[Possibilities of culturing Cryptosporidia in the cell-free medium].

An attempt was made to culture the Cryptosporidia, which had been isolated from the excreta of 24 HIV-infected patients and prepassaged on SCID mice, in an artificial host cell-free medium. The authors examined their impact of rabbit hepatic and renal tissue extracts, hemolyzed rabbit blood, bovine bile, ATP, calves' embryonic serum, bovine serum albumin, glucose, phytohemagglutinine, and concanavalin A, cultivation temperature, and medium pH on growth culture. A total of 72 cultures were tested.