Radiation-induced cell cycle arrests in Ehrlich ascites carcinoma cells in vivo.

Radiation-induced progression delay in G(1)/S, S and G(2)/M phases of p53 wild-type Ehrlich ascites carcinoma (EAC) cells growing in vivo was investigated by DNA flow cytometry. Different behavior patterns of EAC cells at the time after irradiation with low (2, 4, 6, 8 Gy) and high (10, 15, 20 Gy) doses were evaluated. While EAC cells showed a small progression delay in S phase and a dose-dependent block in G(2)/M phase after the irradiation with low doses, a clear additional block in G(1)/S phase was observed after irradiation with high doses.

[The combained effects of the long-term gamma-irradiation and heavy metall ions on the haematopoietic system of rats].

The combiened effects of different dose rates (0.625 microGy/s - 1.1 mGy/s) of gamma-irradiation and of cuprum and of cadmium ions on the haematopoietic system of rats were studied.

[Cerebrospinal fluid sorption in patients with heroin addiction].

The sorption of cerebrospinal fluid (CSF) was attempted as a special detoxifying procedure in a group of sixty heroine addicts. CSF contents of cells, total protein, nucleic acids and interleukin-1 (IL-1), as well as acridine orange (AO) binding to CSF cells were determined before and after the procedure. The treatment provided immediate clinical improvement for 70% of the patients.

[The effect of soil extracts with different heavy metal levels on the viability of isolated blood system cells].

Short-term incubation of rat thymocytes, bone marrow cells, and macrophages with aqueous extracts of soil demonstrated positive correlations between damage to the cells and increased levels of copper, chromium, and manganese in the soil, while increased levels of zinc and lanthanum were associated with less pronounced changes in the cells.

[Effect of chronic irradiation on the cell cycle of rat myelokaryocytes].

Rats were exposed to gamma-radiation within dose range from 0.12 to 4.0 Gy at dose rates from 0.

[The possibility of using the population characteristics of myelokaryocytes for the early diagnosis of radiation lesions].

The method of flow cytofluorimetry was used to study some population characteristics of bone marrow cells of control and irradiated rats. The simultaneous recording of cellularity and distribution of myelokaryocytes among the cell cycle phases was shown to give reliable results for determining the extent to which the organism was exposed at early times following irradiation.

[The mechanism of the formation of a circadian rhythm of bone marrow proliferation in rats].

Flow cytofluorimetry and statmokinetic method were used to study the circadian rhythm of bone marrow proliferation in Pliss' lymphosarcoma-bearing and intact rats. These data were compared to those obtained in the study of the mitotic activity of the bone marrow in cancer patients. It was found that, already at early stage, tumor affected the circadian rhythm of bone marrow proliferation, reducing the amplitude of oscillations.

[Pulse flow cytofluorimeter for the UV region of the spectrum].

A high-speed flow system for the automatic analysis of cells has been described. This device can register the fluorescence staining and ultra-violet fluorescence intensity of cells. The rate of measurement is more than 1000 cells per second.

[Possibility of using cytofluorometers to determine the ratio between normal and neoplastic cells].

Principal possibility to use the pulsatile flowtype cytofluorometers for recording nontypical cells in biopsy material and the whole blood is considered.

[Flow-type pulsatile cytofluorometer with increased sensitivity].

A new cytofluorometer is described, with sensitivity twice as high as that of the existing units.

[Method of flow impulse cytofluorometry in the UV spectrum for the study of tumor cells in the whole blood].

Under consideration is the principal opportunity of using impulse cytofluorometers working in the UV spectrum for recording tumor cells in blood. The results of model experiments evidence the advantages of this method, however at present its sensitivity is not adequate enough.

[Study of mouse liver cell phosphorescence at deep cooling].

The spectrum and decay curves of phosphorescence of mouse liver cells at --180 degrees C was studied using the phosphorescence microscope. The phosphorescence studied was shown to involve two components with different life spans. Part of either component in the total spectrum is estimated.

[Apparatus for automatic recording of the intensity of ultraviolet fluorescence from individual cells].

The device enables to examine about 40 000--60 000 cells per min. The results are presented by the distribution curves of the ultraviolet fluorescence intensity.