[Structure of peptide fragments of the complex (Lys(Abz)26) neurotoxin II from Naja naja oxiana cross-linked with the nicotinic acetylcholine receptor from Torpedo californica].

After irradiating the acetylcholine receptor complex with the title neurotoxin derivative, the labeled delta-subunit was separated by preparative SDS-PAGE, reduced-carboxymethylated and cleaved with LysC endoproteinase. One of the radioactive peptides isolated by HPLC was further purified by electrophoresis in a tricin gel. Edman degradation of the radioactive fractions yielded the sequence of the delta-subunit fragment starting from Phe148.