[Basic characteristics of a live influenza vaccine for children from type A influenza (H1N1 and H3N2) used separately and in combination].

The study of safety and immunizing properties of a live influenza vaccine from influenza viruses type A (H1N1) and A (H3N2) used separately and in combination was carried out in 2461 children ranging in age from 3 to 15 years. The possibility of combining two influenza type A viruses with similar biological activity was demonstrated. The vaccine exerted no harmful effect in children and actively stimulated the production of antihemagglutinating, antineuraminidase, and secretory antibodies and enhanced the formation of cell-mediated immunity.

[Immunization with a complex preparation: a new method of influenza prevention. The basis for using a complex preparation for preventing influenza].

A combined preparation for influenza prevention (CPIP) consisting of an interferon inducer stimulating immunogenesis and killed influenza vaccine is proposed. Twenty five inducers-stimulators have been tested: polynucleotides, polysaccharide and lipopolysaccharide extracted from Salmonella typhosa. Intranasal administration of CPIP to laboratory animals markedly stimulates interferon, secretory, and circulating antibody synthesis.

[Influenza virus variability and human sensitivity].

The data from the literature concerning the limited number of influenza A virus variants pathogenic for man are analysed. The possibilities of employing these theoretical concepts for improvement of the methods for virus investigations and epidemiological prognosing of epidemics, as well as the possibilities of creating a species-specific preparation for influenza A prevention are discussed.

[Anti-infective activity of anti-influenza antibodies from human colostrum].

Antibodies against influenza virus of A, B serological types and PC-virus were detected in the colostrum collected after an epidemic. These antibodies belonged to the secretory IgA form. The secretory antibodies preparation made of colostrum, and its IgA fraction instilled intransally to mice and rats prevented the development of infection caused by 100--10 ID50/0.

[Participation of secretory antibodies in the protection from influenza].

Two collective bodies (319 persons in all) were under observation from October, 1974 to March, 1975 for the purpose of studying the problem on the participation of antibodies of the respiratory tract secretion in the protection from influenza virus, type A, infection. During the influenza epidemic outbreak there was revealed a reverse relationship between the antibody concentration in the nasal cavity secretion and the incidence of the disease, and also an interaction between the antibodies of the secretions and the serum in the influenza protection.

[Use of the indirect hemagglutination reaction for the express diagnosis of influenza outbreaks].

A new method for express diagnosis of influenza is described. The method is based on agglutination of antibody-sensitized erythrocytes in the presence of virus contained in nasal washings. Fourteen outbreaks in institutions and 113 patients in appartment foci were examined.

[Action of different cell types in influenza antibody production. 5. Keeping the immunologic memory to influenza virus A serotype by B- and T-lymphocytes].

The authors present data on the cooperation of the B- and T-lymphocytes in secondary immunological response to the antigens of influenza virus of serological type A. Cells of the spleen, bone marrow and the thymus in various combinations from the intact and immunized animals were transplanted to sublethally irradiated recipients; influenza virus was administered at the same time. Immunological memory to the virus antigens of serological type A proved to be preserved both by the B- and T-lymphocytes.

[Interaction of different types of cells in production of anti-influenzal antibodies. IV. Stimulation of B-lymphocytes by several biologically active substances].

Various biologicall-active preparations such as extracts of syngenous and allogenous thymus and LP S of Gram negative bacteria were administered to sublethally irradiated mice ascertain a possibility of replacement of T-lymphocytes in the production of antibodies to influenza virus. It appeared that the function of T-lymphocytes participating in the production of antibodies to influenza virus in mice could be replaced by the administration to these animals of extracts of the syngenous and allogenous thymus and LP S of Gram negative bacteria.

[Formation of secretory antibodies in experimental influenza infection].

The principal peculiarities attending formation of specific secretory immunity in experimental influenza infection were marked individual variability and a relative autonomicity of accumulation of the secretory antibodies. Functional condition of the secretory immunity before the infection influenced the formation of antibodies in the secretions and the blood sera: when the concentration of the secretory antibodies before the infection constituted 1 : 8--1 : 16, general and secretory antibodies accumulated less intensively. There was a progressive fall of the concentration of the secretory immunoglobulin A in the majority of the volunteers the first 3 weeks after the infection.

[Interaction of various types of cells in the production of anti-influenzal antibodies. III. Cooperation of B- and T-lymphocytes in the production of antibodies to the influenza virus].

Bone marrow and thymus cells of syngenous donors were transplanted to sublethally irradiated mice to study the interaction between the B- and T-lymphocytes in the production of antibodies to the influenza virus. Influenza virus proved to be referred to the thymus-dependent antigens; both types of the lymphocytes participated in the antibody production. The T-lymphocyte function could not be replaced by an increase in the dose of the antigen and of the amount of B-lymphocytes.

[Interrelationships between serum and secretory antibodies in immunization with live influenza vaccine].

A study was made of the accumulation of antibodies in the blood serum and the secretions of the respiratory tracts of persons immunized with the living influenza vaccine. The duration of inductive phase and the dynamics of the antibody accumulation in the secretions occurred irrespective of their initial level in the blood sera, this pointing to the autonomic character of the local immunity system. On the other hand the functional condition of the system of local immunity influenced the intensity of the antibody formation in the system of the general immunity.

[Interaction of cells of the lymphoid organs in the production of antibodies against the influenza virus. Report 1. Localization of the virus in the cells of the spleen].

A study was made of localization of crude and inactivated influenza virus injected intravenously and intraperitoneally. Macrophages were found to engulf the virus penetrating into the spleen; this was shown by the quantitative and qualitative immunofluorescent method, elcetron microscopy and inoculation to syngenous recipients of homogenates of macrophagal and lymphocytic fractions of splenic cells. No virus was revealed in the lymphocytes.