properties of Peptide Self-Assembled Monolayers.

The various environmental parameters of packing density, ionic strength, and solution charge were examined for their effects on the properties of the immobilized peptide mimotope CH19 (CGSGSGSQLGPYELWELSH) that binds with the therapeutic antibody Trastuzumab (Herceptin) on a gold substrate. The immobilization of CH19 onto gold was examined with a quartz crystal microbalance (QCM). The QCM data showed the presence of intermolecular interactions resulting in the increase of viscoelastic properties of the peptide self-assembled monolayer (SAM).

An ESR and DFT study of hydration of the 2'-deoxyuridine-5-yl radical: a possible hydroxyl radical intermediate.

The mechanism of radiation-induced frank strand break formation in irradiated 5-bromo-2'-deoxyuridine (BrdU)-labelled DNA is still unclear despite the proven radiosensitizing properties of BrdU. Combination of ESR spectroscopy and quantum chemical modelling points to a simple reaction between the uridine-5-yl radical and water molecules that produces the genotoxic hydroxyl radical.