[Development of technology for isolation and purification of gentamicin using sorption without filtration].

A technology for isolation and purification of gentamicin from nonfiltered fermentation broth with the use of sorption-pulsory columns was developed as a result of the research work. The practical application of the technology will increase the yield from 30 to 57 per cent and reduce the content of the minor admixtures in the final product up to 5 per cent in comparison to the currently used process.

[Sorption of aminoglycoside antibiotics from unfiltered culture fluid].

Sorption of aminoglycosides such as sisomicin, gentamicin, kanamycin and streptomycin from the non-filtrated fermentation broth by carboxylic cation exchange resins under the static conditions was studied. The optimal conditions for the sorption of the aminoglycosides and their subsequent desorption were determined (the ratio of the volumes of the sorbent and fermentation broth and the time of the sorption and desorption).

[Microcalorimetric study of heat denaturation of DNA from calf thymus DNA in the absence of magnesium ions].

Thermal denaturation was studied for a wide range of magnesium ions concentrations and salt concentration 0.15 M NaCl. It was shown that thermal stability of DNA increases at low Mg/2P ratios and decreases at high concentrations of magnesium ions.

[Sorption of aminoglycoside antibiotics by weakly acidic cation exchangers].

Sorption of aminoglycoside antibiotics close by their chemical structures such as gentamicin, sisomicin and kanamycin by polyacrylic and polymetaacrylic cation exchange resins was studied. The generality of the sorption process (kinetics and statics) on the carboxylic cation exchange resins with participation of the ions of the aminoglycoside antibiotics was shown.